鸡Bcl-2基因的克隆及其对卵泡颗粒细胞凋亡的影响

将鸡的 Bcl- 2基因克隆到真核表达载体 JL V中 ,构建了重组质粒 JL VB。 0 .2μg/孔 JL VB重组质粒经脂质体介导转染卵泡颗粒细胞 ,应用流式细胞术等方法分析了转染 Bcl- 2基因后原代细胞和传代细胞的生长与凋亡情况。与对照组相比 ,转染后的传代细胞分裂速度较快 (P<0 .0 1) ,凋亡比率较低 ,G2 / M S期细胞比例极显著高于对照组 (P<0 .0 1)。这些结果表明 ,Bcl- 2基因具有促进细胞分裂、抑制细胞凋亡、延长细胞寿命的作用 ,这种作用是直接的。在转染时间为 12 h、DNA量为 0 .3μg/孔的条件下 ,较好的脂质体介导用量为 1.2 μL/孔。     

小麦新品种鄂恩5号性状表现及栽培要点

小麦新品种鄂恩5号集高产、稳产、多抗、广适于一体,分蘖力较强,成穗率高,穗层整齐,子粒饱满度好,农艺性状稳定,适宜长江中下游平原麦区单作或套作种植,在保证适时播种,密度合适,合理施肥,田间管理到位的条件下可获得高产。     

猪PCV-2和附红细胞体混合感染的实验报告

采取成都某猪场7头病猪料,经PCR检测后,有4头呈PCV-2阳性。以PCV-2阳性病料接种PK-15猪肾传代细胞,经分离和鉴定,获得PCV-2成都株。该猪场同时流行猪附红细胞体病,存在PCV-2和E．suis的混合感染。     

春秋兼用家蚕新品种“春华×秋实”的育成

以丝质优、耐氟污染性能较强的品种为育种材料,采用常规杂交育种方法,育成一对春秋兼用的中丝量家蚕新品种春华×秋实。浙江省蚕品种实验室共同鉴定和农村试养鉴定表明该品种具有以下特点:孵化、眠起、老熟齐一,体质强健好养,茧型较大,产量高;茧丝质优良,春期饲养茧层率23.46%,鲜茧出丝率17.54%,茧丝长1 263 m,解舒率83.52%,纤度2.773 dtex,洁净94.50分;综合经济性状优良,与对照品种菁松×皓月相比,万蚕产茧量提高7.0%,与对照品种秋丰×白玉相比,万蚕产茧量、万蚕茧层量、茧丝长、解舒丝长、鲜茧出丝率分别提高1.7%、8.9%、9.0%、6.6%、8.1%。新品种已通过浙江省农作物品种审定,适合在浙江省及长江中下游蚕区推广饲养。     

日本矮生沿阶草在长江中下游地区的引种适应性研究

日本矮生沿阶草(Ophiopogon japonicus(L.f.)Ker.-Gawler)是一种喜湿、耐荫、耐寒的地被植物, 具有耐贫瘠、适应性广、抗逆性强等特点,在园林绿化上有着广阔的应用前景。通过在长江中下游地区连续3年的引种栽培试验,表明日本矮生沿阶草在该地区有较强的适应性,本文还对其快速繁育技术进行了阐述。     

复方板黄口服液对畜禽常见病原菌的体外抑菌作用研究

为了研究板黄口服液对畜禽常见病原菌的体外抑菌作用,研究通过体外试验观察药物对禽巴氏杆菌、鸡葡萄球菌、鸡白痢沙门杆菌、肺炎克雷伯菌、牛巴氏杆菌、牛大肠杆菌的抑菌效果。结果表明:板黄口服液对鸡葡萄球菌的最小抑菌浓度(MIC)为125 mg/m L,对牛巴氏杆菌、禽巴氏杆菌、鸡白痢沙门杆菌、肺炎克雷伯菌、牛大肠杆菌的MIC均为250 mg/m L。说明板黄口服液对禽巴氏杆菌、鸡葡萄球菌、鸡白痢沙门杆菌、肺炎克雷伯菌、牛巴氏杆菌、牛大肠杆菌有很好的抑制作用。     

