犬六联苗过敏的救治

笔者用犬六联苗对1037头宠物犬进行预防注射,免疫后发生过敏反应的有8例,约占免疫注射率的0.65%:其中发生严重过敏反应的1例,占免疫注射率的0.08%.     

杜仲人工林中的邻体干扰

本文研究了四川地区杜仲（Eucommia ulmoides）人工林，杜仲个体生长速度与邻体效应之间的相互关系。以杜仲个体为基株，3m为半径圆内的个体均被定久为邻体，采用张大勇改进的干扰指九模型和杜种的生长速度进行回归分析，发现杜仲生长速度与邻体干扰指数之间有显著的线性关系，研究结果表明，杜仲林内，杜仲个体之间表现为强烈的种内竞争，个体生长的动力来自杜仲各个体对资源竞争所引起的自疏作用，邻体干扰效应的存在是确定杜仲人工林合理经营密度的重要理论依据。     

用电子显微镜研究干法无胶硬质纤维板的结构

用扫描电子显微镜对未浸水和浸水后的干法无胶硬质纤维板进行观察，以获得有关无胶纤维板内在结构的变化，这可以更好地了解无胶纤维板的强度和耐水性能，在制造纤维板过程中，通过热磨使木材纤维在胞间层分离，并在热压时受压力作用再次结合，纤维重新结合的状况决定了纤维板的物理力学性能，经电镜观察的无胶纤维板，纤维间相互交织且结合紧密，即使是浸水后，纤维间的结合仍未出现松散现象，这表明不施加胶粘剂制造的干法硬质纤维板可以具有优越的强度和耐水性能。     

丽江市油橄榄产业发展现状、问题及对策分析

指出了丽江市油橄榄产业发展在气候条件、市场等方面有很大优势.但还存在着科研配备不完善、高层次研究人才缺乏、龙头企业带动效应未能体现等制约因素.针对丽江市油橄榄产业的发展现状 ,提出了建议和对策 ,为丽江市油橄榄产业的健康发展提供参考.     

基于BSA-Seq技术的甜瓜抗霜霉病InDel标记开发

目的 获得与甜瓜抗霜霉病基因连锁的分子标记或功能标记,用于甜瓜分子标记辅助选择育种。为进一步克隆甜瓜抗霜霉病主效基因奠定基础。方法 以野生高抗霜霉病资源DM-4和感病自交系DM-2为亲本,构建F₂代分离群体,利用BSA-seq对甜瓜抗霜霉基因进行QTL定位,开发InDel分子标记,并在双亲及其F₂群体单株进行筛选验证。结果 甜瓜抗霜霉病主效QTL位于第9号染色体。在候选区间开发了37个InDel分子标记,有9个PCR产物大小在抗、感双亲表现出差异,用F₂单株验证,结合田间表型鉴定结果,引物InDel 15和InDel 20准确率分别为95 %和98 %,引物InDel 15和InDel 20在抗病亲本中扩增产物大小分别为251和349 bp,在感病亲本中分别为231和324 bp。结论 引物InDel 15和InDel 20可以作为分子标记进行抗霜霉病辅助选育,加快了甜瓜抗霜霉病育种速度。     

不同品种与播期对麦棉套作棉花生长、产量及棉铃空间分布的影响

【目的】在麦棉两熟种植模式下,研究不同品种与播期对棉花生长、产量以及棉铃空间分布的影响,为棉花播期及品种选育提供依据。【方法】在大田试验中,设置裂区试验,以2个早熟棉品种锦科707和鲁棉2387为主区,4个播期5月10日(SD₁),5月20日(SD₂),5月30日(SD₃),6月10日(SD₄)为副区,分析不同品种和播期对棉花生育期进程、叶面积指数、干物质积累、棉株内部铃重及空间分布的影响。【结果】(1)随着播期的推迟,2个品种均表现为苗期不变,蕾期缩短,开花期和吐絮期先缩短后延长的趋势,锦科707在SD₁处理,鲁棉2387在SD₂处理下有利于营养器官向生殖器官转移;(2)品种和播期对棉花产量存在显著的互作效应,锦科707在SD₁处理下皮棉产量最高,为1 873 kg/hm²,鲁棉2387在SD₂处理下皮棉产量最高,为1 767 kg /hm²;(3)锦科707在SD₁处理中,上部和中部铃重最大,而下部铃重SD₃最大,鲁棉2387在SD₂处理中,下部、中部、上部铃重均高于其他处理。同一品种不同播期间,下部和中部成铃分布比例随播期的推迟而增加,而上部则成铃分布比例随播期的推迟而降低。【结论】在黄河流域麦棉套作种植模式下,过晚播种会导致产量严重降低,锦科707在5月10日,鲁棉2387在5月20日左右播种较为适宜。     

