平和琯溪蜜柚柑橘黄龙病危害情况及防控对策

柑橘黄龙病[candidatus liberobacter asiaticum（jagoueix et al）]是危害柑橘生产最危险的一种传染病。其病菌能侵染柑橘属、枳属和金柑属中绝大部分柑橘品种，迄今尚未发现有高效抗病或免疫的品种。     

食用菊花扦插繁殖影响因素的初步研究

从基质选择、插穗长度、生根粉处理及食用菊花品种几个方面,对影响食用菊花扦插繁殖成活率的有关因素进行了研究。结果表明,珍珠岩是食用菊花扦插的首选基质;插穗长度为10 cm时生根情况较好;生根粉能够显著促进插穗生根;食用菊花插穗生根能力不同品种间差异较大。     

喷施6-BA和尿素对匍匐翦股颖种茎扦插成活率和生长的影响

对匍匐翦股颖粤选1号种茎采收前24 h和采收后分别进行不同浓度的6-BA(1.0、2.5、5.0、7.5 mg/L)和尿素(0.5%、1.5 %、2.5%)处理,清水对照(CK),探讨其对粤选1号匍匐翦股颖种茎扦插成活率和生长的影响.通过观测种茎的外观变化,种茎成活率、根长、生根数、根重、分蘖数、根系活力和叶绿素含量的的变化,初步确定种茎采前处理用2.5 mg/L 6-BA和2.5%尿素处理、采后用1.5%尿素处理均优于各自对照,但采前、采后处理中除根系活力与各自对照有显著差异(P＜0.05)外,其它指标均未达各自对照的显著水平;种茎采前与采后处理对比,采前处理效果总体好于采后处理.综上所述,种茎采收前选用2.5%尿素处理能明显提高种茎成活率和生根质量.     

Expression of IL-6 mRNA in endometrium with endometriosis

AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.     

mTOR kinase inhibitor CC-223 suppresses human breast cancer cell viability

AIM: To investigate the inhibitory effect of CC-223, an inhibitor of mammalian target of rapamycin (mTOR) kinase, on the viability of human breast cancer cells and its mechanism. METHODS: The inhibitory effect of CC-223 on the viability of MCF-7 cells and MDA-MB-231 cells was measured by CCK-8 assay. The cell cycle distribution of breast cancer cells was examined by flow cytometry. The expression of cell cycle-related proteins and oncoproteins c-Myc and survivin was analyzed by Western blot. RESULTS: CC-223 significantly inhibited the viability of both MCF-7 and MDA-MB-231 cells (P<0.05). CC-223 induced cell cycle arrest in both G₁ phase and G₂/M phase in the MCF-7 cells (P<0.05). However, low concentration of CC-223 treatment resulted in the accumulation of MDA-MB-231 cell cycle in the G₂/M phase, and the cell number in G₁ phase was unaffected. Treatment with CC-223 for 24 h clearly inhibited the protein levels of cyclin B1, cyclin D1 and phosphorylated cell division cycle protein 2 in the breast cancer cells (P<0.05). CC-223 suppressed the expression of c-Myc and survivin in both MCF-7 cells and MDA-MB-231 cells (P<0.05). CONCLUSION: CC-223 inhibits cell viability by blocking cell cycle progression and down-regulating expression of c-Myc and survivin in both MCF-7 and MDA-MB-231 cells.     

47份鲜食玉米对丝黑穗病和瘤黑粉病的抗性鉴定与评价

2018年通过田间人工接种对47份鲜食玉米杂交种进行了由丝孢堆黑粉菌(Sporisorium reilianum)引起的玉米丝黑穗病和玉蜀黍黑粉菌(Ustilago maydis)引起的玉米瘤黑粉病的抗性鉴定和评价。对丝黑穗病的鉴定结果表明,47份鲜食玉米杂交种中无高抗材料;4份材料表现抗病,分别是三禾甜加糯6号、金辉588、金辉895和黑甜糯520,占8.5%;1份材料盛彩甜3号表现中抗,占2.1%;14份材料表现感病,占29.8%;28份材料表现高感,占59.6%。对瘤黑粉病的鉴定结果表明,47份鲜食玉米杂交种中27份材料表现高抗,占57.4%;6份材料表现抗病,占12.8%;6份材料表现中抗,占12.8%;5份材料表现感病,占10.6%;3份材料表现高感,占6.4%。鲜食玉米新品种中抗丝黑穗病品种比较匮乏,抗瘤黑粉病品种比较丰富,但兼抗两种病害的品种严重缺乏。     

