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AIM: To study the role of gap junction(GJ) in the pathogenesis of epilepsy. METHODS: The expression of connexin(Cx) 32 and Cx43 was evaluated in penlylenetetrazol(PTZ)-induced epilepsy rats at different time points with immunohistochemistry and real-time RT-PCR. The uncoupler of gap junction,carbenoxolone (CBX), and anti-epilepsy drug,carbamazepine (CBZ), were introduced to investigate the role of gap junction in epilepsy. RESULTS: The expression of Cx32 in the cortex and hippocampus of rats increased 2 h after PTZ administration, and was more obvious 8 h later, so was the expression of Cx43. CBX not only notably inhibited the expression of Cx32 and Cx43, but also suppressed the seizures. CBZ had no obvious effect on Cx32/43 expression. Real-time RT-PCR examination showed that the level of Cx32 mRNA went up rapidly 2 h after seizures, and began to decrease 10 h later. The level of Cx43 mRNA in PTZ group was higher than that in control group from 2 h to 5 h. CBX inhibited both the seizures and the increase in the mRNA expression of Cx32/43, while CBZ only suppressed the seizures and did not affect the expression of Cx32/43. CONCLUSION: GJ between neurons is reinforced after epileptic activities and takes part in the pathophysiological mechanism of synchronization and epilepsy. CBZ has no effect on the expression of Cx32 and Cx43, indicating that the anti-epileptic mechanism of CBZ is independent of GJ. 相似文献
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民俗主题园公众参与性评价方法初探 总被引:1,自引:0,他引:1
以民俗主题因为代表的民俗旅游近年来才逐渐兴起,而针对民俗主题园评价体系的研究就更少.现结合民俗旅游参与性强的特点,主要从公众参与性角度来设计建立评价体系,不论是评价因子,还是权重值的确定,完全由游客打分来计算,运用模糊综合法设计评价体系建立的方案,并通过实践检验结果来确认评价系统的科学性与正确性.对民俗主题园评价体系建立的方法进行初步探索,以期探寻能客观度量民俗主题园的方法. 相似文献
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YANG Sheng-xi ZHANG Xiao-yan LUTong-de HA Xiao-qin WANG Hai-yan GENG Pai-li 《园艺学报》2008,24(5):1002-1006
AIM: To investigate the expression of natural killer cell stimulation receptor D(NKG2D) in peripheral blood and the expression of major histocompatibility complex class I chain-related protein A(MICA) on the gastric carcinoma tissue and the neighboring non-cancerous tissue in Tibetan patients with gastric cancer at Qinghai plateau. METHODS: Samples of 33 patients and 20 healthy persons were collected to detect NKG2D in peripheral blood by Flow cytometry analysis. The expressions of MICA in part of the correspondent tissues were examined by RT-PCR and immunohistochemistry.RESULTS: The expression of NKG2D in the plateau of Tibetan patients with gastric cancer group (13.47 ±5.26)% was significantly lower than that in healthy groups (32.62±10.08)%. The mRNA level of MICA in gastric cancer was significantly higher than that in neighboring non-cancerous tissue (P<0.05). The expressions of MICA proteins showed a significant difference between the neighboring non-cancerous tissue (21.21%, 7/33) and the gastric carcinoma tissue (78.79%, 26/33), between the high differentiation (60.00%, 3/5) and the moderate (72.73%, 8/11), low differentiation (88.24%, 15/17). The expressions of MICA were not correlated with tumor size, sex, age, lymph node metastasis of the patients (P>0.05). Spearman rank correlation displayed that the expressions of MICA mRNA were positively correlated with the MICA protein level (r=0.903, P<0.01).CONCLUSION: The immune escape in Tibetan patients with gastric cancer at Qinghai plateau probably relates to the down-regulation of NKG2D and the up-regulation of its ligand MICA. The activity of NK cells and the anti-cancer cellular immunity level are descending in the plateau of Tibetan patients with gastric cancer. The decrease in the receptor NKG2D is a reason for the activity of NK cell descending. 相似文献
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运用柯赫氏法则对中华蚊母上发生的一种病害进行了验证,并在室内培养基上测试了4种常用杀菌剂(75%百菌清WP、80%多菌灵WP、70%代森锰锌WP和250 g/L阿米西达)对该病原菌的抑菌效果.结果表明:该病害为中华蚊母叶部的一种新病害;初步定为中华蚊母炭疽病,病原菌为半知菌亚门刺盘孢属的胶孢炭疽菌(Colletotrichum gloeosporioids).4种杀菌剂中,以75%百菌清WP抑菌效果最好,80%多菌灵WP和250 g/L阿米西这次之,70%代森锰锌WP最差. 相似文献
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