以油菜秸秆为主要原料的有机肥发酵工艺研究

采用堆肥工艺将农作物秸秆转化为有机肥还田是秸秆资源化利用的有效手段。本文以油菜秸秆为主要原料,通过添加腐熟菌剂及调节发酵原料组分,研究油菜秸秆腐熟发酵最佳工艺。结果表明:腐熟菌剂、碳氮比及氮源对油菜秸秆腐熟发酵影响显著,其中油菜秸秆添加腐熟菌剂和15%菜籽枯,发酵物料碳氮比为25∶1,堆肥效果较好,发芽率指数为73.5%。     

The effects of different types of management,functions, and characteristics of stands in Polish forests on the amount of coarse woody debris

In the modern forestry paradigm, many factors influence the amount of coarse woody debris (CWD). The present paper analyzes the effects of both local (national) programs (special functions of forests) and European programs (Natura 2000 sites), as well as the individual characteristics of forest stands. The study was conducted on 2,752 sampling plots distributed over an area of about 17,500 ha and located in lowland stands having a species composition typical of large areas in central Europe. Natura 2000 areas contained significantly more CWD (8.4 m³/ha) than areas not covered by the program (4.8 m³/ha). However, this is due to the fact that Natura 2000 sites involve well-preserved forest areas, such as nature reserves (26.6 m³/ha). In the managed forests that have been covered by the Natura 2000 program over the past several years, the volume of CWD has not increased. Forests with ecological and social functions differed slightly in the amount of CWD. More CWD occurred in protected animal areas (8.7 m³/ha) than in stands damaged by industry (3.9 m³/ha). Intermediate CWD levels were found in water-protection forests and in forests located around cities and military facilities. In managed forests, the lowest CWD volume was observed in middle-aged stands. The species composition of the stand had little effect on the volume of CWD. Only stands with a predominance of ash and alder had higher CWD levels (13.5 m³/ha). More CWD was found in stands whose species composition did not represent the potential site quality (6.4 m³/ha) than in habitats with the optimum species composition (3.8 m³/ha). CWD volume should be systematically increased taking into consideration local natural conditions. Such efforts should be focused on particularly valuable regions, and especially on Natura 2000 sites, where the threshold values reported from other European forests should be reached. Leaving some trees to die naturally and retaining reasonable amounts of such trees ought to be incorporated into CWD management practice in Poland.     

Protection of Procambarus clarkii against white spot syndrome virus using recombinant subunit injection vaccine expressed in Pichia pastoris

ABSTRACT:   The potentiality of injection vaccine against white spot syndrome virus (WSSV) in crayfish Procambarus clarkii was investigated. WSSV envelope proteins VP19 and VP28 were expressed in yeast Pichia pastoris GS115. The purified recombinant proteins (2 µg/g of crayfish) were injected intramuscularly, and the same dose injected as a booster shot on fifth day after vaccination. The vaccinated crayfish were divided into two even groups and later challenged orally by WSSV-infected dead crayfish muscle (2 g/individual) on the third and 21st days after the booster shot. The relative percent survival (RPS) in the third-day group was the highest in VP28 (91%), followed by VP19 + VP28 (84%), and VP19 (45%). The RPS for the 21st-day group was the highest in VP28 (78%), followed by VP19 + VP28 (76%), and VP19 (17%). Development of vaccine by using recombinant proteins VP19 and VP28 expressed in yeast is feasible.     

海南龙血树查尔酮合酶基因(DcCHS)的克隆及表达分析

利用RT-PCR和RACE技术对海南龙血树查尔酮合酶基因进行克隆,得到1条1 456 bp的cDNA序列,命名为DcCHS。DcCHS含有1 173 bp的阅读框架、99 bp的5′非编码区和184 bp的3′非编码区,编码390个氨基酸。DcCHS与其他植物的CHS氨基酸序列同源性高达84%以上,具有高度保守的CHS_like结构域、活性位点以及信号序列。推测DcCHS的分子量为42.7 ku,等电点pI为6.14,稳定性极差,具有15个磷酸化位点,无跨膜结构,无信号肽,亚细胞定位在细胞质的可能性较大,并预测了蛋白质的二级、三级结构。组织特异性分析结果表明,DcCHS在花中的表达远远高于根、茎、叶和果实。