鸭传染性浆膜炎疫苗的研究

用从浙江省分离鉴定的鸭疫里氏杆菌RA-J、RA-L菌株研制疫苗,采用改进的液体培养和鸭胚培养工艺,抗原含量分别达120-186亿CFU／ml和280-310CFU／ml,免疫0.5ml/只,2MLD强毒攻击,平均保护率达94.2％（48／52）,免疫后第7天保护率达80％（16／20）,10天达93.8％（15／16）,疫苗在4℃-8℃条件下保存7个半月和1年的保护率达90％和85％。野外扩大试验和临床推广试用236万羽份表明,效果良好。     

适宜内蒙古通辽地区种植的优质苜蓿品种筛选试验研究

在内蒙古通辽地区研究了18个苜蓿品种的主要生产性能及越冬率.结果表明:加拿大苜蓿、美国杂花苜蓿、加拿大中间苜蓿、敖汉苜蓿、美国紫花苜蓿和草原3号在各方面都优于其他供试品种,其中敖汉苜蓿、美国紫花苜蓿和草原3号表现最优,适宜在该地区大面积推广种植.     

孵化场接种马立克氏病疫苗的新方法

随着禽病发生的日益复杂化,孵化场接种马立克氏病疫苗面临很多挑战,大多数从业者选用1日龄接种抗生素来预防雏鸡前期细菌性疾病,于是接种马立克氏病疫苗和接种抗生素使用同一把注射器,加之高温蒸煮的损耗,导致手动注射器在接种剂量准确度等诸多方面对家禽生产者造成很大的风险,所以由于器具的使用问题而导致在田间发生马立克氏疾病的可能性增加,严重困扰着行业内的从业者.     

母猪饲粮添加甜菜碱对巴马香猪哺乳仔猪血液指标的影响

本试验旨在研究母猪饲粮添加甜菜碱对巴马香猪哺乳仔猪血液指标的影响。选取3~7胎次妊娠巴马香猪40头,随机分为2组,每组20头,单栏饲养。对照组饲喂基础饲粮,试验组在基础饲粮中添加3.5 kg/t甜菜碱。试验期为母猪配种后第3天至分娩后第21天。试验结束时,从每组随机选取10窝仔猪,每窝选取1头仔猪采血,测定血浆激素含量、抗氧化指标和游离氨基酸含量。结果表明:与对照组相比,甜菜碱组哺乳仔猪血浆胆囊收缩素(CCK)、酪酪肽(PYY)、瘦素(LEP)、丙二醛(MDA)、过氧化氢(H2 O2)和丙氨酸(Ala)含量显著降低(P<0.05),血浆鹅肌肽(Ans)、1-甲基组氨酸(1⁃MeHis)和3-甲基组氨酸(3⁃MeHis)含量显著升高(P<0.05),血浆苏氨酸(Thr)(P=0.069)、谷氨酸(Glu)(P=0.055)和肌肽(Car)(P=0.055)含量呈下降趋势,血浆亮氨酸(Leu)含量呈升高趋势(P=0.069)。综上所述,母猪饲粮添加甜菜碱可降低哺乳仔猪血浆摄食相关因子水平,减弱机体的氧化水平,改变血浆游离氨基酸含量,有利于仔猪的生长发育与机体健康。     

大蒜精油对灰茶尺蠖的室内毒力及田间防效

采用大蒜精油对灰荼尺蠖进行了室内及田间药效试验,结果表明大蒜精油对灰茶尺蠖的致死中浓度(LC50)为1038.60 mg/L,50％大蒜精油100～250倍液对灰荼尺蠖低龄幼虫具有良好的速效性和持效性,建议生产中可在灰茶尺蠖1～2龄幼虫发生期喷施100～250倍液大蒜精油.这一结果为开发大蒜精油作为茶树主要害虫的新型低...     

Effect of eleutheroside B/E on proliferation and expression of PPARγ and TGF-β1 in HBZY-1 cells cultured with high glucose

AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression.     

