三峡水库消落区植物群落结构及其季节性变化规律

为揭示三峡水库消落区出露期间植物群落结构特征的季节性变化规律,于2017年4、6和98月,设置了15个调查样地,并根据高程将消落区分为145~155 m、156~165 m和166~175 m等3个区域,并设置未水淹区域（高程176~-185 m）为对照。结果表明,消落区出露时间显著影响着植物群落的组成,随着出露时间的延长,消落区植物群落优势种及其优势度变化规律因植物的生活型不同呈现出相反的变化规律。从4月至8月,优势植物狗牙根、牛鞭草、喜旱莲子草等多年生草本植物优势度下降,鬼针草、苍耳、醴肠、水蓼、野胡萝卜、狗尾草等一年生草本植物优势度增加。而未水淹区植物优势种及其优势度变化规律不会因生活型不同而表现出不同的规律,不同地点的植物群落优势种差异较大,相对来说艾蒿较为优势,其次为小飞蓬。高程也是影响植物群落特征的主要因子,消落区植物群落Shannon-Wiener指数、Margalef指数、Simpson指数、植物高度均值显著低于未水淹区域,随着高程的增加,Shannon-Wiener指数、Margalef指数、Simpson指数生物多样性指数及、植物高度均值呈增加趋势。三峡水库消落区之所以呈现出目前的植物群落分布特征,植物内在的适应机制包括植物本身的冬季耐水淹能力、夏季抗旱能力、抗病虫害能力以及植物的及其繁殖对策、种源扩散对策等是主因,而外界环境条件,包括消落区土层厚度、地形坡度、土壤基质氮磷等营养盐,以及受水淹持续时间、水淹深度、高程、消落区出露时间等是其主要驱动因子。     

三峡库区26条支流浮游甲壳动物的群落结构

2010年4月至2011年6月分4次对三峡库区26条支流及长江干流浮游甲壳动物群落结构进行调查,采集浮游甲壳类的定量和定性标本,共计152个采样点.结果表明,4次采样共鉴定出浮游甲壳动物50种,其中枝角类34种,桡足类16种.浮游甲壳类以常见的广温性种类为主,均为长江流域常见种.三峡库区浮游甲壳类动物具有明显的季节性变化特征和纵向分布特征,春季的现存量和种类明显高于冬季,相同支流上游的现存量和种类一般低于回水区.     

Overexpression of SSTR2 inhibits growth of SSTR2-positive tumors via multiple signaling pathways

AIM: To study the anti-proliferation effect of overexpressed SSTR2 in experimental cancer with different profiles of endogenous SSTRs expressions and the possible signaling pathways involved. METHODS: In the first experiment, the growth of the tumor xenografts of the inoculated capan-2 cells and A549 cells overexpressing SSTR2 or LacZ was investigated in nude mice. In the second experiment, the adenoviral vector expressing SSTR2 were introduced into experimental capan-2 xenografts by intratumoral injection. The growth inhibition of these experiment tumors was observed and the potential influences on different signaling pathways were analyzed by immunoassays. RESULTS: Overexpression of SSTR2 inhibited the growth of tumors with different profiles of endogenous SSTRs, including experimental capan-2 xenografts that had endogenous SSTR2 expression. Overexpression significantly affected a number of components in apoptotic pathway, MAPK pathway and angiogenesis. CONCLUSION: SSTR2 is a promising candidate for gene therapy in a wide spectrum of cancers.     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.     

番茄晚疫病抗病育种及分子生物学研究进展

对国内外近年来在番茄晚疫病菌的病原学、抗病资源的筛选鉴定、抗病机制的研究及分子生物学方面取得的进展进行了综述,并展望了今后的研究重点和方向。     

不同基质栽培对番茄叶片光合作用和根系ATP酶的影响

以腐熟玉米秸、麦秸、菇渣、锯末等农产废弃物添加土壤后配成有机基质进行番茄栽培试验,探讨不同基质栽培对番茄叶片光合特性和根系ATP酶活性的影响。结果表明,有机基质栽培较土壤栽培植株同化产物积累增加,番茄光合速率、细胞间隙CO₂浓度、呼吸速率均显著提高。有机基质栽培Fv/Fm、ΦPSⅡ、qP显著升高,P-H⁺+ATPase、P-Ca²⁺-ATPase、V-H⁺-ATPase、V-Ca²⁺-ATPase活性提高。有机基质栽培番茄通过提高根系质膜和液泡膜ATPase活性、增加番茄的光合速率、细胞间隙CO₂浓度,提高Fv/Fm、ΦPSⅡ、qP,提高光化学效率,增强营养吸收,从而促进番茄生长和同化物形成。     

西瓜EST-SSR分子标记的筛选

利用国际共享获得的820条EST序列,自主设计出79对EST-SSR引物,其中有32对引物经过PCR扩增出稳定清晰的电泳条带,可以作为西瓜和相关物种的新的EST-SSR分子标记。     

竹酢液对杉木苗木生长的影响

以1年生Ⅰ级杉木裸根苗为研究对象,通过盆栽试验,比较喷施及土壤施入两种施用方法及7个不同浓度处理下,竹酢液对苗高、地径以及枝叶生物量、根系生物量和主干生物量影响,同时对不同处理下苗木净光合速率的变化特征进行了比较分析。结果表明：竹酢液合理使用可以明显促进苗高和地径的生长、提高枝叶生物量和根系生物量;竹酢液可以提高苗木的净光合速率,改善光合作用;P300和P800两种浓度和施入方法促进杉木苗木生长的作用最明显。     

贵州柳杉云毛虫发生特点与防治

为了有效控制柳杉云毛虫Hoenimnema roesleri Lajonquière在贵州的发生、危害及蔓延,对柳杉云毛虫的生物学习性进行了初步调查,对其形态进行了描述,对其防治效果进行了后续观察,总结提出了化学、人工、营林相配套的综合防治技术措施。     

高产优质长粒型粳稻新品种垦育25的选育及栽培技术

垦育25是河北省农林科学院滨海农业研究所育成的长粒型粳稻新品种,具有丰产性好、米质优、抗病能力强等优点。河北省区域试验平均产量694.5 kg/667 m²,较对照增产11.4%,米质达到国家优质3级米标准,田间表现抗倒性和抗病性强,适宜机械化栽培。2020年通过河北省农作物品种审定委员会审定,并被秦皇岛市列为主推水稻品种。