甘蓝型油菜耐低温生长生理生化响应机制

为提高甘蓝型油菜耐寒育种过程中的筛选效率，研究甘蓝型油菜耐低温机理，以8个不同抗寒性的甘蓝型油菜为材料，对低温条件下各材料的生物量、叶绿素、脯氨酸和相对电导率进行测定，发现在室外平均气温2.75℃时，抗性材料的生物量、叶绿素和脯氨酸的累积量都显著高于敏感材料，相对电导率没有显著差异；当平均气温7.52℃回升至12.39℃，抗性材料和敏感材料生物量的累积量无显著差异；在恒定低温10℃/4℃处理下抗性材料和敏感材料在处理前3周生物量均持续累积，但从第4周开始敏感材料新叶出现白斑，生物量减少，抗性材料老叶出现白斑，生物量维持不变。结果表明，耐低温材料在低温条件下叶绿素含量受到的影响较小，脯氨酸的积累量持续上升，具有较强的快速适应能力，在低温下具有显著的持续生长优势。     

饲料中添加不同水平的发酵豆渣对育肥猪生长性能、屠宰性能及经济效益指标的影响

为研究饲料中添加不同水平的发酵豆渣对育肥猪生长性能、屠宰性能及经济效益指标的影响,采用单因素随机设计试验,选择体重相近的健康育肥猪80头随机分成4组,每组20个重复,每个重复1头,1组饲喂基础日粮为对照组,试验2、3、4组分别在基础日粮中添加5%、10%、15%发酵豆渣,预试验7 d,试验期56 d。结果表明:(1)试验3、4组的平均日增重较1组比分别提高15.5%、14.1%(P<0.05),料重比较1组比分别降低14.7%、11.9%(P<0.05),试验2、3、4组的平均采食量均高于1组(P>0.05);(2)试验2、3、4组的宰前活重、屠宰率高于1组(P>0.05),试验3、4组的瘦肉率、眼肌面积较1组比分别提高12.1%、8.9%、12.3%、8.2%(P<0.05),背膘厚较1组比分别降低8.5%、7.0%(P<0.05);(3)试验3、4组毛利润较1组比分别提高22.4%、18.0%。综上,添加10%发酵豆渣可以提高育肥猪的生长性能、屠宰性能及经济效益。     

Inlfuence of FSH Treatment on Expression of CDC25A,TSSK3 and P53in Vitro Cultured Sertoli Cells of Calf

CDC25A, TSSK3 and P53 expressionsin vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of...     

不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响

通过构建氨单加氧酶基因(amoA)克隆文库,研究在红壤蔬菜田上只施用磷钾化肥(PK)、只施氮磷钾化肥(NPK)、施用腐熟有机肥(DNPK)和施用新鲜有机肥(FNPK)等4种不同施肥处理的土壤氨氧化细菌(AOB)和氨氧化古菌(AOA)群落多样性及与土壤脲酶活性的相关性。结果表明:施加有机肥处理(DNPK和FNPK)的蔬菜田土壤的AOB文库和AOA文库OTU数量和Shannon指数高于只施用无机肥(NPK和PK)处理的蔬菜田土壤;DNPK和FNPK处理的土壤优势AOB菌群为多形亚硝化叶菌(Nitrosolobus multiformis),比例分别为88.5%和68.5%,NPK和PK处理的土壤优势AOB菌群为亚硝化单胞菌属(Nitrosospirasp.),比例分别为54.8%和65.5%;DNPK、FNPK、NPK和PK处理土壤优势AOA菌群均为阿伯丁土壤亚硝化细杆菌侯选种(CandidatusNitrosotalea devanaterra),比例分别为90.9%、84.4%、77.8%和45.2%;施加有机肥处理(DNPK和FNPK)的土壤脲酶活性和氨氧化微生物的多样性指数都高于只施用无机肥处理(NPK和PK);AOA群落多样性指数与土壤脲酶活性呈显著正相关,而AOB群落多样性与土壤脲酶活性相关性不显著。总体来看,有机肥比无机肥处理提高了AOA和AOB群落多样性,且AOA在红壤蔬菜田土壤氨氧化过程中起着更为重要的作用。     

