<Emphasis Type="Italic">Echinococcus granulosus</Emphasis>: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with r<Emphasis Type="Italic">Eg.</Emphasis>myophilin

Objects:  

This study aimed to investigate the immunoprotection of the recombinant Eg.myophilin (rEg.myophilin) and tentatively analyze mechanism of this protection.     

控光增绒技术在辽宁绒山羊中应用效果观察

2010～2012年,辽宁省畜牧科学研究院先后在本院科技示范场、凤城市绒山羊养殖户,开展了辽宁绒山羊控光增绒试验,设置了不同控光时间的试验组以及相应的对照组,以期验证该项技术在辽宁地区和辽宁绒山羊生产中的应用效果。试验结果表明,该项技术对辽宁绒山羊增绒效果不明显,达不到一年两次剪绒、提高产绒量的目的,建议谨慎将该项技术应用于辽宁地区辽宁绒山羊生产中。     

中国婴幼儿配方奶粉市场的研究进展

婴幼儿配方奶粉是根据婴幼儿生长的不同时期发育所需营养不同的特点而设计的产品,它是婴儿母乳的替代食品。并就中国婴幼儿配方乳粉的发展市场的发展历程、发展方向及对发展中国婴儿配方奶粉市场的建议进行了综述。     

High‐level exogenous trans10, cis12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells

Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75–150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not have inhibiting action on cell proliferation (P > 0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P < 0.0001) of medium chain fatty acids (MCFA, C14 < C < C16), a 26.5% reduction (P < 0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P = 0.046), fatty acid synthase (FAS) (P = 0.017) and stearoylCoA desaturase1 (SCD1) (P = 0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P = 0.020) and long chain acylCoA synthetases (ACSL) (P = 0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.     

不同土地利用方式下表层土壤大团聚体特征

土壤大团聚体含量能表征土壤结构稳定性,反映土壤结构变化趋势。本研究在辽河干流中下游流域主要沙化区分析湿地、林地、农田和草地4种土地利用方式对0-20cm土层土壤大团聚体(0.25 mm)含量和稳定性的影响,及各粒径大团聚体的空间异质性,并进行空间插值模拟其分布。结果显示,农田土壤团聚体破坏率最大,达到46.98%,林地最小,为26.36%;依据土壤水稳性大团聚体含量、团聚体颗粒平均重量直径和几何平均直径,评价土壤结构稳定性强弱为湿地林地农田草地;4种土地利用导致土壤大团聚体稳定性差异显著(P0.05),湿地和草地之间的差异性最显著;湿地土壤结构相对最好,其次是林地和农田,草地相对最差。本研究结果可为辽河干流流域土地沙化程度评价及分区治理提供参考依据。     

生地提取物对雏鸡免疫器官指数和H9疫苗免疫效果的影响

本试验旨在研究生地提取物对雏鸡免疫器官发育和H9疫苗效果的影响,采用单因素分组设计,选用1 200只1日龄雏鸡,随机分为6组,生地提取物添加组（Ⅰ、Ⅱ、Ⅲ组）分别在基础日粮中添加1‰、2‰、3‰生地提取物,黄芪多糖组（Ⅳ组）在基础日粮中添加0.4‰黄芪多糖,免疫不给药组（Ⅴ组）免疫H9疫苗并饲喂基础日粮,空白组（Ⅵ组）不免疫H9疫苗并饲喂基础日粮,药物添加期为7 d,分别在雏鸡14、21、28、35、42、49、56日龄进行采样,测定鸡群的H9抗体水平,计算法氏囊和脾脏的脏器指数。结果显示：①与Ⅵ组相比,Ⅰ、Ⅱ、Ⅲ、Ⅳ组均能显著提高雏鸡脾脏和法氏囊指数（P < 0.05）,促进雏鸡的脾脏和法氏囊的生长发育,其中,Ⅱ组（2‰生地提取物）的脾脏指数和法氏囊指数较Ⅰ、Ⅲ组显著提高（P < 0.05）,且Ⅱ组与Ⅳ组无显著差异（P > 0.05）。②黄芪多糖和生地提取物的添加可提高雏鸡血清禽流感疫苗抗体效价并且使H9抗体维持在较高水平（log2）,从42日龄起,Ⅱ组的抗体滴度显著高于Ⅰ、Ⅲ组（P < 0.05）,同时与Ⅳ组无显著差异（P > 0.05）。综上所述,生地提取物具有促进雏鸡的脾脏和法氏囊发育的作用,配合H9疫苗使用,可以维持抗体处于较高滴度,建议生地提取物在雏鸡日粮中的添加剂量以2‰为宜。     

达乌尔黄鼠染色体组型和G带分析

本文研究了达乌尔黄鼠东北亚种的骨髓细胞染色体组型和Ｇ－带带型。确定其染色体数为２ｎ＝３６，各染色体有其特有Ｇ－带带型。     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

Association of polymorphisms for nuclear receptor coactivator 1 gene with egg production traits in the maternal line of Shaobo hens

1. The objectives of the study were to find polymorphic sites and elucidate the association between SNPs in the nuclear receptor coactivator 1 (NCOA1) gene and reproductive traits. 2. SNPs were detected by PCR-SSCP and DNA sequencing. Four SNPs were detected, including T10155007A, T10125838C, G10118492A and G10109315T. Three polymorphisms were associated with total egg production at the age of 300 d and the G10109315T polymorphism was associated with age at first egg. 3. In conclusion, the NCOA1 gene can be used as a molecular marker for reproductive traits in hens.