中国荷斯坦牛GlyCAM1基因遗传多态性的研究

以273头中国荷斯坦牛为研究对象,利用CRS—PCR、PCR—SSCP及DNA测序技术检测了GlyCAM1基因外显子3、内含子3的遗传多态性。结果表明：GlyCAM1基因分别在外显子3和内含子3的第2081（A／C）、2417（C／T）位存在突变,2个位点的等位基因频率A／B分别为0．7525,0．2475和0．9046,0．0954;经菇。适合性检验,中国荷斯坦牛内含子3的突变达到Hardy—Weinberg平衡状态（P〉0．05）,但其外显子3的突变未达到Hardy—Weinberg平衡状态（P〈0．05）。     

Effects of Momordica grosvenori Saponins on Type 2 Diabetes Rats Induced by High-sucrose/High-fat Diet and Streptozotocin

The purpose of the experiment was to study the effect of Momordica grosvenori saponins on improving the effect of high-sucrose/high-fat diet combined with streptozotocin induced type 2 diabetes in rats.The type 2 diabetes rat model was established with a high-sucrose and high-fat diet combined with streptozotocin.Successfully modeled rats were given the total saponins of Momordica grosvenori (100,200,400 mg/kg) or metformin (268 mg/kg) by gavage,once a day for 4 weeks.Recording the "three more and one less"index (24 h internal urine output,water intake and feed intake,body weight);Detecting of biochemical indicators,analysis of fasting blood glucose (FBG),serum total cholesterol (TC),triglycerides (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),insulin (INS),catalase (CAT),superoxide dismutase (SOD),malondialdehyde (MDA),alanine aminotransferase (AST),aspartate aminotransferase (ALT),blood urea nitrogen (BUN) and creatinine (Cr) levels.Hematoxylin-eosin staining (HE) was used to observe the pathological changes of the pancreas.Compared with the model group,the feed intake,water intake,urine output,blood indicators FBG,INS,MDA,TC,TG,BUN and Cr levels,AST and ALT activities were significantly or extremely significantly decreased in the total saponins of Momordica grosvenori each dose group and metformin group (P<0.05;P<0.01),while CAT,SOD activities and HDL-C level were significantly or extremely significantly increased (P<0.05;P<0.01).In pancreatic tissue,the number of pancreatic cells was increased significantly,and the pathological damage of the pancreas was significantly reduced. Momordica grosvenori saponins and metformin had obvious effects on improving glucose metabolism disorders,and at the same time could significantly reduce insulin resistance,enhance the body’s ability to resist oxidative stress and improve liver and kidney functions.     

旅游解说牌示规划设计方法与技术探讨

针对目前我国旅游景区管理人员对旅游解说牌示的价值、规划方法与技术认识不深的现实,在旅游解说规划中充分体现人本主义的思想下,结合景区旅游解说资源的特性与游客特征,分析出旅游解说牌示规划与设计应包括规划阶段、设计阶段、建造阶段、评估与保持等4个阶段和组建解说队伍、清查解说资源、决定解说目标、选择牌示类型、选择牌示材料、连接对象与文本、确定牌示文本、确定牌示尺度、选择印刷方式、评估解说牌示与保持解说牌示等11个具体步骤.     

工农业用凿岩系统冲击机构建模与仿真

工农业实践中一般将冲击机构分为轻、重冲击工作状态。为了解决在荒山开发、矿山开采和山区土地开垦等过程中凿岩机的卡钎、偏斜问题,以WF100型液压凿岩机冲击机构为研究对象,建立系统仿真模型。通过仿真获得液压凿岩系统在重冲击、轻冲击2种工作状态下的工作性能参数。重冲击工作状态下,节流孔等效直径为7 mm,系统压力为12 MPa时,液压凿岩机冲击能达到77 J;节流孔等效直径为7 mm,系统压力为14 MPa时,液压凿岩机冲击能达到98.3 J。轻冲击工作状态下,节流孔等效直径为2.5 mm,系统压力为12 MPa时,液压凿岩机冲击能达到42.5 J;节流孔等效直径为2.5 mm,系统压力为14 MPa时,液压凿岩机冲击能达到53 J。研究结果为冲击结构的设计提供了参考,也可为液压凿岩机在不同状态下工作时保证凿岩工作效率又避免卡钎提供技术指导。      

