全文获取类型
收费全文 | 177536篇 |
免费 | 9812篇 |
国内免费 | 17262篇 |
专业分类
林业 | 12891篇 |
农学 | 11105篇 |
基础科学 | 8970篇 |
19048篇 | |
综合类 | 83898篇 |
农作物 | 11916篇 |
水产渔业 | 6897篇 |
畜牧兽医 | 27718篇 |
园艺 | 13390篇 |
植物保护 | 8777篇 |
出版年
2024年 | 1496篇 |
2023年 | 3661篇 |
2022年 | 8132篇 |
2021年 | 8178篇 |
2020年 | 7493篇 |
2019年 | 7581篇 |
2018年 | 5548篇 |
2017年 | 8253篇 |
2016年 | 5600篇 |
2015年 | 8694篇 |
2014年 | 9237篇 |
2013年 | 10755篇 |
2012年 | 14841篇 |
2011年 | 15351篇 |
2010年 | 14862篇 |
2009年 | 13169篇 |
2008年 | 12936篇 |
2007年 | 11815篇 |
2006年 | 9610篇 |
2005年 | 7488篇 |
2004年 | 4773篇 |
2003年 | 3104篇 |
2002年 | 3073篇 |
2001年 | 2918篇 |
2000年 | 2607篇 |
1999年 | 1157篇 |
1998年 | 335篇 |
1997年 | 305篇 |
1996年 | 229篇 |
1995年 | 219篇 |
1994年 | 201篇 |
1993年 | 171篇 |
1992年 | 165篇 |
1991年 | 149篇 |
1990年 | 88篇 |
1989年 | 74篇 |
1988年 | 60篇 |
1987年 | 57篇 |
1986年 | 46篇 |
1985年 | 10篇 |
1984年 | 12篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 29篇 |
1965年 | 3篇 |
1963年 | 1篇 |
1962年 | 39篇 |
1958年 | 1篇 |
1956年 | 49篇 |
1955年 | 23篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
苏云金芽孢杆菌cry基因在大肠杆菌中表达产物和生物活性 总被引:4,自引:0,他引:4
研究了几种Bt cry基因于大肠杆菌(Escherichia coli)中表达产物在pH 10.0的50mmol/L碳酸钠和20mmol/L乙醇胺溶解液中的溶解性 ,发现同样的Cry蛋白在碳酸钠中的溶解度大于乙醇胺。通过胰蛋白酶消化 ,明确Cry1Ca7、Cry1Ia8酶解产物为 38kD多肽 ;Cry1Ie1、Cry1Cb2、Cry2Ab4酶解产物为 41kD多肽 ;Cry1Ac酶解产物为60kD多肽。采用FPLC层析方法对 6种原毒素及其酶解后得到的毒素多肽进行了分离纯化 ,比较了原毒素和毒素的杀虫活性的差异。其结果表明 ,Cry1Ac的原毒素和毒素对棉铃虫初孵幼虫的校正死亡率均为 100% ,Cry2Ab4的原毒素的毒力高于其酶解毒素。 相似文献
52.
AIM: To prepare gfp-bcl-XL-contained recombinant adenovirus(rAd-gfp-bcl-XL).METHODS: Bcl-XL gene was amplified from pEGFP-C3-bcl-XL, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-XL. Then pAdTrack-CMV-bcl-XL was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-XL and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-XL into 293 cells. PCR test indicated that the recombinant Ad contained bcl-XL gene. The titer of the purified rAd-gfp-bcl-XL was 6.5×1012 PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-XL. This affords a good gene transfer vector for the gene therapy in human’s diseases. 相似文献
53.
ZHANG Li-rong XIANG Peng XIA Wen-jie CHEN Zhen-guang ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2002,18(8):896-899
AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK. 相似文献
54.
CHEN Xiao-yin ZHANG Qun YANG Qin-he XIE Qiu-ling SHEN Qiang XU Yun-sheng 《园艺学报》2002,18(12):1529-1531
AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level. 相似文献
55.
56.
57.
58.
Dose response of weeds to methyl iodide and methyl bromide 总被引:1,自引:0,他引:1
W. M. ZHANG M. E. McGIFFEN JR J. O. BECKER H. D. OHR J. J. SIMS R. L. KALLENBACH 《Weed Research》1997,37(3):181-189
Labonuory bioassay and field experiments were conducted to characterize the dose response of weeds to methyl iodide and methyl bromide as soil fumigants. The patterns in potency of both fumigants and in sensitivity of diffcretit weed species to the fumigants were distinguished with the use of logistic dose-response models. Similar to its response to methyl hromide fumigation. Amaranthus retrofleus L. was the most sensitive to methyl iodide fumigation. Cyperus rotundtis L. was the least sensitive to methyl iodide fumigation, whereas Portuloca oleracea L. was the least sensitive to methyl hromide. Lolium multiflorum Lam. Abutilon theophrasti Medik. Chenopodium album L. P. ateracea . Brassica kaber (D.C.) L.C. Wheeler and Cyperus escuden-tus L. were similar in sensitivity to methyl iodide. Methyl iodide was as potent as methyl bromide for A. retroflexus but more potent than methyl bromide for L. multiflorum , A. theophrasti. C. album. P. oleracea. B. kaber, C. esculentus and C. rotundus. The dose response for weeds in the field was similar to that obtained in laboratory bioassays. Under fieid conditions. 280 kg ha-1 methyl iodide killed all species tested except Solanum nigrum L Methyl iodide appears to be a suitable replacement for meihyl bromide because it can be used in situations simitar to methyl bromide fumigation, has superior efficacy against a broad spectrum of pests and has a low potential for degrading the earth's ozone lavers. 相似文献
59.
大豆疫病的种子处理技术研究 总被引:2,自引:0,他引:2
本试验用不同浓度的瑞毒锰锌、杀毒矾和克露进行大豆种子处理试验,结果表明,500ppm瑞毒锰锌对大豆疫病具有很好的治疗作用,而用杀毒矾以种子重量0.4—0.5%的剂量闷种,能显著地抑制大豆疫霉菌的侵入 相似文献
60.