Polyaniline-Modified Quartz Crystal Microbalance Sensor for Detection of Formic Acid Gas

A polyaniline-modified quartz crystal microbalance (QCM) sensor was obtained through immobilizing the polyaniline film on the silver electrode surface of quartz crystal resonator by an electrochemical method. The sensor was studied for detecting the formic acid gas of different concentrations, and the results showed that the resonant frequency of QCM decreased quickly in the beginning and tended to be constant in the end when exposed to formic acid gas. The frequency shifts decreased faster as the concentration of formic acid gas increased. And the frequency shifts of the QCM sensors were found to be linearly related to the concentration of formic acid gas, which might be used to estimate the concentration level of the formic acid gas within the range of experimental concentrations. The result of on-line monitoring test fully indicated that the QCM sensor responded effectively to the increasing concentration of formic acid and had important practical significance and broad application prospect in real-time detection of antique conservation environment in the museum.     

Effects of cysteamine on mRNA levels of growth hormone and its receptors and growth in orange-spotted grouper (Epinephelus coioides)

Effects of cysteamine (CS) on growth hormone (GH) mRNA, two types of growth hormone receptor (GHR) mRNAs and growth rate in orange-spotted grouper (Epinephelus coioides) were investigated. CS could cause a modification in the structure of somatostatin, which is the most important neuroendocrine inhibitor of basal and stimulated growth hormone synthesis and release, and renders it nonimmunoreactive probably through interaction with the disulfide bonds. In the present study, cysteamine hydrochloride (CSH) enhanced the level of pituitary GH mRNA in a dose-dependent manner through attenuating or deleting the inhibiting action of somatostatin on GH mRNA expression. CSH at relatively low doses (from 1 to 3 mg/g diet) enhanced the levels of two types of GHR mRNAs in dose-dependent manner, whereas the stimulation induced by CSH declined from the peak at higher dose of CSH (4 mg/g diet). It might be attributed to the variation in GH-induced up-regulation of GHRs at different doses of GH. Feeding of CSH could induce remarkable enhancement of growth rate in orange-spotted grouper. In addition, the stimulatory effect of CSH could be potentiated by the additive effect of luteinizing hormone-releasing hormone analog (LHRH-A). Compared with individual treatments, combined feeding of CSH and LHRH-A caused more efficient elevation of growth rate after 8 weeks of feeding. CSH and LHRH-A individually and in combination remarkably increased the levels of GH and GHR mRNAs compared with the control. The combined administration of CSH and LHRH-A in diet was most effective to enhance the level of GH and GHR1 mRNA. The morphological characteristics of the experimental fish were evaluated. Compared with control, the ratios of muscle RNA/DNA, condition factors (CF) and feed conversion efficiency (FCE) were significantly enhanced in the treated groups, while the highest values were observed in the combined treatment. All the results suggested that CSH (1–3 mg/g diet) is an effective, economical and feasible feed additive in orange-spotted grouper culture.     

旱作大豆播种密度对产量和水分利用效率的影响

为了提高旱作区大豆种植效益,充分利用当地降水资源,在甘肃东部地区开展播种密度对不同成熟期大豆品种产量及水分利用效率的影响研究。结果表明,不同类型大豆品种随播种密度增加,其主要经济性状单株荚数、单株粒数、每荚粒数和单株粒重均呈下降趋势;播种密度对不同类型品种田间耗水量影响不显著;在播种密度9.0万株/hm²~27.0万株/hm²的范围内,晚熟品种‘汾豆78’、‘晋豆23’适宜种植密度为18.0万株/hm²,早熟品种‘中黄30’、‘晋豆19’适宜种植密度为22.5万株/hm²;适宜播种密度能够明显提高旱作区大豆籽粒产量和水分利用效率。     

