High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.     

不同稀释液对藏獒精液保存效果的影响

比较了8种稀释液在藏獒精液冷冻保存和低、常温保存(5 ℃,10 ℃,15 ℃,20 ℃)的效果.结果表明:2号和8号稀释液在冷冻保存时效果最好,精子活力和顶体完整率分别达到0.40和49%,与对照组相比差异显著(P<0.05).在藏獒的低温、常温保存试验中,所有稀释液在15 ℃时保存时间最长,综合考虑,7号液保存效果最好;在15℃时精子平均存活时间为103 h,显著高于对照组和其它稀释液(P<0.05).     

薛家湾镇道路绿化模式研究

在对薛家湾镇现有的11条主、次干道路的总体绿化状况实地调查的基础上,对薛家湾镇的开源路、蓝天路、塔哈拉路道路绿化模式进行研究,并提出设计方案,为提高薛家湾镇生态环境质量、改善城市面貌提供参考依据。     

贵阳地区草地害虫种类调

随着国家对畜牧业的重视并加大对畜牧业的投入,牧草作为养殖所需要的基本原料,是畜牧业发展的基础,因而也得到了高度的重视,并在国内外部分地区得到了大面积的种植和推广.     

学校档案管理工作进展的思考

目前学校档案管理工作中存在的一些问题亟待解决，档案管理工作的基本原则包括整体性原则、服务性原则和社会化原则，学校的档案管理工作唯有创新才有出路，重点应加强观念创新，技术创新和知识创新。     

红米湘晚籼12号的综合利用途径

概述了红米湘晚籼12号的产量、米质、抗逆性等品种特性,检测分析了该品种与普通稻(金优207)的米糠和精白米营养成份,根据该红米品种特性及营养特征、生产与加工情况、销售与市场前景,提出几种利用红米湘晚籼12号的创新开发途径.     

连锁超市在我国农村地区发展的问题及对策研究

在分析归纳连锁超市在我国农村地区发展动因的基础上,指出了发展中面临的问题,并根据我国农村消费品市场的自身特点,提出了解决问题的对策。     

Effects of Three Drugs on Membrane Metabolism Against Cysticerci cellulosae in vitro

To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one.     

运用模糊数学和灰色系统理论分析评价夏播花生区试品种

运用模糊数学和灰色系统理论，对2007年河南省夏播花生区试品种进行了多因素综合评价和分析，结果表明：周花4号评价指标是0．7089，综合表现最优；其次是W9847—1—3—4—1，其综合评价指标是0．6352；豫花6号评价指标是0．3630，综合表现最差。此结果与试验地实际表现基本相一致。     

阅读疗法在糖尿病患者健康教育中的应用

昆明医学院第一附属医院图书馆联合医院糖尿病科，选择30名住院患者作为健康教育 对象，向其发放推荐书目和阅读资料。比较阅读治疗前后SCL-90结果得出的结论是，将阅 读治疗与健康教育结合起来，可以增加患者治疗的依从性，起到了良好的健康教育效果。