国外一种最新速度系数资料的插值求解

速度系数法是确定叶轮几何参数的一种常用方法。本文作者以数值分析方法求解了国外一种最新速度系数曲线资料的插值函数．从而为叶轮设计人员提供了一项方便利用国外先进技术．提高叶轮设计质量的有效工具。     

Progress in RNA-binding protein HuR and its roles in development of hepatocellular carcinoma

RNA-binding proteins （RBP） are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision （ELAV） protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma （HCC） are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC.     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.     

不同时期增补LED蓝光对番茄植株生长、果实品质和养分吸收的影响

以Micro-Tom番茄为试材,在营养液水培条件下,对果实发育成熟期补光(B)、果实发育期补光(B1)、果实成熟期补光(B2)下番茄植株生长、叶片光合参数、果实质量、果实品质、根系形态、矿物质离子平均吸收速率及其相互关系进行了研究,以期明确增补蓝光对番茄生长、果实品质、养分吸收的调控效果.结果 表明:B1处理能显著促进番茄的生长与果实膨大,增加果数、提高果实质量,与B处理的效果一致;B处理显著提高了番茄根系生长各项指标,B1处理显著促进主根的伸长,而B2处理对番茄根系生长无显著影响;B1处理或者B2处理显著提高了番茄果实中的有机酸含量,可溶性糖、维生素C含量、糖酸比与不补光处理差异不显著;B1与B补光处理显著提高了NO3-N、PO4-P、K+、Ca2+、Mg2+平均吸收速率;B1与B补光处理通过促进PO4-P与K+吸收,间接促进坐果,加快番茄果实发育,促进成熟与果实着色.因此,B1补光处理能达到与B补光处理相同的效果,优于B2处理.在低耗能补光策略上可以考虑采用果实发育期补光.     

海南西瓜蔓枯病病原菌的鉴定及8种杀菌剂对其室内毒力测定

对海南西瓜蔓枯病病原菌进行了分离鉴定及室内杀菌剂筛选,为西瓜蔓枯病的防控提供参考依据.采用PDA培养基,对西瓜病株病原菌进行分离、培养,经致病性测定,形态学分析及rDNA-ITS測序,鉴定其致病菌为Stagonosporopsis cucurbitacearum.选用8种不同类型杀菌剂对西瓜蔓枯病菌进行室内毒力测定,结果表明,8种杀菌剂均对西瓜蔓枯病菌有一定的抑制作用,其中24％双胍.吡唑酯可湿性粉剂(WP)和11％精甲.咯.嘧菌悬浮种衣剂(FSC)的毒力较强,EC50值分别为0.101 7、0.391 1 mg/L,均小于1 mg/L;其次是10％苯醚甲环唑水分散粒剂(WDG)和18.7％丙环.嘧菌酯悬浮剂(SC),EC50值分别为1.393 3、3.037 6 mg/L;27％春雷?溴菌腈WP和22.5％啶氧菌酯SC毒力一般,EC50值分别为10.753 0、20.982 6 mg/L;毒力效果较差的是46％氢氧化铜WDG和50％多菌灵WP,EC50值分别为60.703 9、151.477 0 mg/L.     

广西蚕区主推家蚕品种在不同区域及不同批次饲养的适应性调查

将广西壮族自治区主推的3对家蚕品种分别在桂西北、桂中和桂南几个具有地域代表性蚕区的不同养蚕批次进行适应性饲养试验,调查不同品种的蚕茧产量和品质,以期通过家蚕品种最好的生产应用布局来提高广大蚕农的经济收益.根据试验结果建议:那坡、环江蚕区每年第1、2、3、10、11批次对饲养家蚕品种的选择顺序为桂蚕2号、两广二号或桂蚕N...     

刈割次数对苜蓿相关表型性状与饲草产量的影响

试验选取4份苜蓿材料,设4次刈割处理,观察测试表型性状及干物质的变化动态,并用DPS7.05数据处理系统对各性状指标与饲草产量的关系进行计算分析,以探讨刈割次数对苜蓿表型性状及饲草产量的影响,为高效优质生产提供依据.结果表明,刈割频度显著影响苜蓿越冬率(P<0.05),苜蓿株高、茎粗、茎叶比、分枝数等指标均随刈割次数增...     

两系杂交粳稻产量性状优势形成分析

采用程氏指数对两系杂交组合亲本及其杂种F1进行分类,对杂种F1产量性状与亲本及杂种F1程氏指数,杂种F1产量性状与父本产量性状进行相关分析。结果表明,偏粳×粳、偏粳×偏粳的组合杂种F1为偏粳类型。杂种F1每穗总粒数表现与双亲程氏指数差呈显著正相关,同时也受到父本性状值的影响。     

不同播期夏播小豆产量性能动态指标与光温水效应

为探讨光、温、水等气象因子对夏播小豆产量的影响,达到合理利用气象资源、进一步挖掘小豆增产潜力的目的,以中晚熟小豆品种花小豆(V1)和中早熟品系德红5261(V2)为材料,设T1(6月17日)、T2(6月22日)、T3(6月27日)、T4(7月2日)和T5(7月7日)5个播期,形成不同的光、温、水生长环境,对夏播小豆产量...     

中国水肥一体化施肥设备研究现状与发展趋势

为了促进中国水肥一体化施肥设备的研究开发及推广应用,总结分析了中国水肥一体化施肥设备科研、生产及实际应用中存在的问题,概述了施肥设备的发展现状,重点阐述分析了文丘里施肥器、压差施肥罐、比例施肥泵、柱塞泵及固体肥料溶解施肥装置等常用施肥设备的技术特点、研究进展和存在问题等.在此基础上,基于绿色农业发展理念和农业物联网的发...