山莨菪碱提高嗜水气单胞菌灭活疫苗浸泡免疫鲫的效果

为研究山莨菪碱作为佐剂在细菌灭活疫苗浸泡免疫中的作用,将山莨菪碱和嗜水气单胞菌全菌灭活疫苗联合浸泡免疫异育银鲫,首次浸泡免疫7d后加强免疫1次.通过实时荧光定量PCR检测第2、4、7、11、14和21天脾脏中IgM、IL-1β、C3、C-凝集素以及溶菌酶的mRNA表达量,并在第21天进行同源菌株活菌攻击实验.结果显示,山莨菪碱组第4天IgM和IL-1 β的表达量达到最高值,分别为28.3和332.7;而无佐剂疫苗组第11天IgM和IL-1β达到最高值,分别为56.1和791.8.山莨菪碱组的补体C3、C-凝集素以及溶菌酶的表达量都显著高于无佐剂组和对照组,且表达持续时间长.活菌攻击实验表明山莨菪碱组的相对免疫保护率可达80.0％,显著高于无佐剂组的56.0％.结果表明,山莨菪碱与嗜水气单胞菌灭活疫苗共同浸泡免疫银鲫,可以增强脾脏中IgM、IL-1β、C3、C-凝集素以及溶菌酶基因表达,提高银鲫相对免疫保护率.     

优质抗寒、抗病酿造葡萄新品种‘北红’

 ‘北红’葡萄新品种由‘玫瑰香’与‘山葡萄’杂交育成。浆果在北京地区9月底成熟。果粒圆形,蓝黑色,果粒质量1.57g,果穗质量160.0 g,浆果可溶性固形物含量23.8 %～ 27.0 %,含酸量0.89～1.26 %。早果性及丰产性强。抗寒、抗病能力特强。酿成的酒深宝石红色,酒体平衡,酒质上等。     

春秋大棚茄子专用新品种‘茄杂6号’

 ‘茄杂6号’是由自交系‘134’与‘圆杂-黑扁-1-1-M’配制而成的春秋大棚茄子专用品种。该品种耐弱光,易坐果,着色好,抗性强。株形紧凑,门茄节位8节左右。果实扁圆形,果色黑亮,果肉浅绿,单果质量850~950g,商品性好。产量一般为90~105 t·hm^-2。     

桃果实非酸/酸性状SCAR标记的转化与验证

 根据筛选到的与桃果实非酸/酸性状紧密连锁的3个AFLP标记特异片段序列信息, 设计特异引物BFPA1/A2、BFPB1/B2和BFPC1/C2。3个特异引物在‘京玉’桃、‘美味’油桃及其正反交F¹代69株群体中的PCR检测结果表明: 引物BFPB1/B2在果实性状表现为酸的后代个体中扩增出分子量大小为158 bp的特异条带, 且根据特异片段AT-CTA₁₉₉不同部位设计的引物都能稳定扩增, 只是片段大小有所差异;特异扩增检测F¹群体分离结果与原先AFLP_AT/CTA标记完全一致, 表明AFLP标记已成功转化为SCAR标记,该SCAR BFPB1/B2标记与D基因的连锁距离为2199 cM。利用SCAR BFPB1/B2标记对‘大久保’桃ב兴津’油桃的F²群体60株个体的果实非酸/酸性状进行检测, 基因型与表型性状符合率为96.7% , 并且表现为共显性标记。特异引物BFPA1/A2和BFPC1/C2的扩增多态性条带在亲本及后代中消失。     

菠萝锌指蛋白基因AcRCHY1的克隆与表达分析

 根据植物C3HC4型兼CHY型锌指蛋白的功能保守区设计简并引物, 通过RT2PCR结合RACE 方法从菠萝(Ananas comosus L. Merr) 幼苗中克隆获得了一个新的菠萝锌指蛋白基因cDNA全长, 将其命名为AcRCHY1。该基因cDNA全长1 261 bp, 开放阅读框ORF为918 bp, 推测其编码一个含有306个氨基酸残基的多肽。AcRCHY1蛋白具有保守的C3HC4 (RING finger) 和CHY两个锌指结构域, 与其它植物锌指蛋白的同源性高达81%～91%。半定量RT-PCR分析表明, AcRCHY1 在菠萝中呈组成性表达, 在子房、花瓣和小花中的表达量明显高于根和叶; 低温、高盐、干旱和ABA等非生物胁迫处理后, AcRCHY1在叶片中的表达明显增强。因此, AcRCHY1蛋白可能与花器官生长发育的调控有关, 而且可能作为一个转录调控因子在菠萝响应低温、高盐和渗透胁迫过程中参与了依赖ABA的信号转导途径。     

Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

银杏叶对辣椒植株发酵液自毒作用的化感调控

采用1/2霍格兰（Hoagland）营养液水培法,研究辣椒植株发酵液的自毒作用及银杏叶发酵液和 再腐解液对其的调控作用。结果表明,辣椒植株发酵液使辣椒受到自毒作用,根系活力下降,相对电导 率增大。银杏叶发酵液加重了辣椒植株发酵液的自毒作用,并且与其浓度成正相关。银杏叶再腐解液缓 解了辣椒的自毒作用,缓解效果与再腐解液的浓度有关。     

新疆野苹果群体遗传结构和遗传多样性的SRAP分析

 采用SRAP标记,对中国新疆野苹果4个种下居群的群体遗传结构和遗传多样性进行了研究。结果表明：10对SRAP引物总共扩增了209条带,其中206条是多态性带（98.56%）。巩留群体、新源群体、霍城群体和裕民群体分别扩增了180、169、178和165条多态性带,巩留群体的随机交配杂合度（hs = 0.3037 ± 0.0058）最高,其次为霍城群体。UPGMA聚类分析和群体间遗传分化系数显示,巩留群体和新源群体之间,以及霍城群体和裕民群体之间,遗传关系最近,群体间遗传变异最低。新疆野苹果群体内遗传变异高于群体间,占总变异的87.9%,主坐标轴分析显示4个群体是相对独立,其中巩留群体和新源群体,霍城群体和裕民群体之间,有较高的基因交流。所有参数分析表明,巩留群体遗传多样性最丰富,故在制定新疆野苹果原地和异地种质保护计划时应优先考虑巩留群体。     

Abnormalities of miR-21 expression in human breast cancer is associated with distinctive pathologic features and shortened postoperative survival

AIM: To investigate the potential relevance of miR-21 expression level to clinicopathological characteristics and patient survival. METHODS: 113 BRCA cases with more then 5 years fallow-up data were selected. Total RNA from formalin-fixed paraffin-embedded (FFPE) tissues of 113 breast cancer (BRCA) and normal adjacent tissues (NATs) were isolated for miR-21 quantitative analysis by real-time RT-PCR. RESULTS: The miR-21 expression levels in BRCA were significantly higher than those in NATs (P<0.01) with average up-regulated level of 1.74 ± 0.48. Interestingly, high level expression of miR-21 was significantly correlated with advanced clinical stage (P<0.01), lymph node metastasis (P<0.01), and shorter survival of the patients ［hazard ratio (HR)=5.476, P<0.01］. Multivariate Cox regression analysis revealed that miR-21 was one of independent prognostic impacts (HR=4.133, P<0.01) on BRCA. CONCLUSION: Over-expression of miR-21 is associated with poor prognosis of BRCA and may serve as an independent prognostic marker for BRCA.