苏云金杆菌与杀灭菊酯防治红铃虫的试验

苏云金杆菌(Bailus thuringiensis)140或018工业菌剂每亩用200～255克,加杀灭菊酯20%乳剂每亩8毫升混用防治第二、三代红铃虫,虫口减退率和铃害下降率分别为80.5～93.4%和68.8～84.6%;杀灭菊酯每亩40毫升单用效果分别为96～97.2%和84.6～95.8%,都能有效控制红铃虫的危害。棉花产量测定表明,菌剂加菊酯混用使皮棉增加21.3～30.9%,菊酯单用增加11.4%。     

Modifying the response to defoliation during vegetative growth in CERES-Maize

CERES-Maize (Vl. 0) predicted no grain yield when 100% defoliation occurred during vegetative growth. This result conflicted with field observations where 100% defoliation early in the vegetative stage did not materially reduce grain yield. CERES-Maize was modified to realistically predict yields when defoliation greater than 50% occurred during vegetative growth by taking into account stem (stem proper and sheath) photosynthesis and translocation of dry matter from the stem to the developing leaves. With these modifications, predictions from CERES-Maize were in good agreement with field measurements when defoliation occurred both early and later in the vegetative stage. This modified version of CERES-Maize can be the basis of a decision support system for defoliating pests of corn where yields can be evaluated under different management strategies and climate scenarios.     

山楂缺铁黄叶病及应用硝黄铁防治研究

根据调查初步总结出山楂缺铁黄叶病的发生发展规律、致病因素及其对山楂生产的危害。用新研制的高效价廉、储运方便的硝黄铁矫治,效果明显。黄化病株复绿率40%～87%,三级病株减少36%～100%,叶绿素含量提高64%～224%,喷肥区比对照区株产提高25%。同时增加了果实中糖、酸、果胶及钙、铁含量,提高了果实品质。     

萘乙酸对大白菜钙素吸收运转及防治干烧心病的研究

本研究从补钙入手,探索防治干烧心病的方法。用0.7％CaCl_2·2H_2O对和50ppmNAA混合液在大白菜结球期叶面喷洒5次,经8年试验,对大白菜干烧心病的相对防治效果达80％以上。 在此基础上,采用~(45)Ca示踪试验的方法对防病机理做了进一步研究。结果表明,无论从根部还是从叶部加萘乙酸对根部和叶部~(45)Ca的吸收和运转均具有促进作用,并且改变了其在大白菜植株内的分配规律。对照株单位干叶重dpm值自外向内呈下降趋势(130×10~3─60×10~3);用NAA处理株则相反,自外向内呈增加趋势(120×10~3─250×10~3),说明萘乙酸具有促进钙素向叶球中内部运转的功效。这种效果在贮藏期间仍能保持。另外,~(45)Ca向直接受到NAA处理部位的运转量有明显增高的趋势。这一结果为补钙防治干烧心病提供了理论依据。     

浅谈蛋鸡育雏所需的环境条件

培育出发育良好,体重适中而健康的蛋用育成鸡是蛋用种鸡优良繁殖性能和蛋鸡产蛋潜力发挥的条件,因此,在实际生产中,培育好雏鸡是我们技术人员和广大养鸡户必须高度重视的一个重要环节,通过对我们地区的几个大养鸡户育雏阶段的观察,认为在蛋鸡育雏阶段应把握好以下几个条件。1　温度由于雏鸡体温低,体温调节机能不完善,御寒能力差,在育雏时应特别注意温度的掌握,掌握温度一是靠温度计,另外适时调节育雏温度。通过观察,温度偏高时,雏鸡远离热源,张口喘气,不断饮水,食欲下降,活动减少,体质降低;温度偏低时,雏鸡趋近热源,互相聚拢,不断尖叫,同时…     

普通番茄与秘鲁番茄杂种后代的形态,亲和性及抗性表现

继获得普通番茄‘北京早红’与秘鲁番茄杂交种后,本试验着重于对杂种后代的形态、亲和性及抗性表现的观察,杂种的多数性状为双亲中的显性性状或双亲的中间型,但也表现了一些奇异性状,如果实的颜色和花序的形状与排列通过株间的“杂交”克服了杂种的高度自交不亲和性,从而顺利得到杂种的自交后代;杂种各世代均高抗TMV 0株系和1株系,并有部分选系抗TMV 1·2株系,由此判断秘鲁番茄亲本可能具有TM~a_2基因;用南京地区CMV厥叶株系和强株系分别接种,各世代表现部分抗病或耐病。     

Detection and characterization of leptospiral antigens using a biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay and immunoblot.

A biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of antigens of Leptospira interrogans serovars in experimentally inoculated bovine urine samples was evaluated. Immunoglobulin G (IgG) from rabbits immunized with L. interrogans serovar hardjo type hardjobovis sonicated, whole cell, and formalinized-heated antigen preparations were purified by a protein A-superose column coupled to fast protein liquid chromatography, and evaluated for species specificity in the ELISA. The ELISA using each specific IgG detected as few as 10(4) leptospires of the homologous serovar hardjo diluted in phosphate-buffered saline solution with Tween 20 (PBSS-Tween 20). On immunoblot analysis of proteinase-K-digested whole cell leptospiral preparations, each IgG revealed the presence of bands specific to serovar hardjo, suggesting the presence of serovar-specific epitopes on the lipopolysaccharide molecules. The minimum number of cells of heterologous serovars pomona, grippotyphosa, bratislava, icterohaemorrhagiae and copenhageni detected by each ELISA was greater, ranging from 10(6) to 10(7). The common antigenic determinants observed on immunoblot analysis were different for each specific IgG, except for a major cross-reacting, possibly flagellar, protein doublet at approximately 36-36.5 kDa. Leptospires were equally well detected by the ELISA in both bovine urine and PBSS-Tween 20.     

人工种植沙拐枣生长发育特性及营养动态的研究

沙拐枣属蓼科沙拐枣属多年生灌木，主要分布在我国的西北地区,本文就沙拐枣人工种植后的出苗状况，生长发育特性及不同生育期营养成分变化动态等进行了研究，为我国西部大开发生态治理工程中沙拐枣的大面积推广种植提供了指导。     

Characterization of and radioimmunoassay for canine thyroglobulin

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.     

Construction and Development of the Dagangshan Forest Ecosystem Research Station

Established by the former Ministry of Forestry in 1986, Dagangshan Forest Ecosystem Research Station is one of the 14 national key sites in the field of ecosystem research. In this paper, the basic situation of Dagangshan Forest Ecosystem Station is described, including geographic location, natural conditions, biological resources, research conditions, instruments, achievement, prospects etc.