团聚丽瓢虫生物学特性及其对铁杉球蚜的捕食作用研究

研究了团聚丽瓢虫Adalia conglomerate的形态特征、生物学特性以及对铁杉球蚜的捕食效应。试验结果表明,团聚丽瓢虫1a发生1代,以成虫越冬。团聚丽瓢虫卵期、1、2、3、4龄、预蛹和蛹的发育历期平均分别是10d～15d,5d～11d,7d～11d,7d～11d,8d～16d,20d～30d,15d～18d。团聚丽瓢虫成虫和3龄幼虫可捕食所有龄期的铁杉球蚜,尤其喜欢捕食卵。捕食能力随着自身龄期增大而增大,成虫日最大捕食量最大,为344．8头。团聚丽瓢虫成虫和幼虫对铁杉球蚜卵的捕食效应均符合HollingⅡ型模型。成虫对铁杉球蚜的寻找效应用Hassel＆Varley模型进行模拟,模型为E=0．4517P^0.5568,r^2=0．9859,表明随着猎物密度的增加,个体间的干扰越强,表现出对铁杉球蚜的捕食作用减小。     

福建泉州清源山植被类型及演替趋势分析

据调查,泉州清源山区内有常绿阔叶林、针阔混交林、针叶林、灌丛、草丛草坡、沼泽草甸及人工植被等7个植被类型。本文对代表性的17个群落特征进行论述,并探讨植被的演替趋势。     

气相色谱法测定二嗪磷原药

本文采用毛细管气相色谱法测定二嗪磷的含量,以邻苯二甲酸二丁酯为内标物,采用丙酮溶解,使用HP-5,30m×0.32㎜×0.25μm,熔融石英毛细管柱和氢火焰检测器,对试样进行气相色谱分离和测定。实验证明,该方法简便快速,结果重现性好,定量准确。方法的准确度,精密度以及线性关系也比较好。     

Identification and mapping of a novel Turnip mosaic virus resistance gene TuRBCS01 in Chinese cabbage (Brassica rapa L.)

We aimed to identify Turnip mosaic virus (TuMV) resistance genes in Chinese cabbage by analysing the TuMV resistance of 43 P₁ (resistant), 88 P₂ (susceptible), 26 F₁, 104 B₁ (F₁ × P₁), 108 B₂ (F₁ × P₂) and 509 F₂ individuals. All parents and progeny populations were mechanically inoculated with TuMV‐C4. Both F₁ and B₁ populations showed TuMV resistance. Resistant: susceptible ratios in the B₂ and F₂ populations were 1 : 1 and 3 : 1, respectively. TuMV resistance in P₁ was controlled by a dominant gene, TuRBCS01. Bulked segregation analysis was performed to identify simple sequence repeat or insertion or deletion markers linked to TuRBCS01. Data from 108 B₂ individuals with resistant or susceptible phenotypes were analysed using mapmake r/exp 3.0. Polymorphic marker sequences were blast searched on http://brassicadb.org/brad/ . TuRBCS01 was found to be linked to eight markers: SAAS_mDN192117a_159 (3.3 cM), SAAS_mDN192117b_196 (4.0 cM), SAAS_mDN192403_148 (13.0 cM), SAAS_mGT084561_233 (6.8 cM), BrID10723 (3.3 cM), mBr4041 (3.3 cM), SAAS_mBr4055_194 (2.6 cM) and mBr4068 (4.0 cM). Further, TuRBCS01 was mapped to a 1.98‐Mb region on chromosome A04 between markers BrID10723 and SAAS_mBr4055_194.     

