控光增绒技术在辽宁绒山羊中应用效果观察

2010～2012年,辽宁省畜牧科学研究院先后在本院科技示范场、凤城市绒山羊养殖户,开展了辽宁绒山羊控光增绒试验,设置了不同控光时间的试验组以及相应的对照组,以期验证该项技术在辽宁地区和辽宁绒山羊生产中的应用效果。试验结果表明,该项技术对辽宁绒山羊增绒效果不明显,达不到一年两次剪绒、提高产绒量的目的,建议谨慎将该项技术应用于辽宁地区辽宁绒山羊生产中。     

西杂牛连续埋植“畜大壮”效果观察

本试验对甘肃河西地区西杂１－２岁和周岁以下的犊牛连续用促生长剂“畜大壮”分阶段进行耳根埋植,试验结果表明,４－６月龄犊牛全期１８０天,试验组增重和平均日增重分别为５．５ｋｇ和３０８．３３ｇ,试验组比对照组提高３７．２５ｋｇ和２０６．９４ｇ（Ｐ〈０．０１）,１－２岁牛全期１８０天,试验组平均增重和日增重分别为５６．４３ｋｇ和３１４．４９ｇ,试验缄比对照组分别提高３０．０３ｋｇ和１６６．８２ｇ差异显著     

加强生产现场管理把浪费扫地出门

作为一名多年从事饲料企业经营的管理者,对饲料企业的生产现场管理感受很深,在笔者考察过的几十家饲料企业(也有外企)中,现场管理或多或少都存在着问题,有的问题甚至相当得突出。或许我们的管理者没有引起重视,或许是我们的制度执行不到位,不管是什么原因,产品的     

Marek's disease resistant/susceptible MHC haplotypes in Xiayan chickens identified on the basis of BLB2 PCR-RFLP and BLB2/BF2 sequence analyses

1. The aim was to analyse the variability of the BLB2/BF2 genes of Xiayan chickens to identify homozygous birds with resistance or susceptibility to Marek's disease (MD).

2. The experiment used two lines: birds from a common line were divided into Group A (unvaccinated) and Group B (vaccinated with herpesvirus of turkeys (HVT)); and birds from an MD-resistant line were divided into Group C (unvaccinated) and Group D (vaccinated with HVT). They were challenged intra-abdominally with Marek's disease virus (MDV) and genomic DNA extracted from peripheral blood lymphocytes so that polymorphism of the BLB2/BF2 genes could be analysed using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and nucleotide sequence analysis.

3. A 374-bp fragment of the BLB2 gene was amplified from the samples and, after digesting with restriction enzymes Alu I, Cai I, Cfr I, Hin1 I, Hinf I and Rsa I for RFLP analysis, the 6 electrophoretic patterns were analysed. Seven homozygous genotypes were found and used tentatively to identify alleles of the BLB2 gene.

4. A 765-bp fragment of the BF2 gene was amplified from the 7 samples for cloning and sequencing.

5. Six homozygous birds were confirmed from the sequenced BLB2/BF2 gene. Four birds were resistant to MD. Three birds had identical nucleotide sequences and were highly homologous with MHC haplotype B⁶, which is MD resistant. One bird had high homology with the highly MD-resistant B²¹ haplotype, and two birds were susceptible and highly homologous to the B¹⁹ haplotype, which is highly MD susceptible.     

HACCP体系在出口兔肉检验检疫备案养殖场中的应用

输欧盟兔肉一直是出口敏感商品之一,多次因药物残留等问题被欧盟通报,严重影响了我国兔业的出口,而问题的根源主要在肉兔的养殖过程。本文就肉兔养殖过程中建立和应用HACCP体系的可行性和必要性进行探讨,对养殖过程中的安全危害进行分析,确定关键控制点,提出控制措施,从源头保证输欧兔肉产品的质量安全卫生。     

达乌尔黄鼠染色体组型和G带分析

本文研究了达乌尔黄鼠东北亚种的骨髓细胞染色体组型和Ｇ－带带型。确定其染色体数为２ｎ＝３６，各染色体有其特有Ｇ－带带型。     

中国荷斯坦牛乳蛋白分子遗传多态性和产奶性状相关性的研究

从北京两个主要牛场共采得１８７头中国荷斯坦奶牛的血样,提取基因组ＤＮＡ,通过ＰＣＲ－ＲＦＬＰ方法对Ｋａｐｐａ酪蛋白、Ｂｅｔａ乳球蛋白（βｌｇ）和Ａｌｐｈａ乳白蛋白（α－ｌａ）进行了基因型的鉴定,并结合产奶性状进行统计分析,结果表明,牛群中上述３种乳蛋白基因的基因频率分别为Ｋ－ｃｎＡ７９％,Ｋ－ｃａＢ２１％,β－ｌｇＡ４３％,β－ｌｇＢ５７％,α－ｌｇＢ１００％,Ｋ－ＣＮ和β－ｌｇ基因位眯对产奶量没     

实验动物房消毒试验研究

选用５种常用消毒药物进行消毒试验,抑菌试验。结果表明实验动物房选择消毒药物的顺序为：乳酸、醋酸钠、百毒杀、卫康、过氧乙酸,消毒间隔以３个月为宜。     

特高多花黑麦草在贵州的适应性研究

通过对特高多花黑麦草在贵州不同生态条件下的试验研究,结果表明:特高多花黑麦草在三个不同地区均能完成正常的生育期,且生长良好、适应性强、产草量高、营养价值丰富,具有良好的推广应用前景。     

Surveillance of Parapoxvirus Among Ruminants in Virginia and Connecticut

In 2008, two deer hunters in Virginia and Connecticut were infected with a unique strain of pseudocowpox virus, a parapoxvirus. To estimate the prevalence of this virus, and in an attempt to define the reservoir, Parapoxvirus surveillance was undertaken between November 2009 and January 2010. 125 samples from four ruminant species (cows, goat, sheep and white‐tailed deer) were collected in Virginia, and nine samples from white‐tailed deer were collected in Connecticut. We found no evidence that the parapoxvirus species that infected the deer hunters is circulating among domesticated ruminants or white‐tailed deer. However, parapoxvirus DNA of a different parapoxvirus species, bovine papular stomatitis virus (BPSV), was detected in 31 samples obtained from asymptomatic cattle in Virginia. Parapoxvirus DNA–positive cattle originated from the same counties indicating probable transmission among animals. Molecular analysis identified BPSV as the parapoxvirus affecting animals. Asymptomatic parapoxvirus infections in livestock, particularly young animals, may be common, and further investigation will inform our knowledge of virus transmission.