苦水玫瑰产地土壤重金属污染评价与溯源解析

探明特色农产品产地土壤重金属污染特征及其污染来源可促进特色农作物产业的健康有序发展,对于助力乡村振兴和发展乡村特色产业具有重要意义。该研究以苦水玫瑰产地土壤为研究对象,应用内梅罗综合污染指数法、污染负荷指数法和改进物元可拓模型来量化土壤重金属As、Cd、Cr、Cu、Hg、Ni、Pb和Zn的污染水平并进行对比分析,还利用正定因子矩阵模型(positive matrix factorization, PMF)进行土壤重金属溯源解析。结果表明:1)研究区土壤除Hg和Cr的均值低于兰州市和甘肃省土壤背景值之外,其余As、Cd、Cu、Ni、Pb和Zn的均值都高于二者的背景值,但所有元素的测定值均低于《土壤环境质量农用地土壤污染风险管控标准(试行)(GB 15 618-2018)》(pH值大于7.5)的筛选值。2)内梅罗综合污染指数和污染负荷指数分别处于0.71～2.02和0.64～1.48之间,均值为1.41和1.17,土壤总体上为轻度污染。改进物元可拓模型评价结果显示研究区土壤总体上处于尚清洁状态,与内梅罗综合污染指数与污染负荷指数评价结果基本一致,但改进物元可拓模型的评价结果更具有实际指导意...     

履带式收割机全田块路径跟踪算法设计与试验

针对中小型传统收割机无人化作业需求，该研究设计了一套后装式全田块自动驾驶系统。以沃得锐龙单边制动转向型履带式收割机为平台，搭建手自兼容自动驾驶系统，并进行系统特性辨识试验，明确其测控性能限制。针对测控性能限制设计一种PD-Fuzzy-BangBang组合路径跟踪算法，并进行样机集成与试验。水泥地面直线行驶试验表明，组合算法相较单一PD算法的上线距离缩短57.3%，稳态标准差缩小81.3%。全田块模拟试验证明，组合算法在理想条件下的路径跟踪最大偏差为6.00 cm，标准差为2.42 cm，样机具备全田块自动驾驶功能。实际水田收割作业试验证明，样机在车速0.7 m/s条件下，上线过程的路径跟踪最大偏差为12.00 cm，标准差为 6.18 cm，全田块不漏割，割幅利用率大于80%，满足田间作业需求。     

自然与人文相结合的森林公园景观规划探讨——以蒙山森林公园广场规划为例

以蒙山广场规划为例,通过分析公园的自然资源和人文资源及地方文化特点,根据广场的功能和城市广场的要求,提出了森林公园广场“自然、生态、绿色、个性”的规划理念,结合和利用公园丰富的植被和水资源,形成人与自然和谐,景观与功能一致的广场特色。     

盐胁迫对柳树无性系SH31离子含量及光合作用的影响

以柳树无性系SH31为试材,研究了NaCl溶液半抑制浓度胁迫下根、茎、叶中Na⁺、K⁺、Ca²⁺ 的分布规律及叶片光合特性的变化。结果表明:在NaCl溶液半抑制浓度(0.6%)下,SH31耐盐性较强;在0.6% NaCl溶液胁迫下,根部在前16 d积累大量Na⁺并拦截部分Na⁺向地上部运输;盐胁迫降低了根、茎部对 K⁺ 的吸收,而叶部却保持较高的K⁺ 含量来维持离子平衡;Ca²⁺含量在根、茎、叶中与对照的相当。在NaCl溶液半抑制浓度盐胁迫下,SH31 的净光合速率前7 d降低之后保持稳定,而气孔导度、蒸腾速率、胞间CO₂先降后升,表明前7 d的蒸腾速率下降的主要原因在于气孔限制。SH31根部对Na⁺ 选择性吸收并维持K⁺、Ca²⁺的高含量及维持较高光合作用是其重要的耐盐机制。     

