Suberoylanilide hydroxamic acid induces apoptosis of HepG2 cells by endoplasmic reticulum stress apoptotic pathway

AIM: To investigate the effect of suberoylanilide hydroxamic acid (SAHA) on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and to explore its possible mechanism. METHODS: HepG2 cells were treated with SAHA at different concentrations for 48 h. The proliferation of HepG2 cells was detected by real-time cellular analysis. The protein levels of acetylated histones H3K9 and H3K27, glucose-regulated protein 78 (GRP78), protein kinase R-like endoplasmic reticulum kinase (PERK) and p-PERK were determined by Western blot. The cell apoptosis was analyzed by flow cytometry. RESULTS: Compared with control group, treatment with SAHA at 0.1 μmol/L and 1 μmol/L for 48 h showed no significant inhibitory effect on the proliferation of HepG2 cells, while SAHA at 6 μmol/L and 12 μmol/L significantly inhibited the proliferation of HepG2 cells (P<0.05). The results of Western blot showed that the protein levels of acH3K9, acH3K27, GRP78 and p-PERK increased significantly after treated with SAHA at diffe-rent concentrations for 48 h, while the protein level of PERK was decreased significantly (P<0.05). The results of flow cytometry analysis showed that the apoptotic rates of the HepG2 cells increased with the increase in SAHA concentration. CONCLUSION: SAHA up-regulates the acetylation of H3K9 and H3K27 in the HepG2 cells and induces apoptosis of HepG2 cells by activating the endoplasmic reticulum stress-related apoptosis pathway.     

茎瘤芥叶栽培金针菇试验

以茎瘤芥叶、棉籽壳为主要原料栽培金针菇,筛选最佳栽培配方,拟为茎瘤芥叶的循环利用提供参考依据。结果表明:金针菇最适栽培配方的质量百分数为24%茎瘤芥叶、60%棉籽壳、10%麸皮、5%玉米粉、1%石膏;以此配方栽培金针菇,每袋产量最高为248.48g、生物学效率为88.74%,投入产出比为1∶3.30,比全棉籽壳栽培金针菇降低成本21.43%,提高毛利润16.44%。     

中国2种橡胶树炭疽病菌对咪鲜胺敏感性比较

炭疽病是橡胶树上一种重要的叶部病害,主要病原有胶孢炭疽菌和尖孢炭疽菌。为了解中国各植胶区炭疽菌对咪鲜胺的敏感性,采用生长速率法室内测定来自海南、云南、广东和广西4省(区)22株橡胶树胶孢炭疽病菌和15株尖孢炭疽菌对咪鲜胺的敏感性。结果表明:供试的37株橡胶炭疽病菌菌株对咪鲜胺的EC_(50)值为0.022 0~0.272 1 mg/L,平均为(0.061 7±0.055 4)mg/L,EC_(50)最大值是最小值的12.5倍。其中89.2%参试菌株的EC_(50)值介于0~0.100 0 mg/L。根据成组t检验分析结果表明,目前中国4个植胶区的2种橡胶炭疽病菌对咪鲜胺敏感性无明显差异。除了来自海南和云南地区的尖孢炭疽菌有显著性差异外,其他来自不同省份的胶孢炭疽菌和尖孢炭疽菌对咪鲜胺敏感性均无明显差异。研究显示中国橡胶炭疽菌对咪鲜胺仍具有较高的敏感性,但菌株间敏感性有一定的差异,存在一定的潜在抗性风险。     

3-P(4S)并联机构分析与多目标性能优化

针对3-P(4S)并联平台,首先对其进行了位置反解,提出了由BP神经网络和拟Newton法相结合的混合数值法,并以此对机构进行了位置正解,求解精度可达到10-8数量级,求解时间在20 ms内。然后通过对位置解求导可得到机构动平台和分支杆件的速度和加速度。根据运动学分析的结果,应用牛顿欧拉方法构建该机构的动力学模型,并对机构的数值算例进行了动力学仿真验证。最后综合考虑机构的动力学性能、刚度性能和速度性能,分别推导了其评价指标,并应用改进的加权求和法对该机构进行多目标的尺寸优化。通过多目标的尺寸优化,该机构的动力学性能和速度性能提升了2倍,刚度性能提升了3倍。     