黄瓜果实弯曲相关基因Cs14-3-3 的克隆及表达分析

 以黄瓜（Cucumis sativus L.）果实易发生弯曲的品种‘长春密刺’为试验材料,采用RT-PCR 技术从果皮中分离并克隆了14-3-3 蛋白基因,并将此基因命名为Cs14-3-3。Cs14-3-3 基因cDNA 全 长792 bp,编码263 个氨基酸,分子量29 589.287 Da,等电点为4.585。生物信息学分析表明此基因含有 14-3-3 蛋白基因的典型结构域,与其他物种14-3-3 蛋白基因核苷酸序列同源性达到75%以上,编码的氨 基酸序列同源性达到85%以上,属于14-3-3 蛋白基因家族。同时,采用实时荧光定量PCR 方法对顺直果 实和弯曲果实腹部及脊部在果实开花的2、4、6、8、10、12 d 的基因表达量进行了研究。结果显示,在 果实发育各个时期,Cs14-3-3 的表达量均为在弯曲果实腹部 > 顺直果实 > 弯曲果实脊部的表达量;在 各个部位,Cs14-3-3 在果实开花2 d 的表达量明显高于开花后其他时期。试验结果表明,Cs14-3-3 基因为 黄瓜果实弯曲相关基因,在黄瓜果实开花早期发育过程中起重要作用。     

不同滴灌量对日光温室番茄生长、产量和品质的影响

为明确京郊日光温室番茄滴灌条件下适宜的灌溉量,以番茄‘欧盾’为试验材料,研究不同滴灌量对其生长、产量和水分生产效率的影响。结果表明:T1处理产量最高达到113 700 kg/hm2,较对照增产6.5%,果实品质有所提高,水分生产效率提高29.8%;其次是T2处理,较对照增产3.0%,水分生产效率提高54.4%;T3处理较对照产量有所降低。综合产量、品质和水分生产效率等指标,在该试验条件下,单次灌溉24 mm(T1处理)是温室番茄滴灌条件下较适宜的灌溉量。     

S100A6 promotes proliferation and migration of human osteosarcoma cell line 143B through PI3K/Akt signaling pathway

AIM: To investigate the effect of PI3K/Akt signaling pathway on S100A6-induced proliferation and migration of human osteosarcoma cell line 143B. METHODS: Recombinant human S100A6 protein (rhS100A6) was prepared. The 143B cells were treated with rhS100A6 in the presence or absence of PI3K inhibitor (LY294002 or wortmannin) exposure. The final concentrations of rhS100A6, LY294002 and wortmannin were 30 mg/L, 10 μmol/L and 0.5 μmol/L, respectively. The expression levels of total Akt (t-Akt) and phosphorylated Akt (p-Akt) in the 143B cells were analyzed by Western blotting. The cell proliferation and migration were determined by MTT and Transwell assays. RESULTS: rhS100A6 protein was successfully prepared, and significantly increased the proliferation and migration of 143B cells (P<005). rhS100A6 up-regulated the phosphorylation of Akt in 143B cells (P<005). Compared with rhS100A6 group, the level of p-Akt in 143B cells and the proliferation and migration of the cells were decreased in combined treatment group of rhS100A6 with LY294002 or wortmannin (P<005), where the proliferation rate at different time points dropped from 10.3% to 69.7% (P<005), and the migration rate dropped from 34.9% to 47.7% (P<005). CONCLUSION: To some extent, S100A6 promotes proliferation and migration of human ostersarcoma cell line 143B through PI3K/Akt signaling pathway.     

江苏中南部设施蔬菜盐渍化土壤盐分离子含 量及其垂直分布调查

为了解设施蔬菜土壤盐渍化状况,对江苏中南部设施蔬菜产区典型盐渍化土壤的0～40 cm
土层,以10 cm 为单位进行分层取样,并以相邻棚外的露地土壤为对照,测定了土壤EC 值及主要盐分离
子含量在土层间的垂直分布情况。结果表明：设施内各土层的EC 值和主要盐分离子含量均明显高于棚外
对照。阴离子以NO₃^- 为主,但SO₄^2- 含量也较高,阳离子则以Ca²⁺ 为主。设施土壤0～10 cm 土层的EC
值和主要盐分离子含量均较高,具有明显的表聚性;部分基地的土壤盐渍化已向深层发展。