DNA methylation status in tissues of sheep clones

Genomic imprinting and DNA methylation play an important role in mammalian development. Many cloned animals showed heterogeneous DNA methylation profiles. However, there are fewer reports in cloned lambs because of a lack of genomic imprinting information. In this study, we investigated DNA methylation patterns in CpG islands and differentially methylated regions of putative imprinted gene Peg10 and imprinted genes Dlk1, Igf2R and H19 in cloned lambs. Five organs from two cloned lambs died shortly after birth and two normal controls were investigated. We observed normal DNA methylation profiles in cloned lambs. The imprinted genes Dlk1, Igf2R and H19 in livers, kidneys, hearts, muscles and lungs of the two cloned lambs exhibited relatively normal DNA methylation, except for Peg10 showing some differences between controls and cloned lambs. Our results indicate that somatic cell nuclear transfer-produced sheep exhibited relatively normal DNA methylation pattern and experienced normal DNA methylation reprogramming at imprinted loci.     

Research on the relationship between rainfall and reference crop evapotranspiration with multi-time scales

Limited by Fourier analysis and the selection of different wavelet base functions, the wavelet analysis method shows some deficiencies. For that reason, the empirical mode decomposition method is applied to analyze fluctuating periods and local features of annual rainfall and reference crop evapotranspiration (ET₀) from 1959 to 2002. With respect to the correlation between variables, the traditional time series analysis methods often assume that data sequences are stationary which result in “spurious regression.” Therefore, the cointegration theory is introduced to describe the long-term equilibrium relationship of rainfall and ET₀. At last, to understand the uncertainty between these two variables, the set pair analysis (SPA) method is used to present the identity, discrepancy, and contrary of rainfall and ET₀ with multi-time scales. The results reveal that rainfall and ET₀ have a complex relationship which may be related to El Nino, air–sea intersection, solar activity. Moreover, the fluctuation periods of rainfall and ET₀ also have similar short and middle period level, and slightly different long period level. The changes of rainfall and ET₀ present mainly the contrary and the discrepancy. This relationship will remain a long-term equilibrium.     

速生杨木改性研究进展

论述了近年来杨木改性优化的研究进展。对速生杨木材进行优化处理可使其在性能上接近或超过天然林木材,这对充分利用速生杨木资源、缓解木材供应紧张局面、促进林业经济良性发展等具有重要意义。目前对杨木改性研究主要集中在依靠物质填充处理和非填充处理两方面,主要包括浸注有机物、无机物的改性处理,压密化处理,高温热处理以及多种方法联合改性处理等。     

胆汁酸的生理功能及在畜牧业中的应用

<正>胆汁酸是胆汁的主要成分,在脂肪消化过程中起着重要作用。集约化养殖过程中频繁的应激无形中加大了动物肝脏的压力,肝脏分泌胆汁的功能受到抑制,     

北京市区县级农药市场存在问题与对策

保证农药市场秩序是确保农产品质量安全的一项重要措施。本文分析了区(县)级农药市场存在的主要问题,提出加强宣传与培训、建立健全相关制度、积极开展"农药标签"专项治理工作、搞好农药连锁配送等9项解决对策。     

平欧杂种榛高接换种技术

榛子营养丰富、用途广泛,市场经济价值较高,但由于平欧杂种榛发展时间短,可参照经验少,加之品种特性差异大,在山西省的种植过程中发现部分品种抗旱性差、生长势弱、丰产性差、果实质量低等不良表现,亟需高接改良。鉴于此,笔者结合多年的高接换种试验,在高接时间、高接方法、高接后管理等方面,总结了高接换种技术。实践证明该技术操作简便,速度快,成活率高达95%以上,且成形快、结果早。     

农业机械的事故原因分析与安全性设计原则

针对我国农机事故频发的情况,对农机事故进行分类研究。从机具设计与农机事故的关联性入手,结合工业设计理论对事故原因进行分析,指出农机事故发生本质上源于机具设计缺陷,研究各类农机事故的机具设计原因,提出预防农机事故发生的安全性设计原则。应用这些安全性设计原则,并在实践中不断扩充其具体细节,有助于从根本上解决农业机械的安全问题。     

蔬菜钾素营养的研究现状与展望

概述了目前国内外钾素在蔬菜作物栽培上的研究概况,从钾的植物生理学研究、钾对蔬菜作物产量、品质、抗逆性以及氮钾元素相互作用等方面作了比较详细的总结。钾几乎影响植物体内所有的代谢过程,包括光合作用、同化产物运转和分配、能量代谢、水分代谢、氮素代谢等许多方面。由于钾素影响了植株的代谢过程,从而对作物生长和产量形成具有积极作用,同时钾作为品质元素,可以提高果实的品质,但也有相反的报道。在总结前人研究的基础上对今后蔬菜钾素营养的研究提出了一些建议。随着蔬菜保护地的日益发展,科学地施用钾肥,明确钾及氮钾互作在蔬菜生理上的作用以及根据土壤和植株指标对土壤钾素盈亏进行诊断应是今后研究的主要方向。     

新型自走式割灌机结构分析

介绍了一种新型自走式割灌机.该机由行走、割灌、驾驶台及排障四个核心部分组成,可以锯切直径为10～80 mm的灌木.本文对其行走部分和驾驶台进行了系统介绍,并对其主要部件进行了参数设计.