‘小黄’菊遗传转化再生体系的建立

以‘小黄’菊茎段、叶盘为外植体 ,选用MS培养基为基本培养基 ,附加激素 6 BA和NAA ,通过研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响 ,建立了较好的遗传转化再生体系 .试验结果表明‘小黄’菊茎段、叶盘最适分化培养基分别为MS +6 BA 2mg L +NAA 1mg L和MS +6 BA 1mg L +NAA 0 1mg L ;小苗的最佳生根培养基为 1 2MS +NAA 0 1mg L ,生根率可达 10 0 % ;4 0mg L卡那霉素可以抑制茎段的分化 ,10mg L卡那霉素可以抑制叶片的分化 ,2 0mg L卡那霉素可以抑制分化小苗的生根 .     

小麦灌浆后期青枯骤死原因分析及控制

小麦灌浆后期植株衰老与活性氧自由基毒害和膜脂质过氧化密切相关。灌浆后期株体内活必砼谢平衡失调是青枯发生的主要内因：造成青枯的外因除与“Ｖ”型变温有关外，雨后骤晴的强光与高温协同作用亦是青枯发生的重要外因一。灌浆后期“Ｖ”型变温使麦株各器均受到伤害，尤其穗下茎受到伤害。青枯能否发生取决于植体内酶活力的高低、非酶清除剂含量的多少以及“Ｖ”型变温的强度和持续时间。选择优良品种、适时播种、合理施肥和加强     

鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析(英文)

Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity (99.9%) was observed between the isolated strain and one of the strains of Gallibacterium anatis (AF228002), the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI (AF228016, Gallibacterium genomosp.1, AF228017, Gallibacterium genomosp.2, AF228018, Gallibacterium genomosp.1) were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7%-93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis.     

新育成反向核不育水稻FHS开花习性观察(英文)

[Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 and the mutant FHS with special fertility that was isolated from their offspring. Some characteristics such as flowering habit and stigma exsertion rate of FHS were observed in this experiment. [Result] The reverse genic sterile rice FHS had an obvious peak flowering stage from 10:00 to 10:30, while the second peak flowering was from August 5 to August 10. Compared with PA64S, FHS flowered early and its flowering time was concentrated, showing that it is for seed propagation. The stigma exsertion rate of FHS was 85.8% and low exsertion rate was good for the purity of seed. [Conclusion] The reverse genic sterile rice FHS had good value in use, besides, it could also be used as comparison material for studying fertility alternation mechanism of photoperiod sensitive genic male sterile rice.     

种植密度对夏玉米穗位叶片解剖结构的影响

为了研究种植密度对玉米穗位叶片解剖结构的影响, 以生产上广泛种植的玉米杂交种郑单958和浚单20为材料,采用石蜡制片和超薄切片法对不同种植密度下2个玉米品种穗位叶片和叶绿体的结构进行了观察分析。结果表明, 随着种植密度的增加,2个玉米品种的叶片厚度、叶脉横截面积和叶脉木质部面积减小,上、下表皮的气孔频度减少(郑单958显著,浚单20不显著),叶肉细胞叶绿体的基粒数量减少,维管束鞘细胞叶绿体的数量减少;种植密度在75 000株 hm^-2时产量最高。相关性分析显示,叶脉木质部面积、气孔频度、叶肉细胞叶绿体的基粒数量、叶肉细胞叶绿体的基粒数量与种植密度呈显著负相关,与单穗籽干重、千粒重、穗粒数量呈显著正相关,因而成为玉米生产上的重要结构指标。     

东方蝾螈、太行隆肛蛙、丽纹龙蜥心脏的比较组织学研究

采用H．E．染色、显微镜观察法对东方蝾螈、太行隆肛蛙、丽纹龙蜥的心脏进行研究．结果显示：东方蝾螈、太行隆肛蛙心脏结构基本相同,丽纹龙蜥的心脏与二者有一定差别．3种动物的心脏均为两心房一心室,但丽纹龙蜥的心室开始出现不完全分隔,为不完全的双循环系统;红细胞均为有核红细胞;心脏壁有内、中、外3层结构,分别为心内膜、心肌层及心外膜．心内膜位于心脏内层,表面是一层不规则的多角形内皮细胞,胞核为椭圆形．心肌层主要由心肌纤维构成,心肌纤维间充满结缔组织和血管．心肌纤维由心肌细胞组成,胞核呈圆形或椭圆形,位于细胞中央．心外膜位于外层,由结缔组织构成,内含较多弹性纤维．3种动物心脏结构的差别是两栖类、爬行类动物在进化过程中对环境不断适应的结果．     

AMMI模型在芝麻区域试验中的应用

运用AMMI模型对2000年河南省芝麻区域试验数据进行分析。结果表明，AMMI模型能具体、直观、灵敏地综合评价芝麻区域试验中参试品种的稳定性。同时还分析了Di(1)与Di(2)的一致性问题，并对基因与环境的互作进行了探讨。     

鲁山牛腿山羊体尺性状与微卫星标记的相关分析

根据比较基因组学和相关文献资料,选择与山羊和牛生长性状相关的6个微卫星基因座(BM6444,MAF70,BM315,BMS1678,BM1818和BMC1206)作为分子标记,研究了牛腿山羊的群体遗传变异,并分析了体尺性状与6个微卫星标记之间的关系.结果表明:6个微卫星标记在牛腿山羊群体中的多态信息含量(PIC)分别为0.887,0.863,0.773,0.840,0.888和0.791,遗传杂合度(He)分别为0.904,0.883,0.808,0.864,0.904和0.824,有效等位基因数(Ne)分别为9.62,8.05,5.02,6.96,9.68和5.46.6个微卫星标记与体尺性状的最小二乘分析结果表明:BM6444基因座165bp和145bp等位基因对体质量、体高、体长、胸围和胸深有正效应,而154bp和137bp等位基因对体高、胸围和胸深性状有负效应;MAF70基因座180bp和159bp等位基因对体质量性状有正效应,159bp和144bp等位基因对体高、胸围和胸深有负效应;BM315基因座140bp等位基因对体高有正效应,而136bp等位基因对胸深有负效应;BM1818基因座298bp和27...     

密集烘烤过程中烤房叶间风速指标的灰色关联聚类分析

在烘烤过程中,密集烤房内部不同位点的风速之间密切又复杂.为确定风速指标间的关系,测定了密集烤房内405个风速值,对15个风速指标进行了灰色关联聚类分析.结果表明:当聚类临界值为0.8时,15个风速指标可被聚为2类;当聚类临界值为0.7时,15个风速指标可被聚为1类.通过分析,密集烤房内部同一平面不同位点的风速差异较小,可视为均匀一致,密集烤房能够保证装烟室同一层烟叶整体上均处于同样的变色和干燥环境下,从而能够在理论上保证其烟叶相同的烘烤质量.     

提高粮食综合生产能力的技术路径——以河南省为例(英文)

[目的]根据河南省情和生产实际,探讨提高粮食生产能力的技术路径。[方法]对河南省粮食产量的现状与动态进行整体分析。[结果]河南省粮食总产量的变化呈现上升态势。2010年粮食总产5437万t是1978年粮食总产2097.40万t的2.5倍。粮食总产的增长越来越依赖于单产的提高。[结论]该研究为河南省乃至全国粮食增产提供理论依据。