Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

微卫星DNA在动物亲子鉴定中的应用及研究进展

本文概述了微卫星DNA进行亲子鉴定的基本原理与方法,将其用于亲子鉴定的优点以及研究进展,最后对未来的应用进行了展望。     

Analysis of High Reproductivity on Wild Yak Bull

Sperm concentration of wild yak bull is 2. 13 billion per milliliter. Survival time at 0-4 ℃ is 57hrs. After thawing survival time at 37 ℃ is 12 hrs. Resistance coefficient is 144,000. Abnormal sperm rate and acrosomal integrated rate of post-thawing is 9.17% and 87.53% respectively. Moving viscosity is 1. 169 cp. Total nitrogen is 1 437.7 mg/100 ml. Head of sperm is significantly shorter and end piece is significantly longer than that of domestic yak and yellow cattle. Activity of hyaluronidase is highly stronger than the domestic yak,and the activity of LDH is higher than the domestic yak by 48 %. Hence,the fertility of the wild yak bull is powerful and the artificial inseminated rate with domestic yak and yellow cattle is 88.9% and 71.58% respectively.     

植物Ca2+信号的研究进展

在植物的生长发育过程中,为了适应环境,调节自身代谢和生长,需要对各种外界环境刺激以及植物内部生理信息做出反应,因此,植物产生了自己的信号系统。Ca2 作为一种信号分子在植物细胞信号系统中起着举足轻重的作用。针对国内外对植物Ca2 信号的研究情况,综述了Ca2 信号的产生、Ca2 信号参与的各种植物生理过程、Ca2 信号的检测以及其研究的最新进展。     

水稻无盘旱育免耕抛秧不同密度试验

为了探索水稻无盘旱育免耕抛秧适宜栽培密度和技术,设计了6种不同抛秧密度的试验。结果表明,性状指标变化情况为:株高、穴穗数、穗长、穗总粒数、穗实粒数随抛秧密度的增加而减少,667m2穗数随抛秧密度的增加而增加,够苗期随密度的增加而提前,其他性状指标变化不大。667m2抛秧14 000、17 000和20 000粒谷秧的处理4、5、6产量较高,分别为760.3 kg/667m2、718.2 kg/667m2和712.8 kg/667m2,与其余3个处理间达显著或极显著水平。无盘旱育免耕抛秧适宜的密度范围为1 4 000~20 000粒谷秧/667m2。     

百宜黑羽鸡外貌特征及生长发育研究

从选育中的1,2世代百宜黑羽鸡的外貌特征、生长性能两个方面对百宜黑羽鸡的部分特征特性进行了研究,结果表明:百宜黑羽鸡具有全黑羽带墨绿色(脚、胫、喙、耳毛为黑色),体形中等,肤白色。2周龄时公母均出现日增重率先升后降,分别由0.048%和0.48%上升到41.72%和37.79%,接着又下降到5.69%和5.06%。百宜母鸡的生长曲线从10周龄开始与公鸡的生长曲线分离,17周龄后母鸡的生长速度明显慢于公鸡。     

提高仔兔成活率的技术管理措施

从出生到断奶这段时间的兔称为仔兔,根据其生理特点,此阶段可分为睡眠期和开眼期,这是仔兔生长发育过程中非常重要而又很难饲养的时期.在养兔生产中,仔兔成活率的高低对经济效益有很大的影响,直接关系到养兔的成败.因此,加强仔兔饲养管理,采取有效的保护措施,提高仔兔成活率是养兔成功的关键.     

紧凑型杂交玉米新品种毕玉2号的选育与应用

毕玉2号系毕节地区种子站用自选系苏110作母本,自选系毕106作父本育成的白粒紧凑型玉米杂交种,2000年11月通过贵州省毕节地区农作物品种审定委员会审定,具有较强的耐旱耐瘠能力和品质优良等特点,适宜在毕节地区海拔1 800 m以下及其他气候相似地区的旱坡地及肥水条件好的坝地种植,目前正在生产上大面积推广应用。