Effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS Thr495 phosphorylation in pulmonary artery endothelial cells

AIM: To examine the effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS activity in pulmonary artery endothelial cells (PAECs).METHODS: 1.Rat pulmonary artery (PA) rings of intrapulmonary arteries were suspended under isometric tension in oxygenated Krebs buffer in order to observe the differences on constriction induced by 15-HETE using nitro-L-arginine methyl ester (L-NAME, 10-4 mol/L, an inhibitor of eNOS) and removal of endothelium.2.The bovine PAECs was cultured for NO assay by Greiss reaction.3.Immunoprecipitation (IP) and Western blotting (WB) were used to analyze the cell extracts from bovine PAECs treated with 2×10-6 mol/L 15-HETE for 30 and 60 min, using phosphatase-eNOS (Thr495) antibody for IP and eNOS antibody for WB.RESULTS: 1.Inhibition of eNOS with L-NAME and removal of endothelium significantly enhanced 15-HETE induced contractions in rat PA rings in a concentration-dependent manner (P＜0.05, P＜0.01).2.15-HETE increased phospho-eNOS (Thr495) levels (P＜0.01) in cultured bovine PAECs.3.10-6 mol/L 15-HETE (P＜0.05) did significantly reduce nitrite production, whereas 10-5 mol/L cinnamy l 3, 4-dihydroxy-［alpha］-cyanocinnamate (CDC, P＜0.05), 10-4 mol/L nordihydroguiairetic acid (NDGA, P＜0.01) significantly increased nitrite production, as measured by a spectrophotometric assay.CONCLUSION: 15-HETE inhibits eNOS activity and decreases production of NO (NO-2/NO-3).The pathway of eNOS/NO is involved in 15-HETE-induced contraction in the rat PA.     

胶体染料试纸条法快速诊断家畜血吸虫病的研究

为了适应现场家畜血吸虫病的筛查需要,设计了简便快速的胶体染料试纸条诊断技术用于检测家畜血清中的抗血吸虫抗体,以该方法检测血吸虫病牛、猪血清83份,其结果为阳性检出率100%,健康牛、猪血清73份的检测结果为阴性符合率分别为95.3%和94.0%,与感染肝片形吸虫的牛、羊血清16份的交叉反应率为18.8%.表明本方法敏感性高,特异性较强.     

谈谈高校图书馆现采工作

从图书馆现场采购工作的优缺点入手，阐述了提高高校图书馆采购质量、不断完善现采工作必须从加强图书馆采购队伍建设、选择现采地点及现采工作中应注意的事项等方面着手。     

Intranet信息安全工作中客户端的防护

本文从内网客户端的软件、硬件和安全意识几个方面阐述了Intranet信息安全工作的重心和防护措施。     

黑茶镳子扦插育苗技术研究

对3个品种黑茶镳子‘坠玉’‘寒丰’‘绥研’的扦插技术研究表明,以当年生枝条为穗材,‘坠玉’用ABT 500 mg/L速蘸,生根率达43.3%,生根数达8~10条;‘寒丰’用ABT 200 mg/L或IBA 200 mg/L浸泡3 h,扦插生根率达100%,生根数大于30条;‘绥研’用ABT 500 mg/L速蘸3~5 s,扦插生根率达83.3%,生根数达8~10条。     

早熟高产优质抗病大豆新品种黑河19特征特性

黑河19大豆早熟、高产稳产、优质、抗病、秆强、喜肥水、不炸荚、适应性广，一般产量2488～2861kg／hm^2，增产幅度10．02％～13．90％，高产攻关或窄行密植可达3750kg／hm^2，2002～2004连续3年累计推广面积81万hm^2，增产大豆2．2亿kg。     

Sulphate Attack Resistance of the Concrete with Additives and Its Assessment

The influence of three kinds of additives: silica fume,slag and fly ash on the sulphate attack resistance of concrete were studied.The experiment results show that these three kinds of additives can improve sulphate attack resistance of the concrete and the order of their efficiency from high to low is silica fume,slag and fly ash;the development of mortar expansion value is closely related to the degree of sulphate attack resistance of the concrete;integrated assessing two concrete properties: mortar expansion value and compressive strength ratio can more exactly assess the sulphate attack resistance of the concrete.     

优质和抗稻瘟病的水稻种质资源筛选

测定了40份水稻种质资源对稻瘟病的抗性、碾米及外观品质。结果表明，40份品种高抗至中抗稻瘟病，抗病频率为40.0%～100.0%，其中20份达80%以上。40份品种对优势小种群ZB群平均抗病频率为80.0%，对重要小种群ZA群的平均抗病频率为70.2％，对ZC群的平均抗病频率为80.5%。从中筛选出24份碾米品质和外观品质较优的抗稻瘟病种质材料，其出糙率76.1%～80.9%，精米率68.7%～72.6%，整精米率35.7%～64.3%，垩白粒率5%～30%，垩白度1.2%～12.7%，长宽比2.7～3.6，其中6份的碾米品质和外观品质达国家三级优质标准，4份达国家二级优质标准。这些优质抗源材料对选育高产、优质、抗稻瘟病新品种（组合）具有重要利用价值。     

海岛棉零式果枝与长果枝品种间杂交F1目标产量性状的决策分析

采用MINQUE（1）统计方法，利用AD模型对9个海岛棉品种（系）及其20个F1组合产量性状的两年资料进行了相关分析和目标产量性状的决策分析。结果表明，不同性状间遗传组分相关系数的大小顺序并不能代替其他性状对霜前皮棉产量和皮棉总产决策系数的大小顺序。还确定了提高海岛棉零式果枝与长果枝品种间杂交杂种后代霜前皮棉产量     

A Study on Urban Land Structure Optimization Model

As far as the urban land structure is mentioned, it means the distribution of urban land among the different industries of the city. The problem of urban land structure is referred as unreasonable allocation of urban land caused by several factors, including the decision making mechanism of old economy and urban development policies. Different from using Fractal theory, Entropy theory and Functional theory, in this paper, with reference to Dissipative Structure Theory, one dynamic, systematic and historical optimization model of Max f(J_i, X_i) with two parameters of J_i and X_i will mainly be set up. This model can be used for analyzing the key factors determining the optimization of urban land structure.