不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响

通过构建氨单加氧酶基因(amoA)克隆文库,研究在红壤蔬菜田上只施用磷钾化肥(PK)、只施氮磷钾化肥(NPK)、施用腐熟有机肥(DNPK)和施用新鲜有机肥(FNPK)等4种不同施肥处理的土壤氨氧化细菌(AOB)和氨氧化古菌(AOA)群落多样性及与土壤脲酶活性的相关性。结果表明:施加有机肥处理(DNPK和FNPK)的蔬菜田土壤的AOB文库和AOA文库OTU数量和Shannon指数高于只施用无机肥(NPK和PK)处理的蔬菜田土壤;DNPK和FNPK处理的土壤优势AOB菌群为多形亚硝化叶菌(Nitrosolobus multiformis),比例分别为88.5%和68.5%,NPK和PK处理的土壤优势AOB菌群为亚硝化单胞菌属(Nitrosospirasp.),比例分别为54.8%和65.5%;DNPK、FNPK、NPK和PK处理土壤优势AOA菌群均为阿伯丁土壤亚硝化细杆菌侯选种(CandidatusNitrosotalea devanaterra),比例分别为90.9%、84.4%、77.8%和45.2%;施加有机肥处理(DNPK和FNPK)的土壤脲酶活性和氨氧化微生物的多样性指数都高于只施用无机肥处理(NPK和PK);AOA群落多样性指数与土壤脲酶活性呈显著正相关,而AOB群落多样性与土壤脲酶活性相关性不显著。总体来看,有机肥比无机肥处理提高了AOA和AOB群落多样性,且AOA在红壤蔬菜田土壤氨氧化过程中起着更为重要的作用。     

稻粉虱的发生及其化学防治药剂筛选

稻粉虱Aleurocybotus indicus David et Subramaniam, 属半翅目粉虱科, 以刺吸式口器为害水稻叶片而导致产量损失, 是我国闽?赣?浙等稻区的一种偶发性水稻害虫, 近20多年已鲜有报道, 但2019年-2021年在中国水稻研究所富阳试验农场稻田暴发?本文描述了稻粉虱的为害症状和形态特征, 并配以原色照片; 克隆得到了稻粉虱线粒体DNA COⅠ基因片段, 发现其与烟粉虱Bemisia tabaci序列一致性最高(85.80%); 田间调查表明, 富阳试验农场及周边稻田稻粉虱均有不同程度的发生, 但前者发生量明显较高(每个分蘖最高可达251头), 推测可能与农场温室内的稻粉虱越冬虫源有关; 利用稻苗浸渍法评估了不同作用机制的7种杀虫剂对稻粉虱的毒力, 发现双丙环虫酯?氯虫苯甲酰胺?氟啶虫胺腈?氟啶虫酰胺和溴氰虫酰胺对稻粉虱成虫均有较好的杀虫活性, 其中前4种药剂推荐中剂量处理后72 h的校正死亡率均可达100%, 其LC50分别为3.028?3.769?6.405 mg/L和7.894 mg/L?本研究可为我国稻粉虱的识别诊断和化学防治提供重要依据?     

The Method of Diagnosing Brain-dysfunction Based on Membership Function and BP Neural Network

Membership functions.formed with the characteristic values obtained by applying the continuous reaction time (CRT) theory from controls and heptic encephalopthy and proposedin this paper.whith these membership functions,the CRT data to be diagnosed are calculated in advance,and then discriminated by BP meural network to differentiate patients with or without braindysfunction.The troubles of low accuracy and efficiency,encountered in training BP network withfuzzy samples.scattered data and extended samples,are solved.     

小麦随机多交后代中抗条锈(Puccinia striiformis)性的选择

本试验用16个被认为可能具有水平抗性的小麦品种为基础材料,采用乙烯利化学杀雄或太谷核不育为手段进行随机多交,以相对抗病性综合指数(RRCI)表示抗病性强度,研究了小麦条锈病水平抗性的遗传力及人工选择下的遗传进度,结果指出:1)随机多交的后代系统中,出现了一些抗条锈性显著高于优良亲本的系统。2)抗条锈性的遗传力较高:化