不同冰温温度对山楂果实生理及品质的影响

为延长山楂果实的保鲜期,提高果实贮藏品质,筛选适宜山楂果实冰温温度参数。在以普通冷藏(0±0.5)℃,以及果实温度为-0.4、-1.0、-1.6℃的4个不同温度的贮藏条件下,对贮藏期间‘敞口’山楂果实采后生理及品质变化进行研究。结果表明,-1.0℃的冰温处理降低了山楂果实的乙烯生成速率和最大生成量,减缓了果肉硬度、可滴定酸(TA)和维生素C(Vc)含量的下降,有效抑制了果肉中丙二醛(MDA)的积累,减轻了贮藏后期多酚氧化酶(PPO)活性的上升和酚类物质含量的减少,进而延缓了山楂果实的生理衰老进程,维持了山楂果实的良好贮藏品质;而在贮藏160天后,-1.6℃的冰温处理的山楂果实乙烯释放峰值显著增高,TA和Vc降解迅速,MDA含量显著上升,PPO活性明显上升和酚类物质含量迅速减少,加速了果实的褐变速率,生理伤害症状明显,品质劣变严重。综上所述,在-1.0℃的冰温条件下,山楂果实生理代谢被有效抑制且未出现生理伤害,贮藏效果最佳。     

南京城市化对极端气温的影响

为了科学认识城市化对极端气温的影响,利用南京地区3个城区气象站和3个周边乡村站1961—2005年日最高、最低气温资料,对比分析城市站和乡村站17个极端气温指数的平均值和变化趋势,并定量估算极端气温变化趋势的城市化影响及贡献。结果表明:近45年南京地区暖事件均为增加趋势,而冷事件多为减少趋势,最高气温、最低气温的平均值和极值均为上升趋势。城市站与乡村站相比,暖事件更多,冷事件更少,最高气温、最低气温的平均值和极值更大。城市化对极端气温变化趋势的影响有两大特点:城市化加速了极端气温指数的变化速率,使得暖事件、平均值、极值的上升趋势和冷事件的下降趋势变得更大;基于最低气温的极端气温指数变化趋势比基于最高气温的极端气温指数受城市化影响更显著。城市化贡献表现为:暖事件大于冷事件,最低气温极值和平均值大于相应的最高气温极值和平均值。     

贵州省黔东南地区草地贪夜蛾寄生性天敌资源调查初报

草地贪夜蛾Spodoptera frugiperda(J. E. Smith)于2019年5月入侵贵州黔东南地区,严重威胁当地农业生态。黔东南地区丰富的生物资源是挖掘草地贪夜蛾自然天敌的重要基础。为明确黔东南地区草地贪夜蛾天敌的发生情况,2019年5月至7月,采用五点取样法对选取的3个地点开展调查。结果表明,草地贪夜蛾在黔东南地区的主要寄生性天敌有5类,分别为白僵菌属、绿僵菌属、茧蜂科、姬蜂科和黑卵蜂属昆虫,寄生率最高分别为45.50%、57.00%、3.00%、20.00%和12.50%,寄生草地贪夜蛾幼虫期4种天敌综合控制力最高达96.50%。本研究可为草地贪夜蛾天敌资源挖掘与利用提供重要参考。     

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR.     

捆草机电气控制系统的设计研究——基于MCGS与单片机技术

以呼伦贝尔市蒙拓农机科技股份有限公司生产的9YG-1.3型圆草捆打捆机为样机,针对其控制部分存在自动化程度低、草捆边缘布网太松、操作繁琐及需人工手动计数等问题,运用昆仑通态MCGS编程软件和Keil u Vision4软件,设计了一款以单片机系统为控制核心,以MCGS触摸屏为人机界面,配备各种相应传感器和控制输出硬件的自动控制系统。该系统通过人机监控界面,实现了对捆草机运行状况的实时监控。试验结果表明:本控制系统有效地解决了捆草机的灵活布网问题与计数问题,并可以实时进行停车、开仓门、关仓门与报警声亮提示,提高了捆草机的工作效率和自动化水平。