利用手工克隆技术培育体细胞克隆莱芜猪

为探讨手工克隆技术在克隆中国地方猪种的可行性,本研究组利用手工克隆技术平台,以中国地方猪品种莱芜猪的耳皮肤细胞作为核移植的供体细胞,以体外成熟的猪卵母细胞为受体细胞,进行体细胞克隆猪培养,成功培育了25头克隆莱芜猪。表明手工克隆技术可应用于莱芜猪体细胞克隆,为莱芜猪的保种及育种提供了新的途径。     

我市畜产品的安全问题和对策

随着国民经济的不断发展,人民生活水平的逐步提高,肉、蛋、奶等畜禽产品在食品结构中比重越来越大,人民群众对健康日益重视,对畜产品安全卫生要求也越来越严格。同时,加入WTO后,我国产品的低成本经营在国际市场上具有价格优势,但由于没有建立完善的畜产品质量保障体系,在国际市场上有优势无市场。因此,畜产品的质量日益受到人民群众和政府的重视,迅速提高畜产品质量,抓好安全食品生产,已成当务之急。1我市畜产品安全存在的主要问题1.1疫病仍然严重。由于政府和广大畜牧兽医人员的不懈努力,疫病对畜禽的危害明显减轻,因疫病造成的损失明显…     

“益母草散”注射液的研究

随着集约化养殖的不断发展。畜禽所患疾病的种类在增加,药物的耐药性和副作用不断产生,从而导致许多药物的疗效降低。中药是几千年来中国人民与疾病斗争的结晶,而且无毒副作用、无残留、不产生耐药性,对疾病的预防和治疗均有较好的效果。中药传统剂型对动物使用不方便,而中药单方对防治疾病有一定的局限性。所以,本次试验选择益母草散做成复方针剂,日的是探索中药针剂的制备方法。试验结果证明：“益母草散”的针剂制备对以后复方中药针剂的开发和研究提供了依据。     

我国部分地区病死畜禽无害化处理中心病原灭活情况调查

为了解我国病死畜禽无害化处理环节病原灭活效果,2020年对11个省(自治区)52个病死畜禽无害化处理中心开展抽样检测、问卷调查.通过对样品进行病毒核酸检测和病毒核酸阳性样品病原活性检测发现,化制法、发酵法、碳化法、酸解法均可灭活非洲猪瘟、猪瘟、口蹄疫和猪繁殖与呼吸综合征等4种疫病病原.通过问卷调查发现,无害化处理中心因...     

评教三结合 推进名师工程的探索与实践

在我国，高等教育由“精英教育”进入国际公认的“大众化教育”时期的背景下，如何保证教学质量，是摆在高等教育管理工作者面前的重要课题。我们结合学校实际开展“评教三结合”，在加强教学管理，推进名师工程方面进行了探索与实践。     

鸭瘟病毒的PCR检测及其产物分析

根据基因库中已有的鸭瘟 Ul6的序列 ,合成一对引物 ,对 3株鸭瘟病毒进行 PCR扩增 ,均扩增出大小约 42 0 bp的特异性片段。进行测序 ,结果表明 3个毒株扩增后的片段均为 42 1bp。经重复实验后确定最佳反应条件 ,按该条件对两例临床病料进行检测 ,结果均出现大小约为 42 0 bp的特异片段。PCR产物与 L ake Andes株的相关序列比较 ,广东毒株与 L ake Andes株同源性较高     

四川农村养猪日粮及平衡饲养技术初探

在四川养猪主产地的不同地理生态区选点,深入农户广泛开展农村养猪常用饲料及日粮调研,采集饲料样品92个,分析测定铁、铜、猛、锌、钴、碘和硒的含量,将四川农村养猪日粮总结划分为:(1)玉米、青料日粮;(2)甘薯、青料日粮;(3)玉米、小麦、青料日粮;(4)玉米、大麦、青料日粮。并对各日粮的营养含量作了概算和评定。在此基础上,提出适合四川农村养猪日粮的添加剂预混料和浓缩料配制建议以及浓缩料主要营养指标推荐值。依此,开展了4种日粮的6批44组347头肉猪的饲养试验。从中优选出一批饲料配方,已在11个地市的40余家厂试产,省内外广大农村养猪中应用,有效地促进了饲料工业和农村养猪生产的发展。研制的甘薯青料日粮及平衡饲养技术,已列专题在省内大面积推广应用。经济效益和社会效益十分显著。     

Canine Haematopoietic Chimerism Analyses by Semiquantitative Fluorescence Detection of Variable Number of Tandem Repeat Polymorphism

Canine models are successfully applied to the study of haematopoietic stem cell transplantation (HSCT). Monitoring of haematopoietic donor/recipient chimerism is of major significance in detecting and quantifying engraftment or graft rejection of the donor-derived haematopoietic cells after transplantation. Radioactive analyses of polymorphic microsatellite markers are commonly used for chimerism analyses. We describe an improved, non-isotopic method that is based on the analysis of microsatellite markers in donor and recipient cells using capillary electrophoresis and fluorescence detection. Artificial mixtures of donor and recipient DNA that were generated from peripheral blood mononuclear cells from dog leukocyte antigen-identical siblings were used to analyse the sensitivity of the assay. DNA from dogs that had received HSCT were also analysed in order to demonstrate the feasibility of the method in vivo. For chimerism analyses, six different microsatellite loci were systematically amplified using fluorescent PCR primer. The fluorescent polymerase chain reaction products were separated by capillary electrophoresis using POP4 on a 310 ABI Prism Genetic Analyzer. After electrophoresis, fluorescence signals were automatically sized and quantified using GeneScan software. The method described provides an accurate assessment of haematopoietic chimerism in the canine model with significantly reduced hands-on time compared to conventional gel electrophoresis.     

A short note on the horizontal and vertical movements of a whale shark, Rhincodon typus, tracked by satellite telemetry in the South China Sea

Whale sharks, a global migratory species, are often reported entangled in fishing nets in coastal areas of China. The effectiveness of conservation measures has been constrained by very limited knowledge on their movements and preferred habitats in the coastal areas of China. For the first time, we tracked the movements of 2 whale sharks by satellite telemetry in Mainland China. The tracking results of 1 whale shark revealed that it travelled in the South China Sea in a south-eastern direction, parallel to the eastern coast of Vietnam. Total distance travelled was 1018 km, in approximately 74 days, with a mean speed of 14 km per day. It appeared to head towards the cool upwelling zones in southern Vietnam at the time of the tag's detachment. In our study, it was observed that this whale shark was a surface dweller and spent approximately 45% of its time above 10 m water depth and 90% of its time above 50 m depth. It also tended to stay in water temperatures between 27 and 30 °C, and was rarely recorded in water below 20 °C. This preliminary study indicates the importance of shallow waters as the foraging habitat for whale sharks, and has implications for their management and conservation.