全文获取类型
收费全文 | 30949篇 |
免费 | 1846篇 |
国内免费 | 3098篇 |
专业分类
林业 | 2026篇 |
农学 | 1699篇 |
基础科学 | 1568篇 |
3065篇 | |
综合类 | 15305篇 |
农作物 | 2365篇 |
水产渔业 | 1383篇 |
畜牧兽医 | 4915篇 |
园艺 | 2321篇 |
植物保护 | 1246篇 |
出版年
2024年 | 249篇 |
2023年 | 610篇 |
2022年 | 1461篇 |
2021年 | 1376篇 |
2020年 | 1338篇 |
2019年 | 1230篇 |
2018年 | 915篇 |
2017年 | 1440篇 |
2016年 | 985篇 |
2015年 | 1528篇 |
2014年 | 1598篇 |
2013年 | 1894篇 |
2012年 | 2671篇 |
2011年 | 2732篇 |
2010年 | 2530篇 |
2009年 | 2259篇 |
2008年 | 2353篇 |
2007年 | 2118篇 |
2006年 | 1760篇 |
2005年 | 1351篇 |
2004年 | 917篇 |
2003年 | 575篇 |
2002年 | 574篇 |
2001年 | 536篇 |
2000年 | 468篇 |
1999年 | 184篇 |
1998年 | 22篇 |
1997年 | 30篇 |
1996年 | 22篇 |
1995年 | 20篇 |
1994年 | 17篇 |
1993年 | 20篇 |
1992年 | 26篇 |
1991年 | 13篇 |
1990年 | 7篇 |
1989年 | 1篇 |
1987年 | 13篇 |
1986年 | 11篇 |
1985年 | 1篇 |
1981年 | 4篇 |
1979年 | 1篇 |
1966年 | 2篇 |
1962年 | 8篇 |
1958年 | 1篇 |
1956年 | 19篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
241.
对重庆市涪陵区城镇化化发展和金融发展现状进行综合分析,并对当下金融支持城镇化发展中出现的困难提出了相应的对策建议。 相似文献
242.
新疆农业高效节水工程资产运行管理模式比较分析 总被引:1,自引:0,他引:1
新疆农业高效节水工程项目资产运行管理模式是在新疆节水灌溉工程大规模建设过程中逐渐形成的,在对新疆农业高效节水工程项目资产管理模式归纳总结的基础上,对各种模式优缺点采用定性分析的方法进行了比较,分析了存在的问题,对进一步提高节水工程项目资产管理水平提出了一些建议。 相似文献
243.
244.
通过灌区试验研究,探讨了披碱草(Elymus dahuricus Turcz)不同土壤水分条件下蒸腾速率、净光合速率、气孔导度、胞间CO2浓度、叶面温度、水蒸汽浓度差的日变化规律,分析了蒸腾速率与气体交换特征参数的关系。试验结果表明:光合速率、蒸腾速率随着土壤含水量的降低而下降;蒸腾速率、净光合速率、气孔导度、水蒸汽浓度差在自然状态和充分供水状态下均呈双峰曲线特征,蒸腾速率、净光合速率、在凋萎缺水状态下出现三峰特征;胞间CO2浓度的日变化规律与蒸腾速率、净光合速率的日变化规律相反,呈W型;蒸腾速率与水蒸汽浓度差的相关度极高,线性关系明显。 相似文献
245.
246.
LI Qin CHEN Yu-cun ZHAO Feng-yong ZHANG Jia-min ZHANG Shu-hui ZHU Zi-yan 《中国畜牧兽医》2016,43(7):1850-1855
In this study,we aimed to determine whether bear had different blood group systems.Whole blood samples were collected from 40 Asiatic Black bears in Fujian province,China.Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol.Plasma was further analyzed by mass spectrometry.The plasma from four bears had antibodies,possibly IgM isoform,which could agglutinate RBCs from 30 bears.Blood samples from 10 bears were tested by human blood typing reagents.The results showed that 4 bears had blood type like human type O,while 6 bears had like human type B.Plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda (Ailuropodamelanoleuca).We suggested that Asiatic Black bear might have at least one blood group system with two blood types.If the sick bear needs blood transfusion,a cross-matching test was necessary.Moreover,giant panda might receive blood from Asiatic Black bear in case of emergency. 相似文献
247.
WANG Chun-xin ZHAO Yun-hui ZHAO Zhuo ZHU Jing-liang ZHAI Bo ZHAO Feng-jiang ZHANG Ming-xin 《中国畜牧兽医》2016,43(1):108-113
The experiment aimed to find out the effects of dietary crude protein levels on growth performance, digestion and metabolism and serum biochemical indexes of Super Merino lamb after weaning.According to the dietary crude protein level, 64 Super Merino weaned lambs were divided into four groups, which the crude protein levels were 13.25%(group Ⅰ), 14.33%(group Ⅱ), 15.53%(group Ⅲ) and 16.60%(group Ⅳ), respectively.The experiment lasted for 60 days, 30 days for the early stage and 30 days for the later stage;And on the 30th and 60th day, blood samples were collected from one head sheep chosen from each replication, and at the end of the feeding test, 3 lambs were randomly selected from each experiment group for a 15 days digestion and metabolism experiment.The results showed that ADFI and ADG in the early and total trial period were significant differences(P<0.05), ADFI and ADG of group Ⅲ were higher than others.The apparent digestibility of nutrients and ME/DE were not significantly changed(P>0.05).In the detection of serum biochemical indexes, there was no significant difference in the earlier stage(P>0.05);At the end of the test, PK and CK showed significant difference between groups(P<0.05).PK presented a rising trend as the protein level improved, CK of group Ⅰ was higher than other groups.In Super Merino lamb early weaning diet, 15.53% protein level in the diet could significantly improve ADG, the apparent digestibility of nutrients, metabolic energy digestibility.At the end of the test, PK and CK were affected by dietary crude protein level significantly. 相似文献
248.
GUO Qin-qin FAN Zong-xing Hao Hai-sheng LIU Yan ZHAO Xue-ming ZHU Hua-bin DU Wei-hua 《中国畜牧兽医》2016,43(2):477-486
Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research. 相似文献
249.
RAN Xu-hua ZHANG Yao CAO Si LU Yuan-he ZHANG Ling-ling LIU Yang-yang NI Hong-bo ZHU Zhan-bo WEN Xiao-bo 《中国畜牧兽医》2016,43(5):1368-1373
To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg2+ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China. 相似文献
250.
为研究肠炎沙门氏菌鞭毛素对其生物膜形成的影响,本研究利用构建的肠炎沙门氏菌鞭毛素编码基因fliC缺失株,检测其缺失性修饰后的生长表型变化及其对生物膜形成的影响。结果显示在表型检测试验中,fliC缺失株在电镜下观察呈无鞭毛形态,在半固体培养基上缺乏运动性,并且不能与肠炎沙门氏菌鞭毛单克隆抗体发生可见的凝集反应。生物膜形成能力定性试验结果表明,肠炎沙门氏菌fliC缺失株生物膜形成大量减少,并且生物膜脆弱,而其回补株能够较好地恢复生物膜的形成;生物膜定量结果进一步证明,鞭毛素fliC基因缺失后肠炎沙门氏菌形成生物膜的能力下降50%左右,以上结果显示鞭毛在肠炎沙门氏菌体外生物膜形成中具有重要作用。 相似文献