不同去雄方法诱导草地早熟禾雄性不育的研究

试验分别用温汤去雄杂交和两种化学去雄剂化学去雄诱导草地早熟禾雄性不育,结果表明,在两种化学去 雄剂作用下去雄效果比较理想,温汤去雄雄性不育率达100％。     

高寒牧区牧鸡鸭治蝗效果试验

在青海湖北岸高寒牧区利用人工饲养的鸡鸭进行放牧防治草原蝗虫。结果表明,在60d的防治期内,牧鸡鸭平均灭治效果达91.4%,为人工利用天敌治虫技术在高寒牧区的应用提供了依据。     

香菇出口生产技术综述

香菇无公害生产全程技术监控，包括优化产地生态环境栽培原材料把关，精选优良菌种，菌袋生产规范化，出菇科学管理，控制农药使用，产品标准化等七个方面。     

红星苹果和肥城桃果实软化的酶学基础

以红星苹果穴Malusdomesticacv.Starking雪和肥城桃穴Prunuspersicacv.Feicheng雪果实为试材,结合乙烯利和1-甲基环丙烯(1-MCP)处理,分别研究了2者软化的酶学基础,并就其差别作了探讨。结果表明:红星果实软化中起主要作用的酶为内切和外切多聚半乳糖醛酸酶(endo-和exo-PG);肥城桃果实软化的前20d则以纤维素酶(EGase)为主,20d后软化以endo-PG为主。研究同时发现,脂氧合酶(LOX)在这2种果实软化的启动阶段都起了明显的促进作用。1-MCP通过对红星果实中PG、肥城桃果实贮藏前期EGase和后期endo-PG活性的抑制,以及对2者软化启动阶段LOX活性的抑制,延缓了果实硬度的下降。     

新疆石河子地区沙漠增温效应对绿洲农业影响的研究

由于沙漠 ,绿洲 ,空气的比热等热性能不同 ,形成沙漠夏季温度明显高于绿洲 ,进而对绿洲地区增温 ,称沙漠增温效应。石河子地区 1 0 0 km范围内沙漠对绿洲的增温平均 1 .76℃ ( 1～ 2℃ ) ;根据绿洲距沙漠的远近与气温间存在的线性关系 ,回归方程为 :y=2 7.5 2 97- 0 .0 335 x。 r=- 0 .881 3*。沙漠增温效应对绿洲农业有多方面作用 ,形成夏季富照高温、秋季大日较差以及促成棉花在夏季的“冷害”等 ,深刻地影响绿洲特色农业、栽培技术及人们生活     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

塔里木河流域生态环境恶化的水文效应

塔里木河是我国最大的内陆河。 4条源流出山口多年平均天然径流量 2 2 4 .9× 10 8m3 (195 7～ 2 0 0 1年 )。在全球变暖的大背景下 ,塔里木河流域山区气候出现变暖增湿的趋势 ,2 0世纪 90年代径流量达 2 4 1.9× 10 8m3 ,增幅 7.6 %。由于源流区人类开发利用水资源的影响 ,使得补给塔里木河干流的水量不断减少 ,水文条件改变 ,造成干流上、中游段耗水严重 ,导致下游生态与环境急剧退化。自 2 0 0 0年以来 ,实施的从开都河—博斯腾湖向下游绿色走廊应急输水 ,已使下游生态环境开始恢复生机。     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts

AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK.     

利用RT-PCR与PVP-ELISA检测水稻瘤矮病单头叶蝉

 目前国内外对水稻瘤矮病毒(Rice gall dwarf virus,RGDV)的研究主要集中在其核衣壳蛋白和外衣壳蛋白的性质、结构及其与介体的亲和性、与介体传毒能力的关系等方面,本室在已有室内检测研究的基础上,利用RT-PCR和PVP-ELISA法对田间带毒叶蝉进行检测,并对2种方法的检测结果进行了比较。