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11.
Yufei Guo Chanjuan Shen Anchun Cheng Mingshu Wang Na Zhang Shun Chen Yi Zhou 《Veterinary microbiology》2009,138(3-4):258-265
Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis. 相似文献
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周忠义 《畜牧兽医科学(电子版)》2021,(4):108-109
草原是内蒙古地区草食畜牧养殖产业健康发展的基础,同时也是优质牧草的主要来源。有毒有害牧草生长扩散预示着草场退化,对当地草食畜牧养殖产业健康发展会造成毁灭性打击。有毒有害植物生长发育,不仅会危害牲畜健康且还会严重消耗土壤的水分和养分,妨碍优良牧草繁殖生长,使草场产量和质量显著下降。在长时间草场利用过程中,很多农牧民群众只注重索取,不注重生态环境保护,使草场生产能力和抵抗能力逐渐变差,给有毒有害牧草繁殖生长提供了条件。因此需要加强寒冷地区草原有毒有害牧草的监测,并采取针对性措施进行防控,降低发病率。该文主要探讨内蒙古呼伦贝尔市寒冷地区草原有毒有害牧草的种类,并提出相应的防治措施。 相似文献
15.
Wang G Song T Yu Y Liu Y Shi W Wang S Rong F Dong J Liu H Cai X Zhou EM 《Veterinary immunology and immunopathology》2011,142(3-4):170-178
Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status. 相似文献
16.
本研究将高作镇作为实征对象,充分围绕高作镇实际情况,提出几点农业产业扶贫模
式,并总结了高作镇农业产业扶贫的成果,旨在希望通过本研究发挥抛砖引玉的作用。 相似文献
17.
Effects of temperature and humidity on the contact angle of pesticide droplets on rice leaf surfaces
Jiantao Zhang Tengyuan Zhou Jiajun Zeng Xuanchun Yin Yubin Lan Sheng Wen 《Journal of Pesticide Science》2022,47(2):59
The effects of external factors such as temperature, humidity, pesticide formulation, and pesticide concentration on the contact angle of pesticide droplets on rice leaf surfaces were analyzed. The experiments showed that there were significant differences in the contact angles of droplets on the leaf surfaces under different temperatures and humidity. As the ambient temperature increased, the contact angle first decreased and then increased, reaching a minimum value at 25°C. With a gradual increase in humidity, the contact angle significantly increased and reached a maximum at 100% humidity. Finally, it was concluded that both the formulation and concentration of the pesticide had a significant effect on the contact angle of droplets on rice leaf surfaces. The experiments also illustrated that the effects of the pesticide formulation and concentration on the contact angle were more significant than those of temperature and humidity. 相似文献
18.
柑桔黑斑病(Citrus Black Spot,CBS)是一种真菌性病害,主要为害果实,并产生褐色斑点,严重时蔓延至全果,造成果实腐烂,显著影响果实的品质和外观,降低经济价值,被欧美等国列为重要的检疫性病害,近年来该病在我国一些柑桔产区已上升为主要病害。本文主要从柑桔黑斑病的病原菌、发病特点及检测方法等几个方面进行简要的概述。 相似文献
19.
为探讨近三十年来我国皱纹盘鲍养殖模式对群体遗传结构产生的影响,利用线粒体细胞色素C氧化酶亚基 (
20.
常年性变应性鼻炎手术疗法探讨 总被引:2,自引:0,他引:2
探索常年性变应性鼻炎的治疗新方法,方法选择各种非手术疗法不佳的常年性变应性鼻炎患者36例为手术组(6例伴有支气管哮喘),病程1.5-20a局麻下行双侧蝶腭,筛前神经电灼加下鼻甲部切除切;另选31例未行手术首作为对照组。结论蝶腭,筛前神经电灼加下鼻部分切除的新方法为治疗常年性变性鼻炎之有效的方法之一。 相似文献