Effects of bilirubin on oxygen free radicals and caspase-3 in acute lung injury rats

AIM:To investigate the mechanisms by which bilirubin inhibits acute lung injury (ALI). METHODS:30 female Wistar rats were divided into normal group, ALI group and bilirubin treatment group. ALI was induced by intravenous injection of LPS. The contents of OH^-, H₂O₂ and O₂^· in the lung as well as the expression of caspase-3 in the lungs were investigated. RESULTS:(1) The contents of OH^-, H₂O₂ and O₂^· in the lung homogenate and the expression of caspase-3 in the lungs in ALI group increased compared with those in normal group (P<0.05). (2) The contents of OH^-, H₂O₂ and O₂^· in the lung homogenate and the expression of caspase-3 in the lungs in bilirubin treatment group increased compared with those in normal group, but decreased compared with those in ALI models (P<0.05). CONCLUSION:(1) Bilirubin was shown to be able to ameliorate apoptosis in ALI rats. (2) The increase in the contents of OH^-, H₂O₂, O₂^· in ALI group indicated the development of oxidative lung injury, which was ameliorated by bilirubin. (3) Expression of caspase-3 confirmed that the model made by LPS was associated with apoptosis, which was reduced by bilirubin.     

Overexpressed human FAT10 protein induces the apoptosis in the starved HEK293 cells

AIM: To study the effect of human FAT10 on the apopotosis of HEK293 cells using flag-tagged human FAT10 protein.METHODS: The fragment of FAT10 gene was cloned into the pcDNA3-flag vector,which was identified by PCR,enzyme digestion and sequencing.The reconstructed plasmids were transfected into HEK293 cells.The expression of introduced FAT10 in the normal cultured and starved cells was detected respectively by Western blotting.XTT assay and DNA ladder method were used to analyze the effect of FAT10 on the apoptosis of starved HEK293 cells.RESULTS: The reconstructed plasmids were highly expressed in HEK293 cells with different expression mode at the mormal cultured and starved state.The livability of starved FAT10 overexpressed HEK293 cells was significantly lower than that of normal cultured cells.DNA ladder was observed in the starved FAT10 overexpressed cells,but not in the normal cultured cells.CONCLUSION: The eukaryotic expressed plasmids of flag-tagged FAT10 were constructed successfully,and highly expressed in HEK293.Overexpressed FAT10 enhances the apoptosis in the starved HEK293 cells.     

内蒙古景天属植物分类及其区系生态地理分布研究

本文试图澄清内蒙古究竟有多少种景天属植物,这些植物在内蒙古的生态分布情况。我们应用形态—地理分类的方法,对内蒙古景天属Sedum植物进行认真研究。研究认为:《内蒙古植物志》第二版记载的北景天(Sedum kamtschaticum Fisch.)和兴安景天(Sedum hsinganicumChu.)应为费菜;确定了兴安景天(Sedum hsinganicum Chu.)和费菜(Phdeimusaizoon L.)为温带亚洲分布种;阔叶景天(Sedum roborowskii Maxim.)为青藏高原东部分布种;藓状景天(Sedum polytrichoides Hemsl.)为中国-日本分布种;内蒙古景天属植物从东部渗入了1种东亚成分,从西南部渗入了1种青藏高原成分。     

中华真地鳖抗菌物质的抑菌活性测定

以研磨法从中华真地鳖五龄若虫体内提取得到抗菌物质,分别采用比浊法和菌碟法测定了其对多种细菌和植物病原真菌的抑菌活性。结果表明,该抗菌物质对大肠杆菌、金黄色葡萄球菌、白菜软腐菌和枯草芽孢杆菌等4种细菌生长均有一定的抑制作用,对数期延迟1~4h,菌的浓度、繁殖速度减缓。对7种植物病原真菌的抑菌试验表明,该抗菌物质对玉米茎基腐病菌、小麦根腐病菌、棉花枯萎病菌抑制效果较好,抑制率分别为50.0%、49.2%、45.2%;而对白菜黑斑病菌和玉米大斑病菌活性较差,抑制率仅为28.3%和24.5%。     