Application of improved genetic algorithm to locating and sizing of distributed generation

A Prüfer-coded genetic algorithm based on the decimal number of nodes is proposed and it is used to solve collaborative optimization planning of access solution of distributed generation(DG)and structure of distribution network.Using graph theory to generate theoretically feasible topology structureand the access nodes and the installed capacity of distributed generation are coded by the Prüfer number.The coding method makes the distribution network operation structure and the access solution of DG combine into the evolution problem of the same chromosomal gene.The length of the chromosome coding of this method is shorter than binary encoding.This solution takes full advantage of the Prüfer-coded to improve computational efficiency and convergence rateand makes some restrictions and improvements in some key parts of the algorithm to solve the problem illegal solution.Finallyfeasibility and superiority of the algorithm is validated by a case study.     

大花序桉种源间木材物理性质变异研究

以广西东门林场18年生的11个大花序桉(Eucalyptus cloeziana)种源木材为研究对象,对它们的木材基本密度、弦向干缩率、径向干缩率等物理性质进行了测定分析,发现不同种源间、相同种源不同树干高度间所测定的物理性质指标均存在显著的差异。木材基本密度最大的种源是B85,然后依次是D47、B82,其值分别是0.7530、0.7386、0.7274 g/cm3;参试的11个种源中有8个种源的差异干缩都小于1.5,属于差异干缩小的类型;只有14425、12195和B55这3个种源的差异干缩超过1.5,但小于1.6,属于差异干缩中等类型;种源的树高、胸径生长性状与木材基本密度、差异干缩、体积全干缩率物理性状相关关系不密切,据此分别对树高、胸径等生长性状和木材基本密度、干缩率等木材物理性状进行独立选择可望获得较好的生长、物理性状改良效果。     

北美红杉扦插生根的影响因素分析

研究了扦插基质,激素种类、浓度与处理时间,采条部位以及基因型对北美红杉扦插成活率的影响。结果表明:基质配比、激素种类、浓度及处理时间、采条部位等对北美红杉扦插成活率均有显著影响。基质配比以珍珠岩∶草炭=7∶3效果最好;激素以1 000 mg/kg ABT速蘸处理效果为佳;枝条顶部插穗好于下部插穗。不同基因型的扦插成活率差异显著。选取合适的基因型,采集顶部插穗,用1 000 mg/kg的ABT速蘸处理,扦插在珍珠岩∶草炭=7∶3的基质上能获得理想的效果。     

Exogenous carbon monoxide protects against the lung injury induced by ischemia-reperfusion of hind limbs in rats

AIM: To study the mechanism of protective effect of exogenous carbon monoxide (CO) in the lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. METHODS: Thirty-two SD rats were randomly divided into 4 groups: control, control+CO, IR and IR+CO. A rat model of ischemia in hind limbs and the reperfusion lung injury was made. The rats in IR+CO and control+CO groups were exposed to air containing 2.5×10-8 CO for 1 h before reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The lung tissue structure, polymorphonuclear leukocyte (PMN) count, wet-to-dry weight ratio (W/D), malondialdehyde (MDA) content and the animal survival rate were observed. The carboxyhemoglobin (COHb) levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting. RESULTS: Compared to control, the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly increased in IR group. Compared with the IR group, the blood COHb level was significantly increased and the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly decreased in IR+CO group. CONCLUSION: These data suggest that exogenous CO attenuate limb IR-induced lung injury by down-regulatiny ICAM-1 expression and suppressing PMN sequestration in the lung following limb IR in rats.     

吸收剂量、贮藏时间及贮藏温度对辐照牛肉中2-十二烷基环丁酮的影响

分析了辐照牛肉中辐解产物2-十二烷基环丁酮在不同的吸收剂量(0.5~10.0kGy)、贮藏时间(0-40d)及贮藏温度(-20℃~4℃)下的变化规律。采用硅胶层析柱-气相色谱/质谱联用技术萃取分离并检测。结果表明,2-十二烷基环丁酮的浓度与吸收剂量呈线性正相关,随着贮藏时间延长其含量减少,贮藏温度对其没有显著影响。利用2-十二烷基环丁酮可鉴定牛肉是否经过辐照,在已知贮藏温度和时间的条件下,可评估辐照牛肉的大约吸收剂量范围。