Evaluation of Therapeutic Effect of Porcine β-defensin-2 on Streptococcus suis Infection

Defensin is a small cationic peptide widely distributed in animals and plants. It is an important component of the innate immune defense system of mammals. It has a broad spectrum of antibacterial, antiviral and antifungal activities. Porcine β-defensin-2 (PBD-2) is one of the natural defensins expressed in pigs and has good antibacterial activity in vitro. The purpose of this study is to evaluate the therapeutic effect of PBD-2 in the treatment of Streptococcus suis infection in animals and to provide more insights for evaluating the value of PBD-2 as a therapeutic drug. Firstly, the bactericidal activity of PBD-2 against S. suis clinical isolate SC-19 was measured in vitro, and the cytotoxicity of PBD-2 was tested on mouse-derived macrophages (RAW264.7). Subsequently, C57 female mice aged 4-6 weeks and weighing between 18-22 g infected with S. suis SC-19 were treated with PBD-2 or PBS (n ≥ 6). The survival rate of mice, and the bacteria loads, inflammatory cytokine levels, and pathological changes of tissues were determined. The results showed that PBD-2 (25-200 μg·mL^-1) could significantly inhibit the growth of S. suis SC-19 in a concentration-dependent manner (P<0.05). At the same time, PBD-2 had no significant toxic effect on RAW cells (P>0.05). The in vivo study revealed that PBD-2 treatment could effectively reduce the mortality of mice infected with S. suis SC-19. At the same time, PBD-2 treatment significantly reduced bacteria loads in brain, lung, spleen and blood of mice, and decreased the levels of inflammatory cytokines IL-6, IL-12 and TNF-α in mouse sera (P<0.05). At the same time, PBD-2 treatment also significantly alleviated the degree of pathological damages in lung and spleen tissues of mice infected with bacteria (P<0.05). These results indicate that PBD-2 confers great resilience to S. suis in vitro without significant cytotoxicity, while it also exhibits a therapeutic effect on S. suis infection in mice, suggesting that the use of PBD-2 as a therapeutic drug and substitutes for antibiotics is of an excellent prospect.     

三峡水库重庆库区移土培肥的效益分析——以云阳县为例

应用前后对比分析法,对三峡水库重庆库区云阳县移土培肥一期与二期工程进行效益分析;得出移土培肥工程将给三峡库区带来巨大的生态效益、经济效益和社会效益的结论;说明该工程不仅能带动库区经济发展,并对其社会及生态环境产生深远影响。     

蒙农3号高丹草选育

从高梁雄不育系A3×黑壳苏丹草杂种F2代分离群体中开始选择优秀单株,通过4次单株选择、1次混合选择及品比、区域与生产试验,历经8个世代育成蒙农3号高丹草.该品种根系发达,茎基部节问短缩粗壮,株高约380cm,生育期141d,种子活秆成熟,分蘖多,再生性强,每年可刈割利用3次,鲜草产量和种子产量分别为13.30万kg/hm2和0.58万kg/hm2;茎叶柔嫩.茎叶比2.81,拔节期粗蛋白质含量13.77%.开花期粗蛋白质含量11.12%、粗脂肪4.07%、粗纤维36.11%、粗灰分5.36%、无氮浸出物41.67%,糖锤度3.86%.富含17种氨基酸.CN-含量低.适口性好,可青饲、青贮或晒制干草;植株抗倒伏,田间未发生病虫危害症状,适应性广,适宜在有效积温≥2400℃的地区推广种植.     

墨西哥柏育苗试验结果分析

切根及喷云大- 120 对墨西哥柏苗木生长的效果的试验结果表明: 切根和喷云大- 120 处理对墨西哥柏苗木生长的促进作用不大。在苗高、地径、冠幅等10 个性状中, 仅切根对茎叶鲜重呈明显负作用, 切根、喷云大- 120 及交互作用对根鲜重亦呈明显的负作用。在墨西哥柏育苗中不宜采用切根和喷云大-120 处理。