鸭甲肝病毒VP1基因克隆及序列分析

通过PCR技术,从自行分离的鸭甲肝病毒JS株基因组中扩增出病毒衣壳蛋白VP1完整基因片段,并对该片段进行序列测定及分析。结果显示JS-VP1与DHAV-A的VP1基因序列酸核苷酸同源性在62.0%~64.4%之间,与DHAV-B的VP1基因序列酸核苷酸同源性在63.6%~63.8%之间,与DHAV-C的VP1基因序列酸核苷酸同源性在92.3%~98.5%之间,分析表明JS株为鸭甲肝病毒基因C型,血清型分型为韩国新型。进化树表明JS株与SD02株的亲缘关系较进,进一步说明VP1基因的核苷酸序列是DHAV基因组中的高度可变区,可作为DHAV基因型研究的标记,分离毒株为DHAV的防治及疫苗的设计奠定了基础。     

Effects of Intragastric Lycopene on Oxidative Stress of Blood after Exercise SD Rats

The experiment was conducted to study the effects of intragastric lycopene on oxidative stress blood after exercise SD rats.Thirty two SD rats with similar weight(233.40 g±6.21 g) and good health for SPF were randomized into one control groups and three trial groups with 8 rats per group.The control group was gastric fed with edible oil,the trial Ⅰ,Ⅱ and Ⅲ groups were gastric fed with edible oil intragastric with 10,20,40 mg/(kg·BW) lycopene,respectively.The pre-experiment period lasted for 10 d,and the experiment period lasted for 42 d.Swimming train six days a week and train six weeks,then that blood were collection for determination of oxidative stress after swimming train 11 hours SD rats.The results showed that lycopene concentration of plasma was increased at first and decreased subsequently,after 4 h lycopene concentration of plasma in SD rat was peak.With the increase of intragastric lycopene doses,glutathione peroxidase,total antioxidant enzyme activity and total antioxidant capacity of plasma showed a trend of rising; malondialdehyde content of plasma showed a trend of decrease with the increase of supplemental lycopene dose after exercise SD rats.Therefore,the antioxidant capacity of blood after exercise SD rats and doses of intragastric lycopene were dose dependent,antioxidant capacity of blood after exercise SD rats showed a trend of rise with the increase of lycopene dose.     

Effect of Soybean Meal Replaced by Brewer's Grain or Fermented Brewer's Grain on Growth Performance,Immune Organ Index and Intestinal Flora of Meat Geese

This experiment was conducted to investigate the effect of soybean meal replaced by brewer's grain (BSG) or fermented brewer's grain (FBSG) on growth performance,immune organ index and intestinal flora of meat geese.A total of 250 geese at 28 days of age were randomly assigned to 5 groups with 5 replicates per group and 10 geese per replicate.The geese in control group were fed a basal diet (with soybean);The geese in groups 1 and 2 were fed the basal diet with the 10% and 20% BSG replacing the soybean,respectively;The geese in groups 3 and 4 were fed the basal diet with the 10% and 20% FBSG replacing the soybean,respectively.The whole experiment period was 42 days,including three phases:15 d (early stage),14 d (middle stage) and 14 d (later stage).The results showed as follows:①From the whole experiment period,compared with control group,the ADG of geese in group 1 was significantly increased (P<0.05),the ADG and ADFI of geese in group 4 were significantly decreased (P<0.05),there were no obvious change in growth performance of geese in groups 2 and 3 (P>0.05).② Compared with control group,the thymus index and spleen index of geese in groups 1,2 and 3 were extremely significantly increased at early stage (P<0.01);At middle and later stages,the spleen index of geese in group 2 were significantly and extremely significantly increased (P<0.05;P<0.01);There were no significant differences in bursa of fabricius index of geese among all groups (P>0.05).③ Compared with control group,the number of Escherichia coli and Salmonella in cecum in groups 1 and 2 were extremely significantly decreased at early and later stages (P<0.01),while the number of Bifidobacteria and Lactobacillus were increased in different degrees;the number of Bifidobacteria and Lactobacillus in cecum in groups 3 and 4 were extremely significantly increased at later stage (P<0.01).In conclusion,10% BSG replacing soybean in the basal diet could improve the growth performance and intestinal flora of meat geese;10% FBSG replacing soybean in the basal diet could promote the growth of beneficial bacteria of meat geese,but had no effect on growth performance;While 20% replacing soybean in the basal diet could decrease the feed intake and weight gain of meat geese,hinder its growth and development.