均匀设计优化新疆主要葡萄品种ISSR-PCR反应体系

为了建立适用于新疆主要葡萄品种的ISSR-PCR反应体系,本研究以新疆不同葡萄品种DNA为模板,采用均匀设计法,对影响ISSR-PCR体系的Mg2+、dNTPs、引物、Taq DNA聚合酶等进行了U20(54)和U12(34)两轮优化,并在此基础上对退火温度及循环数进行了摸索.结果表明:在一定的浓度范围之内,Mg2+、...     

具连续变量差分方程的周期解与渐近周期解

研究了具连续变量的差分方程的周期解和渐近周期解，并分别获得了周期解和渐近周期解存在性的几个充分条件，我们的结果推广了Agarwal等人的相应结果．     

棉花不同种质材料棉铃各个时期发育研究

通过对棉花不同种质材料的棉铃在不同发育时期重量和长宽比的研究。结果表明,不同发育阶段的棉铃在重量和长宽比存在较大的差异;陆地棉和海岛棉的棉铃重量第5天和第10天变化趋势基本相同,但长宽比存在差异;两者的关系是长宽比越大,其铃重越轻;不同类型品种棉铃的长宽比是海岛棉〉圆锥形铃陆地棉〉卵圆形铃陆地棉〉圆形铃陆地棉,而重量恰好相反,为圆形铃陆地棉〉卵圆形铃陆地棉〉圆锥形铃陆地棉〉海岛棉;杂交棉相对于其亲本变化不明显。由此可见,棉花品种类型不同,棉铃形状的差异都是影响棉铃发育的重要因素。     

黄斑星天牛热空气处理技术研究初报

通过热空气、木材和锯末等 3种不同传热介质对黄斑星天牛 (Anoplophoranobilis)幼虫在不同温度条件下的热处理试验发现 ,热空气温度为 55℃ ,相对湿度为 30 %左右 ,经过80min即可全部杀死天牛幼虫 ;木材作为传热介质 ,在 55℃情况下经过 35min可完全杀死天牛幼虫 ;锯末作为传热介质 ,55℃ 2 5min、6 0℃ 2 5min、6 5℃ 1 0min和 70℃ 5min均可以杀死天牛幼虫 ;自然感染黄斑星天牛、芳香木蠹蛾东方亚种和杨干透翅蛾 ,规格为 1 0cm× 1 0cm× 32cm的木段 ,当加热使中心温度达到 55℃后 ,幼虫和蛹全部死亡。     

2013年安徽省中小规模种猪场猪伪狂犬病群流行率及风险因素横断面研究

[目的]了解安徽省中等规模种猪场猪伪狂犬病病毒（PRV）的感染情况。[方法]2013年8月至11月,开展了中等规模种猪场猪伪狂犬病群流行率和风险因素的横断面研究。通过简单随机抽样方法,选取了380个中等规模种猪场;用发现疫病的抽样策略,在每个种猪场采集了5份母猪血清,使用IDEXX公司猪伪狂犬病病毒gE-ELISA试剂盒进行了抗体检测。同时开展了问卷调查,并对种猪场之间PR传播潜在的29个风险因素进行了统计学分析。[结果]2013年安徽省中等规模种猪场猪伪狂犬病的群流行率为34.1%（95%置信区间29.3%~38.9%）;没有灭鼠措施（OR=2.5,95%置信区间：1.4~3.7,P<0.001）、购入种猪未经检测（OR=2.2,95%置信区间：1.1~4.2,P=0.024）、未免疫含PR 6种以上猪病（OR=2.0,95%置信区间：1.2~3.1, P=0.004）是2013年安徽省中小规模种猪场感染PRV的风险因素。Logistic 回归拟合度较好（P>0.05）,ROC曲线下面积为0.68（95%置信区间：0.62-0.73）。[结论]安徽省中等规模种猪场场间PR流行率较高,建议购入种猪前需进行检测,免疫含PR等6种以上猪病疫苗并定期灭鼠。     

糖萜素在水产养殖中的应用

本文对糖萜素饲料添加剂的特点、作用机理及在水产养殖中的应用、发展前景作一综述。     

变电站自动巡检管控系统研究与应用

通过带人工智能识别的高清摄像头、网盘录像机NVR、云端控制中心、信息存储数据库分别构成变电站自动巡检管控系统的采集层、传输层、站控层3层结构,并通过引入智能视频跟踪算法对摄像头采集的图像数据进行机器训练,从而保证对给定区域内目标跟踪的准确性。变电站自动巡检管控系统的应用,为实现电网作业实时、高效、全方位的巡检管控提供了技术平台,促进巡检管控作业向智能化、科技化发展。     

Duration of feeding linseed diet influences expression of inflammation-related genes and growth performance of growing-finishing barrows

The aim of the study was to investigate the effects of dietary linseed (rich in n-3 PUFA) on expression of inflammation-related genes and on growth performance of growing-finishing barrows. Two isoenergetic and isonitrogenous diets were formulated, one as the basal diet and the other containing 10% linseed. Twenty-four Landrace x Yorkshire barrows weighing 35 +/- 3.7 kg were randomly assigned to 1 of 4 treatment groups, with 6 pigs per group. During the entire experimental period of 90 d, these 4 groups of pigs were first fed the basal diet and then fed the linseed diet for 0, 30, 60, and 90 d before slaughter, respectively. Pig growth; messenger RNA (mRNA) expression of peroxisome proliferator-activated receptor-gamma (PPARgamma), IL-1beta0, IL-6, and tumor necrosis factor-alpha (TNF-alpha); and plasma concentrations of the 3 proinflammatory cytokines were measured and analyzed. Average daily feed intake did not differ among treatment groups (P > 0.05), but ADG (P < 0.05) and G:F (P < 0.01) responded quadratically to the duration of linseed diet feeding, and pigs in the 60-d treatment group had the greatest ADG and G:F. The mRNA expression of PPARgamma in loin muscle and spleen increased linearly (P < 0.01) with the duration of linseed diet feeding, whereas its expression in adipose tissue was not affected (P = 0.095). Tumor necrosis factor-alpha and IL-6 mRNA expression in muscle, adipose, and spleen, as well as serum concentration of TNF-alpha, decreased linearly (P < 0.01) with the duration of linseed diet feeding. Peroxisome proliferator-activated receptor-gamma mRNA abundance was negatively correlated with IL-1beta, IL-6, and TNF-alpha mRNA abundance both in muscle (R(2) = 0.63, P < 0.001) and in spleen (R(2) = 0.69, P < 0.001), and PPARgamma mRNA expression in spleen (R(2) = 0.59, P < 0.01) and muscle (R(2) = 0.52, P < 0.05) was negatively correlated with serum TNF-alpha concentration. There were also significant quadratic relations between ADG and expression of PPARgamma (P < 0.05) and splenic TNF-alpha (P < 0.05). These data suggest that intake of n-3 PUFA from the linseed diet led to significant decreases in the expression of proinflammatory cytokine genes, which may stimulate growth in growing-finishing barrows, at least in part, through a PPARgamma-dependent mechanism.