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91.
本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10%、15%、20%、25%和30%)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。 相似文献
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J Tetens S A Barker M Waguespack G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period. 相似文献
94.
Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls. 总被引:3,自引:0,他引:3
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M D Bierk S A Dee K D Rossow S Otake J E Collins T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266
The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals. 相似文献
95.
本工作用光镜和电镜技术对全沟硬蜱的围食膜进行了初步观察研究。结果表明,饥饿幼虫、若虫、成虫以及吸血期雄性成虫不具围食膜。吸血期幼虫、若虫及雌性成虫最晚分别于吸血开始后20、20及18小时出现了单层结构的围食膜;缓慢吸血期围食膜的厚度持续增加,而在快速吸血期其厚度却会显著减小。饱血后发育期围食膜不断增厚,愈来愈拱曲,与肠壁的距离不断扩大,并形成多层结构;至少分别于饱血后12、25及11天,仍能在幼虫、若虫及雌性成虫肠腔观察到完好状态的围食膜。全沟硬蜱是我国北方地区多种血液传播病原体的重要媒介,其围食膜的发现及围食膜形态变化的初步描述,对进一步认识这些病原体在全沟硬蜱体内迁移扩散规律、发育动态以及向宿主的传播途径,可提供有益的启示。 相似文献
96.
为解决整体叶轮扭曲叶片型面的精加工难题,本文进行了五轴联动数控展成电解磨削的基础研究.在分析平行直纹展成电解磨削整体叶轮扭曲叶片的过切误差、得到过切误差计算公式的基础上,提出用圆锥磨轮和组合式五轴联动数控方案来消除过切误差的方法.文中还介绍了数控展成电解磨床的结构与运动、经济型多轴数控系统及其联动控制方法,并建立了电解磨削非平行直纹展成曲面的数学模型,开发了五轴联动数控展成电解磨削自动数控编程系统,对航空发动机整体叶轮的叶片型面进行电解磨削.结果表明,加工效率比手工修磨、抛光提高了12倍以上. 相似文献
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99.
T D Butt A M Cruz J V Bailey W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939
The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti. 相似文献
100.
当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行. 相似文献