鱼腥草主要病虫害调查方法与综合防治标准操作规程

摘 要：该文简述了鱼腥草主要病虫害种类,形态和症状及识别方法,提出了相应的调查取样方法和分级标准。根据鱼腥草主要病虫害的发生规律,参照《国家绿色食品A级农药施用标准》和《中药材生产质量管理规范(试行)》等条例的规定,归纳了一套鱼腥草病虫害防治标准操作规程,为鱼腥草GAP标准化生产提供了保障。     

回转机械转动惯量和动摩擦力矩测试方法

针对机械的回转系统,提出了一种整机测试春转动惯量和动摩擦力矩的新方法,分析了测试原理,推导了计算公式,并进行了初步的测试研究,实测证明,这种测试计算方法可以直接进行回转系统的惯量和摩擦力矩的测试,测试的对象为回转系统中的全部零部件,因此这种测试计算方法为整机回转系统的测试研究提供了依据。     

黑龙江省中熟大豆品种主要农艺性状演变趋势分析

黑龙江省近20年来中熟大豆品种遗传改进的明显趋势是株高、有效节数、一二粒荚数、三四粒荚数、单株荚数、单株粒数、每荚粒数和单株粒重增加,底荚高度略有增加。分枝数、蛋白质含量呈现先增后减的趋势．脂肪含量呈现先减后增的趋势,百粒重变化不大。株高、有效节数、三四粒荚数、单株荚数、单株粒数、百粒重与单株粒重呈显著或极显著的正相关。各性状对单株粒重的贡献大小依次为单株粒数、单株荚数、三四粒荚数、株高、有效节数、百粒重。提高单株粒数、单株荚数、三四粒荚数．兼顾株高、有效节数、百粒重是该区大豆育种的主攻方向。     

中间锦鸡儿fad2基因克隆与序列分析

油酸去饱和酶FAD2(fatty acid desaturase 2)在油酸中引第二个双键生成亚油酸,是负责植物体内产生多不饱和脂肪酸的第一步关键酶.本研究采用RT-PCR和RACE(rapid amplification of cDNA ends)技术,以中间锦鸡儿未成熟种子为材料,克隆了三个fad2基因(分别命名为fad2-2A、fad2-1A和fad2-1B).将这三个基因与汪阳东从中间锦鸡儿枝叶中克隆的fad2-2B(CaFAD2基因,GenBank登录号AY957394)一起进行了序列比对和分析.序列同源性比较结果表明,fad2-1A与fad2-1B同源性很高,fad2-1A编码的前283个氨基酸与fad2-1B的同源性高达98.9%,fad2-2B与fad2-1A的氨基酸序列同源性最低,只有64.7%.这四个基因的成功克隆,为进一步研究基因分工、调控方式打下了基础,为实现对锦鸡儿属植物脂肪酸成分直接、精确调控提供了保证,也是从一个物种中克隆四个fad2基因的第一次报道.     

Research Development of Higher Fungi in Plant Protection

高等真菌具有多种农用生物活性。概述了高等真菌在杀虫、抑菌、抗病毒、杀线虫活性等方面的研究进展,重点介绍高等真菌代谢产物作为杀菌剂的研究与应用,旨在为高等真菌在植物保护领域的进一步研究利用提供依据。     

BOT项目:紫之隧道开发建设的风险研判

对杭州紫之隧道项目建设风险进行了分析判断,总结出了BOT项目参与者如何对可能存在的项目建设风险进行鉴别、判断以及应对,提出了BOT项目风险控制的关键点,以使相关各方在实践中有所借鉴。     

北沙柳研究进展

北沙柳属杨柳科柳属植物,具有萌蘖力强,耐旱、耐盐碱和抗风沙的特点,是中国西北地区固沙造林的主要树种之一,兼具生态效益、经济效益和社会效益。我们综述了国内外近年来北沙柳的相关研究成果包括生长繁殖、生理生态特征、与真菌共生作用、防风固沙、分子生物学及产业化开发与利用,并对未来发展前景做出展望,提出优良种质资源收集、分子定向育种和资源合理开发等建议,旨在为北沙柳的种质资源库管理、资源保护和合理开发应用提供科学依据。