超级稻专用肥对不同种植方式的适应性研究

为了探明超级稻专用肥对不同种植方式的适应性,以常规超级稻合美占为材料,通过大田试验,研究了施用超级稻专用肥(SSF)对人工插秧、机插秧、撒直播、免耕撤直播、机穴直播、抛秧、免耕抛秧7种种植方式下水稻产量和品质的影响.结果表明:与施用常规化肥(CK)相比较,SSF显著提高人工插秧、机插秧、撒直播、免耕撒直播、机穴直播、抛秧、免耕抛秧7种种植方式的产量,分别提高20.03％、12.36％、22.26％、9.2％、22％、21.93％和17.56％; SSF亦能显著提高人工插秧和机插秧稻米的胶稠度,以及撒直播稻米的蛋白质含量,而显著降低了抛秧稻米的垩白粒率和垩白度.超级稻专用肥对上述7种种植方式均具有良好的适应性.     

野蔷薇种子休眠和萌发整齐度研究

野蔷薇的种子低温砂藏30 d才开始发芽,然后再需50d时间才能结束发芽,发芽率为75％左右.酸处理、酸蚀裂口处理可缩短种子低温砂藏时间和发芽时间;通过清水分级可剔除劣质种子,提高种子质量的一致性,从而提高发芽率和发芽整齐度.将野蔷薇种子用盐水分级,剔除劣质种子,然后将分级种子用浓盐酸处理20 min,制成酸蚀种子,再经裂口处理,制成分级酸蚀裂口种子,则处理种子低温层积13 d开始发芽,发芽时间缩短为16d,发芽率提高到90％以上.     

草莓新品种"红颊"的生产适应性研究

通过在贵阳市、安顺市、麻江县3个试验点的试验,将红颊与鬼怒甘进行对照比较,分析上市期、产量、品质。结果表明：红颊在贵州省海拔700～1300m的地区均适宜大棚栽培,其产量与鬼怒甘相当,采收期提前20天左右,风味、可溶性固形物含量优于鬼怒甘,适宜在我省作促成栽培品种推广。     

基于灰色关联下的广西粮食主要生产影响因素评价

通过对1995—2010年主要年份广西主要粮食（稻谷、玉米、大豆、薯类）产量与相关生产因素的灰色关联度动态分析,提出广西主要粮食生产的可持续发展途径,为农业可持续发展提供参考。运用灰色系统理论中的关联度分析法对1995—2010年其中6个主要年份与粮食生产有关的7个影响因素进行量化动态分析。结果表明：粮食产量与相关生产因素的关联度为播种面积〉城镇居民家庭人均购买粮食数量〉化肥施用量〉乡村从业人员〉农田有效灌溉面积〉单位面积产量〉农田总用电量。     

Influence of MUD on the System Capability of CDMA

In CDMA communication system, the relativities between users result in Multiple Access Interference (MAI). With the increase of users, MAI becomes the main jam of broadband CDMA communication system. Multi-User Detection (MUD) is the most important technology of anti-jamming in the broadband CDMA communication system, which can eliminates MAI effectively by using the information of all user signals to detection single. This paper analyze the expression of system capability without MUD and with MUD, and puts forward the way to increase system capability by using MUD. Then the influence of MUD on CDMA system capability is discussed by MATLAB emulation. Basing analysis and emulation, the conclusion is got: the higher the MUD efficiency, the better the improvement of CDMA system capability; under the same MUD efficiency, the lower data rate, the smaller inter-cell interference, the lower the ratio of bit energy to power spectrum, the better the improvement of CDMA system capability.     

柑橘新品种‘粤丰早橘’

‘粤丰早橘’是从‘南丰蜜橘’芽变中选育出来的优良新品种,具有适应性强,丰产稳产、果实大、化渣性好等特点,平均单果质量58.33 g,可溶性固形物含量13.36%,总糖含量11.80%,总酸含量0.52%。在广东韶关地区种植,果实10月上旬成熟。