不同比例生物有机肥和复合肥混施对重庆地区烟叶农艺性状和品质的影响

为研究不同比例生物有机肥和复合肥混施对烟草农艺性状及品质的影响，采用田间小区试验，分别设置3种不同施肥处理，分析烟草不同生育期农艺性状、烟叶物理性状、烟叶外观等以及烟叶烟碱、总糖、还原糖等烟草品质指标。结果表明：单施复合肥处理(CK)前期烟草农艺性状优于不同比例生物有机肥和复合肥混施处理(T1和T2)，平顶期T1和T2的农艺性状则较CK组有明显优势；烟草中部叶片物理性状表现为T1处理最优，T2次之，CK最差；烟叶品质方面，T2处理烟叶外观质量有所改善，但其化学成分烟碱含量降低。烟叶品质总体表现为T2最优，     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

基于商业云平台植物工厂环境监测系统的研究与实现

植物工厂作为设施园艺发展的最高阶段,以其科技含量高、资源利用率高、高产优质等特点成为都市农业、垂直农业及太空农业等领域的研究热点。针对当前环境监测技术公网接入复杂、体积大及网络化管理程度低的问题,构建智能Wi Fi传感器,尝试采用商业云服务平台实现植物工厂内温度、湿度、光照强度及CO2浓度的采集并通过无线网络与云服务平台交互等功能,利用手机APP及PC端访问云平台两种方式完成植物工厂的远程监控。实验表明:本系统稳定性好、可靠性高,利用很少的网络带宽即可实时准确地将数据传输到云端。通过云平台的接入提高了数据服务的稳定性,无需重复构建云平台,提高了开发速度并降低应用成本,很好地满足了植物工厂环境监测的需求。     

多自由度剑麻收割机的设计与仿真制造

剑麻是一种广泛使用的硬质纤维的主要来源,被广泛的运用到各种行业,需求极大;但其收割现以手工为主,且因剑麻自身带有刺,加大了收割的难度,降低了效率。为解决剑麻的纯手工收割收割困难以及效率低的问题,提出一种多自由度剑麻收割机的机械,并申请了国家发明专利,得到授权。本文将运用UG、Sdams以及Mat Leb对该机械进行改进设计与仿真分析,并试制实物机械进行试验。试验结果表明:该机实现了剑麻收割的半自动化并提高剑麻收割的效率,可满足现行各行各业对剑麻的需求量。     

The innovation effect of low-carbon technology transfer from the perspective of carbon emission reduction demand: A case study of the Yangtze River Economic Belt in China

Reducing carbon emissions is the only way to solve the global climate problem, which obviously requires the joint efforts of all regions. From the perspective of carbon emission reduction demand, this research obtains the data of low-carbon patents and CO₂ emissions from 2000 to 2019, and then explores the innovation effect of low-carbon technology transfer in the Yangtze River Economic Belt (YREB). We draw the following conclusions: (1) Low-carbon technology transfer has a positive impact on low-carbon technology innovation. However, a large number of low-carbon technology transfers are concentrated between developed regions in the YREB at present. (2) No matter from the perspective of total amount or efficiency, carbon emission reduction demand can play positive moderating role in the process of exerting the innovation effect of low-carbon technology transfer, indicating that the transfer of low-carbon technologies to regions with high demand is efficient. (3) The moderating effect of carbon emission reduction demand in different regions has regional heterogeneity. Carbon emissions per unit of GDP plays a greater role in moderating the innovation effect of low-carbon technology transfer in the eastern region, while CO₂ emissions plays a greater role in the central and western regions. Therefore, we propose to strengthen the transfer of low-carbon technologies between developed and less-developed regions, and adopt differentiation strategies according to the actual situation of each region. Our research has deepened the understanding of environmental economic geography on the innovation effect of low-carbon technology transfer, and has reference significance for global low-carbon technology cooperation.