Guinea Pig Model of Uterine Leiomyoma Established by Intragastric Administration of Estrogen

[Objective] The paper was to establish the guinea pig model of uterine leiomyoma by intragastric administration of estrogen and to dis-cuss the effect of different doses and administration cycles on formation of uterine leiomyoma. [Method] Mature female guinea pigs were divided into short term model group and long term model group, and intragastrically administrated with estradiol valerate at the dose of 0.1 mg/100 g body weight twice a week for 6 and 8 weeks respectively. Guinea pigs intragastrically administrated with equal volume of normal saline were set as con-trol. After 6 or 8 weeks, the guinea pigs were sacrificed. The serum levels of estrogen and progesterone were measured by ELISA and the leiomyoma formation rate was measured. Histological changes were compared between treatment group and control group with HE staining. [Results] The leiomyoma formation rate was 30% in short term model group and 40% in long term model group. The overall leiomyoma formation rate was 35%.The serum level of estrogen in model animals increased significantly and the progesterone level decreased in long term model group. Histopathologi-cal examination confirmed that the guinea pig model of uterine leiomyoma was established successfully. [Conclusions] Intragastric administration of estrogen is a simple and effective method for establishing guinea pigs model of uterine leiomyoma.     

瘤胃缓释氮对奶牛泌乳性能的影响

试验旨在研究瘤胃缓释氮对奶牛泌乳性能的影响。选用胎次不同、产犊日期相近、泌乳中期的中国荷斯坦奶牛62头,随机分为两组(试验组和对照组),每组31头。试验前15d为预饲期。在预饲期内,试验组添加0.5%瘤胃缓释氮替代3%豆粕;到第16d添加1%瘤胃缓释氮替代6%豆粕;第38d添加1%瘤胃缓释氮替代6%的菜粕。结果表明,与对照组相比,试验组添加瘤胃缓释氮可提高奶牛对干物质的采食量,平均产奶量提高1.20kg,平均饲养成本降低0.33元/d,经济效益增加。     

一起不履行动物强制免疫义务案的分析

通过对一起不履行动物强制免疫义务案件的分析,指出同类案件在办理过程中容易出现的问题,以及的目前执法工作中存在的一些不足,针对这些问题提出了解决建议。     

酒泉市动物产地检疫现状及对策

随着社会经济迅速发展,酒泉市动物及动物产品流通日益频繁,动物产地检疫极为重要。本文主要论述了酒泉市动物产地检疫的作用、意义、现状、存在的问题及解决对策和措施。     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

LFA—3和IL—2对机体免疫增强作用

以绵羊红细胞膜提取的ＬＦＡ－３和猪外周血单个核细胞所产生的ＩＬ－２，同时或分别与新城疫Ⅳ系疫苗胸肌注射２２日龄雏鸡，测定鸡体ＨＩ抗体效阶及Ｅｔｇ花环形成率的变化，结果表明，ＬＦＡ－３组抗体效价与对照组差异极显著，ＩＬ－２也可显著提高ＩＶ系苗免疫后ＨＩ抗体效价，且两者的作用具有剂量依赖性。     

搅拌型酸奶流变学特性的研究

以市场上流行的6种搅拌型酸奶为研究对象,对其流变学特性及质构特性进行了初步的研究.结果表明:6种酸乳的剪切应力值在升速和降速过程中都能形成触变环,表观粘度在升速过程中随剪切应力的增大而逐渐降低,降速过程中随着剪切速率的降低而升高;酸奶在16 r/min的剪切速率下,转速和相应的表观粘度符合幂率定律,进一步发现搅拌型酸奶具有时间触变性,是一种正触变性流体.由触变环的面积可知,6种搅拌型酸奶的触变性大小:S5＞S4＞S2＞S1＞S3＞S6,触变性的大小对样品的口感有非常重要的影响.此外,酸奶的流变学分析得出口感较好的酸奶粘度值应在540.56～674.25 mpa·s范围内;酸奶质构分析发现口感较好的酸奶硬度在33.9～39.9 g范围内.     

贵州省鸡淋巴性白血病J亚型血清学调查研究

为了解和掌握鸡淋巴白血病J亚型(ALV -J)在贵州的发生和流行的基本情况,在7个主要养殖地区的35个养鸡生产场共采集1 205份血清样本,采用ALV -J诊断试剂盒进行了ALV -J的ELISA血清学抗体检测.检测及相关流行病学调查结果表明:ALV -J亚群的平均阳性感染率为13.776％,其中肉鸡为20.050％,...     

铜对意大利蜜蜂工蜂生理机能的影响

本研究旨在探索意大利蜜蜂工蜂成蜂饲粮中铜的添加水平对蜜蜂生长发育、生理生化及抗氧化性能的影响。从蜂群中随机取3000只刚出房的蜜蜂,随机平均分成6组,分别命名为A、B、C、D、E、F,每组5个重复,每个重复100只蜜蜂;6组工蜂幼虫分别饲喂铜的添加水平为0.00、2.00、4.00、6.00、8.00、10.00μg/g的糖水,饲养至全部死亡。分别取成蜂测定体成分、血淋巴生化指标、抗氧化指标、相关基因表达量、制作中肠切片,并测定寿命。结果表明:(1)铜对蜜蜂寿命的影响显著,从蜜蜂存活率曲线图来看,当饲粮中铜的添加水平为2.00μg/g时对蜜蜂中肠食膜的结构最为有利。(2)与对照组相比,铜的添加水平为2.00~10.00μg/g组9日龄蜜蜂体组织铜含量显著升高(P<0.05),蜜蜂血淋巴中总蛋白(TP)和总胆固醇(TCHO)含量显著降低(P<0.05)。(3)9日龄蜜蜂蜂体超氧化物歧化酶(Cu/Zn-SOD)活性随着铜添加水平的提高出现先升高后降低的趋势,在2.00μg/g时活性最高(P<0.05)。(4)当饲粮中铜的添加水平在0.00~10.00μg/g的范围内不断升高时,CAT、Ctr1基因相对表达量显著增加(P<0.05)。由此得出饲粮中铜的添加水平对蜜蜂的生理机能影响显著。