沙门菌鞭毛基因表达的相变换研究进展

沙门菌是目前研究较多的细菌之一,广泛分布于脊椎动物的肠道和很多种类的节肢动物中。大多数沙门菌为Ⅱ相菌株,具有鞭毛相变换的特征,这与其毒力和逃避宿主免疫监视有关。综述了沙门菌鞭毛相变换的研究进展,为进一步研究沙门菌的致病机制提供了理论依据。     

福建省综合科技进步的发展态势与对策研究

利用全国科技进步统计监测数据,对福建省2001～2008年的综合科技进步水平进行评价与分析,并与全国及东部11省份进行比较分析;运用灰色模型对福建和全国科技进步的发展趋势进行预测,从而指出福建省科技进步发展中的不足,提出相应的对策建议。     

奶牛临床型乳房炎病理组织学观察

对几例临床型乳房炎乳腺组织的病理组织进行了观察,结果表明,临床型乳房炎乳腺组织的肥大细胞都主要分布在血管附近、腺泡和腺管间,说明肥大细胞的生理功能可能要通过血管反应改变微循环的状态来实现,其释放的介质进入血管发挥作用,同时募集的多形核白细胞可以游走出血管.乳房炎组织各部位的肥大细胞数量高于正常乳腺组织,说明随着病情的加剧,肥大细胞的数量会增多.     

EMMPRIN mediates matrix metalloproteinase 9 expression and monocyte migration: evidence from EMMPRIN knockdown by RNA interference

AIM: Although the evidence indicates that extracellular matrix metalloproteinase inducer (EMMPRIN) is closely associated with matrix metalloproteinase (MMP) expression in tumor cells, tumor invasion and metastasis, no direct proof that EMMPRIN regulates MMPs in monocytes, especially in the atherogenic milieu is observed. Here we tested this hypothesis by examining MMP-9 expression in macrophages/foam cells and monocyte migration through EMMPRIN knockdown by siRNA. METHODS: The methods of qPCR and Western blotting were used to detect the suppressions of EMMPRIN mRNA and protein expression in macrophages and foam cells transfected with EMMPRIN-specific siRNA. The protein expression of MMP-9 in macrophages and foam cells was also determined. Monocyte migration after EMMPRIN knockdown was observed by a Transwell assay. RESULTS: EMMPRIN knockdown by siRNA markedly abolished the MMP-9 expression by 50% and 40% in macrophages and foam cells, respectively. Migration induced by chemotactic factor MCP-1 and VEGF was significantly attenuated (P<0.05) in monocytes treated with EMMPRIN-siRNA. CONCLUSION: The protein expression and secretion of MMP-9 are down-regulated by EMMPRIN knockdown during monocyte differentiation into macrophages and foam cells. Moreover, EMMPRIN siRNA treatment also prevents monocyte migration. Thus, EMMPRIN plays a key regulatory role for MMP activity and monocyte migration, making it a potential target for pharmacological intervention of atherosclerosis.     

Expression of SARS-CoV functional receptor angiotensin-converting enzyme 2 in nephritic epithelium of primates

AIM: To investigate the expression of angiotensin-converting enzyme 2 (ACE2) in nephritic epithelium of primates. METHODS: The expression of ACE2 in Vero E6 cells was detected by the techniques of RT-PCR and immunocytochemistry (ICC) techniques. The distribution of ACE2 protein in kidney tissues of two Rhesus monkeys and two normal human cases was observed by immunohistochemistry (IHC) techniques. RESULTS: Vero E6 cells were found to express both ACE2 mRNA and protein. ACE2 protein was mainly located in epithelium of proximal tubules of Rhesus monkey and human kidney. CONCLUSION: The expression of ACE2 in epithelium of primate kidney may provide the condition for SARS-CoV entry, which may be one of the reasons for inducing renal damage in SARS patients.     

辽宁省果树生产发展的优势条件

辽宁省是我国水果生产大省之一,历史悠久、种类众多,虽然在多年的栽培历史过程中出现过曲折与沉浮,但总的来说还是在不断地发展和进步。现从辽宁省环境条件、区位特点、消费市场、地方经济、技术基础、特色产业等方面概括了辽宁省果树生产的优势条件,以期能够成为辽宁果业发展的原动力。     

河南省女子监狱规矩广场景观设计分析

广场设计要结合场地条件、所处环境及本身特点,做出符合当地文化及场地文化的个性设计,才能避免“千场一面”现象。河南省女子监狱规矩广场设计的特点是：结合场地所在地的特殊性——监狱,利用“规矩”寓意的一直一曲为基础构图,创造既具有教育意义,同时简洁适用、功能多样的空间,很好地诠释了小广场设计的真谛。     

InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

High-density markers are necessary for map-based cloning of dca genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a dce rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.     

Review and Prospect on Japonica Hybrid Rice Research in Anhui Province, China

The breeding history and commercial exploitation of japonica hybrid rice in Anhui Province, China over the last three decades were reviewed. Besides, the bottleneck problems restricting the development of japonica hybrid rice in China were summarized, and corresponding technological countermeasures were proposed.     

小麦条锈病高光谱近地与高空遥感监测比较研究

 小麦条锈病是我国小麦生产中的重要病害,对该病防治的关键是做好监测工作。本研究利用ASD手持野外光谱仪[ASD FieldSpec HandHeld FR(325~1 075 nm)]和热气球在近地与高空研究了发病小麦冠层的高光谱遥感数据特征,获得了近地和对应高空2个不同平台光谱数据。经比较分析,发现高空数据与近地数据之间存在明显而有规律的变异关系,即高空获得的光谱反射率在可见光谱区域明显大于近地获得的光谱反射率。进一步对差异最显著的绿峰580 nm和黄边610 nm处数据进行回归分析,获得了高空光谱反射率值与近地光谱反射率值之间的回归模型,为进一步研究利用高平台遥感监测小麦条锈病奠定了一定的理论基础。