Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

新疆高等寄生植物(二)——列当科

本文研究了新疆列当科2属23种植物,用检索表列出了它们的不同,总结了前人的工作,并对它们的分布、生境、寄主给予了描述。     

生物炭对盐碱条件下海滨锦葵根系与土壤特性的影响

为探索生物炭对盐碱条件下植物根系与土壤特性的影响,以海滨锦葵为材料,在盐碱土壤中添加不同含量的生物炭,测定植株根系活力、根系生长、土壤pH值、土壤电导率、土壤容重、土壤孔隙度等指标。结果显示:在盐碱胁迫下,添加生物炭提高了海滨锦葵根系活力,中高浓度生物炭明显促进植株根系生长,土壤pH值与电导率均降低,土壤容重呈上升趋势,而土壤孔隙度呈现下降趋势。经比较,5.0%和7.5%生物炭对盐碱条件下海滨锦葵根系发育与土壤特性的调控效果相对较好。     

用PCR检测副溶血性弧菌耐热溶血素基因

根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

对森林生态共享数据问题的初探

共享数据可以为我国经济社会可持续发展指标研究提供丰富的支撑。可持续发展指标的系统调查已经成为各级行政主管部门的经常性工作,数据的获得成为很容易的事情,并且形成惯例成为数字化产品序列,使指标的实施有了可操作的定量化方法。     

棉花多亲本杂交F_2群体产量性状的效应分析

本文对棉花聚合杂交体系中９个不同亲人配制的８个单交、１０个４交、１１个８交的Ｆ＿２代群体的产量及产量组合性状进行综合分析。结果表明，随着率交亲本数的增加，聚合程度逐步提高，使产量及主要产量组分的遗传增益不断上升，并达到了显著或极显著水平的增加，其中尤以８交群体的聚合增效最为突出。说明多亲本聚合杂交在棉花丰产、优质、抗病和早熟目标的育种中，对产量性状的提高具有显著效果。     

寒冷地区草原有毒有害牧草监测与防治

草原是内蒙古地区草食畜牧养殖产业健康发展的基础,同时也是优质牧草的主要来源。有毒有害牧草生长扩散预示着草场退化,对当地草食畜牧养殖产业健康发展会造成毁灭性打击。有毒有害植物生长发育,不仅会危害牲畜健康且还会严重消耗土壤的水分和养分,妨碍优良牧草繁殖生长,使草场产量和质量显著下降。在长时间草场利用过程中,很多农牧民群众只注重索取,不注重生态环境保护,使草场生产能力和抵抗能力逐渐变差,给有毒有害牧草繁殖生长提供了条件。因此需要加强寒冷地区草原有毒有害牧草的监测,并采取针对性措施进行防控,降低发病率。该文主要探讨内蒙古呼伦贝尔市寒冷地区草原有毒有害牧草的种类,并提出相应的防治措施。     

Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status.     

旋耕机刀片的耐磨性研究

结合旋耕机刀片的工况条件,运用摩擦学的研究成果,揭示了旋耕刀的磨损机理,并建立了冲蚀磨损模型。经试验验证,提出了影响旋耕刀耐磨性的因素,指出低碳高合金钢作为旋耕刀材料具有比高碳钢65Mn更好的抗磨损机能。