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191.
WU Xue-lei CAI Yao-wu ZHUANG Zhi-zhong CHEN Yuan-jing GUO Ren-jie ZHENG Mao-song 《园艺学报》2015,31(12):2169-2175
AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression. 相似文献
192.
WU Xiao-hu XU Jun LIU Yong-zhuo DONG Feng-shou LIU Xin-gang ZHANG Wen-wen ZHENG Yong-quan 《中国农业科学(英文版)》2015,(1):114-124
The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested. 相似文献
193.
XIAO Sui FEI Chen-zhong ZHANG Li-fang ZHENG Wen-li ZHANG Ke-yu XUE Fei-qun 《中国农业科学(英文版)》2014,(5):1082-1089
Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography (HPLC) fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time. 相似文献
194.
信息技术给高校图书馆的发展带来了机遇和挑战,信息资源已经成为高校图书馆馆藏资源的重要组成部分。从理论、观念、服务模式等方面阐述了信息时代高校图书馆信息资源建设应采取的措施。 相似文献
195.
浅谈大田县政策性水稻种植保险现状及对策 总被引:1,自引:0,他引:1
大田县开展政策性水稻保险工作取得显著的成效。该文总结了大田县推进政策性水稻种植保险的意义、现状、存在问题,并提出今后完善政策性水稻种植保险的对策。 相似文献
196.
针对氧化铝蒸发工序中出口浓度检测滞后的现状,建立氧化铝生产逆流多效蒸发的动态机理模型。采用常微分方程组的MATLAB工具箱函数解法,并对第五、六效蒸发过程进行仿真研究。结果表明,该模型能够较好地逼近实际系统,并对出口浓度的在线实时监测有现实的指导意义。 相似文献
197.
198.
郑开 《湖南农业大学学报(自然科学版)》2016,(4):59-66
老庄哲学语境中的“德”含义复杂且深邃,比如说“玄德”概念及其理论创造性地发展了由来已久的德的观念,可谓思想史进程中“哲学突破”的重要标尺。围绕着老庄哲学的“德”,有若干问题值得深入探究:第一,道与德之间的关系能否归结于“德者,得也”的训诂解释,或者说道与德之间是否有那种亚里士多德意义上的分有关系?如果考虑到“德”实际上意味着“性”或者更深意味的“性之性”,那么德就具有两层含义:万物殊性且自得其性的性;万物玄同意义上的“性之性”。第二,老庄往往通过宇宙论模式阐发道物关系,同时也展现了探究德与物之间关系的多元思考,后者隐含了一种心性论的旨趣。第三,作为价值判断语词的“德”,足以提示出超越于伦理规范的原初伦理,即更高理论层次的德性伦理学(含政治哲学)。这几个问题的新思考有助于加深对老庄哲学的理解。 相似文献
199.
基于宁德市水利局、水文局、气象局等部门观测数据,建立防汛指挥信息集成掌上平台,为防汛指挥提供决策服务。平台建设涉及跨部门、跨网段、跨地域。文章重点介绍平台的实现难点:使用远程处理框架技术(.net Remoting)方便地解决部门间分布式数据库的命令传递;采用C#实现数据收集模块。最后,文章介绍了防汛平台功能特点。 相似文献
200.
The test was aimed at determining the effects of adding MOS to diets with different concentrate to forage ratios on ruminal fermentation of sheep in vitro.The 4×6 two-factor experimental design was chosen.There were 4 diets with different concentrate to forage ratios(20:80, 30:70, 40:60 and 50:50) and added to 6 dosages MOS(0, 0.4%, 0.8%, 1.2%, 1.6% and 2.0%), respectively.The method of gas production in vitro was used to measure the total gas and CH4 production, IVDMD, IVCPD and IVOMD after cultured for 3, 6, 9, 12 and 24 h.The results showed that the gas(except 12 and 24 h) and CH4 production(except 9 h) were not impacted by the two factors(P>0.05).The IVDMD, IVCPD and IVOMD were significantly affected by concentrate to forage ratios(P<0.05), while the IVCPD at 24 h was significantly affected by MOS(P<0.05).Meanwhile, all the indexes were not influenced by the interaction of two factors(P>0.05).The IVDMD and IVOMD increased with the rising of concentrate approximately, while the IVCPD decreased with the rising of MOS roughly.The biggest associative effect was gained in 30:70 concentrate to forage diet when adding 1.2% MOS. 相似文献