Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.     

柔嫩艾美耳球虫感染鸡盲肠和脾脏一氧化氮合酶的表达

采用NADPH—d(nicotinamide adenine dinucleotide phosphate—diaphroase)组织化学法观察了雏鸡感染柔嫩艾美耳珠虫(E．tenella)后一氧化氮合酶(N0S)在盲肠和脾脏中的分布与表达情况。试验结果表明，所有正常鸡盲肠粘膜下层和肌层均有较深的着色，根据以往的资料和N0S的表达特性初步判断为神经元型N0S(nNOS)；试验鸡在感染后3～5d，盲肠粘膜上皮和肠腺上皮也有较深的着色，并从感染后7d开始着色减弱；而对照组鸡盲肠粘膜上皮和肠腺上皮以及对照组和试验组的脾脏几乎不着色或着色很浅。试验结果提示，盲肠粘膜上皮和肠腺上皮的着色可能是诱导型N0S(iN0S)表达的结果，而由其产生的N0参与雏鸡球虫感染过程。     

The use of a Giardia vaccine as an immunotherapeutic agent in dogs.

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.     

红锥人工幼林施肥试验研究

试验研究表明，合理施肥能促进红锥人工幼林的生长，施肥量水平不同，其促生效果也不同，N、P、K肥对红锥人工幼林的生长具有同等重要的特性。综合本试验结果，红锥人工幼林的初步合理施肥量为每株尿素100g、钙镁磷150g、氯化钾50g，养分配比为2：3：1(尿素：钙镁磷：氯化钾)，超过该施肥量，已没有增产效益。特别应指出的是，在本试验的立地条件下，当每株施肥量尿素超过300g或钙镁磷肥超过450g或氯化钾超过150g时，均有可能发生肥害，需引起重视。     

矸酆恐?,垩白率与糙米率、蛋白

Χ灾绷吹矸酆康挠跋焓钦?,直接影响比间接影响大。Study on the correlation between rice quality and agronomic traits of quality riceNIE Cheng rong, WEN Yu hui, WANG Yun bo, CHEN Ming zhong,LI Mei, LIU Dao quan(Agronomy Department, Foshan University , Nanhai 528231,China)The correlative analy     

Multi-sectoral Participatory Urban Forestry Development in Philippines Metropolitan Manila

The Luntiang Kamaynilaan Project (Metro ManilaGreening Project) launched in 1988 planted trees inmajor thoroughfares, center islands, school premisesand other idle public lands, was basically a governmenteffort. When the funds ran out, the project also fizzledout. In both projects however, it is evident that the keyfactor is peoples participation.Gaining lessons from the past two projects, thegovernment established a collaborative effort ofgovernment and non-government organizations. TheCl…     

提高阅卷与试题分析速度的有效方法探讨

分析了核对、打孔、光照透明、光电机器阅读和扫描等几种快速手工和自动阅卷评分方法。采用我校目前广泛使用的“成绩记载”中的浮动“阅卷面板”鼠标点击，以及自动阅读机与“成绩记载”间的“数据转换”程序，可大大提高成绩录入、成绩分析和试题分析速度。     

库存本阅览室与外借库的合并管理

我馆在2005年将中文图书库存本阅览室与中文图书外借库合并成中文书库,进行统一管理。本文结合工作实际,分析了书库合并前分开管理的弊端,阐述了二者合并的意义,提出了合并后的具体管理措施。     

Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.     

田间小棚繁殖烟蚜茧蜂的繁蜂效果研究

研究了田间小棚繁殖烟蚜茧蜂的繁蜂效果,比较了在不同接蜂量和蚜量范围条件下的僵蚜数量及其羽化情况。接蜂量和接蜂时的蚜量是影响僵蚜数量的2个重要因素,以接蜂时的蚜量对繁蜂效果的影响更为明显。在接蜂量为蜂(♀)蚜比1∶100、蚜量范围2 000~3 000头/株的条件下,可获得较好的繁蜂效果,僵蚜的数量可达到8 000个/株以上。在繁蜂前期僵蚜的质量较高,僵蚜的羽化率可保持在90%以上,在繁蜂后期受重寄生蜂的影响,僵蚜的质量明显下降。应用田间小棚繁殖烟蚜茧蜂时,重寄生蜂是影响后期繁蜂质量的重要因素。