一种促进植物根系生长的极细链格孢菌蛋白质分离、纯化和生物功能

通过硫酸铵沉淀、有机溶剂沉淀、离子交换和分子筛连用的方法，从极细链格孢菌JH505菌株中分离纯化到分子量约为35kD的植物激发子。利用固相梯度凝胶双向电泳方法测定了该蛋白的等电点为4．22。将该纯化蛋白稀释液浸泡小麦种子8h，7d后小麦根系琥珀酸脱氢酶活性提高65．27％，经蛋白处理的小麦根长比对照提高13．1％。     

卷叶螟绒茧蜂寄生棉大卷叶螟的行为及其与寄主密度的关系

卷叶螟绒茧蜂Apanteles derogatae Watanabe是棉大卷叶螟Sylepta derogata Fabricius幼虫期的一种重要内寄生蜂,对棉大卷叶螟种群有较好的控制作用。在实验室条件下,运用人工接虫观察法研究卷叶螟绒茧蜂对棉大卷叶螟幼虫的寄生行为。结果表明,该蜂搜寻寄主的时间平均为2．38 min,处理寄主的时间仅需0．86 min;寄生寄主的最适龄期为寄主2龄幼虫;对1龄幼虫的致死率达32%,但其寄生率仅为16%,其余16%的个体为穿刺致死。饲喂蜂蜜水能显著增加该蜂的产卵量,延长其产卵历期和增强其穿刺能力。寄生蜂的寄生能力与寄主密度的关系呈抛物线型:在寄生蜂均为16头/m~3雌蜂条件下,当寄主密度从80头/m~3增加到320头/m~3时,寄主的被寄生个体数随之增加,但当寄主密度超过320头/m~3后,寄主的被寄生个体数反而随寄主密度的上升呈下降趋势。     

油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用

 从油菜植株体内分离出的内生细菌BY-2,经过生物学鉴定为枯草芽孢杆菌(Bacillus subtilis)。用BY-2回接油菜,重新分离到具有BY-2相同形态特征和抑制病原真菌能力的内生菌株。油菜接种后的第10 d,体内的BY-2菌数达(2.24~9.02)×10³ cfu/g鲜植株,25 d仍然保持在(3.13~8.59)×10³ cfu/g鲜植株。BY-2与油菜核盘菌[Sclerotinia sclerotiorum(Lib.) de Bary]对峙培养可以形成直径为3.1 cm的抑菌圈;可使油菜核盘菌菌丝细胞浓缩变短,细胞壁破裂,原生质外溢,从而抑制真菌生长发育;同时还能抑制菌核的萌发,抑制率达60%~70%;在油菜离体叶片试验中,BY-2对菌核病的防治效果达100%。     

逆固相基质分散净化和气质联机检测土壤中的六氯苯残留

建立了逆固相基质分散净化和气质联机法(GC-MS)快速、简便测定土壤中六氯苯(HCB)残留量的方法。以丙酮-正己烷(1∶ 1,体积比)为提取剂,采用超声波萃取,经固相基质PSA(primary secondary amine)吸附净化后采用GC-MS选择离子模式检测。结果表明,六氯苯在0.05~5 mg/L浓度范围内呈现出较好的线性关系,相关系数R2=0.999 5; 方法检测限为0.3 μg/kg,定量限为0.9 μg/kg,在0.89、1.78、8.90 mg/kg 3个添加水平上的平均回收率分别为106.6％、108.2％和108.1％。用该方法测定了天津某生产厂厂区地下1~10 m深的土壤样本,发现六氯苯的残留量在2.3~25 912 mg/kg 之间,表现出显著的空间变异趋势。该方法快速、简便、经济、高效、实用并且安全,适合大批量样本中六氯苯的快速检测。