全文获取类型
收费全文 | 31209篇 |
免费 | 1778篇 |
国内免费 | 2865篇 |
专业分类
林业 | 3340篇 |
农学 | 4259篇 |
基础科学 | 2121篇 |
5265篇 | |
综合类 | 8235篇 |
农作物 | 1827篇 |
水产渔业 | 1452篇 |
畜牧兽医 | 6084篇 |
园艺 | 999篇 |
植物保护 | 2270篇 |
出版年
2024年 | 90篇 |
2023年 | 361篇 |
2022年 | 768篇 |
2021年 | 1037篇 |
2020年 | 968篇 |
2019年 | 1022篇 |
2018年 | 829篇 |
2017年 | 1145篇 |
2016年 | 984篇 |
2015年 | 1249篇 |
2014年 | 1284篇 |
2013年 | 1711篇 |
2012年 | 1835篇 |
2011年 | 1982篇 |
2010年 | 1932篇 |
2009年 | 1703篇 |
2008年 | 1696篇 |
2007年 | 1594篇 |
2006年 | 1614篇 |
2005年 | 1421篇 |
2004年 | 764篇 |
2003年 | 702篇 |
2002年 | 611篇 |
2001年 | 676篇 |
2000年 | 714篇 |
1999年 | 801篇 |
1998年 | 678篇 |
1997年 | 574篇 |
1996年 | 482篇 |
1995年 | 481篇 |
1994年 | 459篇 |
1993年 | 430篇 |
1992年 | 415篇 |
1991年 | 368篇 |
1990年 | 336篇 |
1989年 | 291篇 |
1988年 | 238篇 |
1987年 | 176篇 |
1986年 | 132篇 |
1985年 | 82篇 |
1984年 | 83篇 |
1983年 | 80篇 |
1982年 | 63篇 |
1981年 | 71篇 |
1980年 | 65篇 |
1979年 | 76篇 |
1976年 | 59篇 |
1975年 | 52篇 |
1974年 | 61篇 |
1973年 | 75篇 |
排序方式: 共有10000条查询结果,搜索用时 281 毫秒
91.
选用90只Hy-Line W-98母鸡(26周龄)进行为期8周的试验,研究来源于酵母硒的有机硒做为产蛋鸡硒来源的作用效果.试验结果表明,在蛋鸡日粮中添加酵母硒可以有效提高鸡蛋中的硒含量. 相似文献
92.
93.
鸡白血病病毒抗原ELISA试剂盒的研制和应用 总被引:5,自引:2,他引:3
以双抗体夹心酶联免疫吸附试验(DAS-ELISA)为基本原理,成功地研制出鸡白血病病毒抗原ELISA试剂盒,该试剂盒与国外同类试剂盒相比,具备相同的特异性和敏感性,对RAV-1纯化样品的最小检出量可达0.78ug/ml,且敏感性高于直接补体结合试验。经初步应用发现,我国商品种鸡群ALV阳性率为0.7-14%,在部分SPF鸡群中未检出阳性鸡。 相似文献
94.
Selective muscarinic receptor antagonists were used to identify muscarinic receptor subtypes in equine trachealis strips. The M1 receptor antagonist pirenzepine (10–7 mol/L to 3 × 10–5 mol/L) and the M3 receptor antagonist 4-diphenylacetoxy-N-methylpiperidine (4-DAMP, 10–9 mol/L to 3 × 10–7 mol/L3) dose dependently inhibited the contractile responses to electrical field stimulation (EFS) and exogenous acetylcholine (ACh). Schild plots yielded a pA2 value for pirenzepine vs ACh of 6.75 ± 0.09, which is consistent with the affinity for M2 or M3 receptors, and a pA2 value for 4-DAMP vs ACh of 8.47 ± 0.09, which is in agreement with the affinity for M3 receptors. The M2 receptor antagonist gallamine (10–5 mol/L and 10–4 mol/L) did not affect the response of trachealis to exogenous ACh and low-frequency EFS (0.1–2 Hz) but decreased the responses to high-frequency EFS (4–16 Hz). These results suggest that the muscarinic receptors mediating contractions induced by ACh in equine tracheal smooth muscle are of the M3 subtype. The lack of an increase in the response to EFS following gallamine suggests that functional prejunctional inhibitory M2 receptors are not present on the cholinergic nerves innervating equine tracheal smooth muscle. 相似文献
95.
L Mishu G Callahan Z Allebban J M Maddux T C Boone L M Souza C D Lothrop 《Journal of the American Veterinary Medical Association》1992,200(12):1957-1964
Recombinant canine granulocyte colony-stimulating factor (rcG-CSF) was administered to clinically normal dogs, cyclic-hematopoietic dogs, and dogs undergoing autologous bone marrow transplantation, to determine whether rcG-CSF could be used to stimulate WBC production and function in normal and neutropenic dogs. To the normal dogs, rcG-CSF was administered by SC injection at rates of 1 microgram/kg of body weight, q 12 h; 2 micrograms/kg, q 12 h; or 5 micrograms/kg, q 12 h. A significant dose-dependent increase in the WBC count resulted from the stimulation of bone marrow progenitor cells. The increased WBC count was characterized by mature neutrophilia and monocytosis. Neutrophil myeloperoxidase and phagocytic activity were normal in rcG-CSF-treated normal dogs, demonstrating the production of normal functional neutrophils in response to rcG-CSF treatment. Recombinant canine G-CSF prevented neutropenia and associated clinical signs but did not completely eliminate the cycling of neutrophils in cyclic-hematopoietic dogs when it was administered at rates of 1 microgram/kg, q 12 h, and 2.5 micrograms/kg, q 12 h. The time to bone marrow reconstitution was not decreased in dogs treated with rcG-CSF at a rate of 2.5 micrograms/kg, q 12 h, for 13 days following autologous bone marrow transplantation. On the basis of our findings, we suggest that treatment with rcG-CSF is an effective way to stimulate myelopoiesis in dogs, but that the dose of rcG-CSF required to stimulate WBC production will vary depending on the cause of neutropenia. Recombinant canine G-CSF should be useful in stimulating production and maintaining function of WBC for treatment of clinical diseases seen commonly in veterinary practice. 相似文献
96.
97.
98.
1989~1990年选用陆地棉(G.hirstum L.)北农1号品种和海岛棉(G.barbadenseL.)海7124品种为材料,对棉纤维发育后期的纤维素形成过程进行了研究。结果表明:①随着棉纤维的发育 Mg~(2+),K~+-ATP 酶活性逐渐下降。②Mg~(2+),K~+-ATP 酶活性的最适 pH 值为6.5。Mg~(2+),K~+ATP 酶活性随[Mg~(2+)],[K~+]和[NADH]/[NAD~+]的增大而变大。③Mg~(2+),K~+ATP 酶活性变化与还原性糖含量变化呈显著正相关(r=0.811~*),与纤维素含量变化呈极显著负相关(r=-0.900~(**))。④Mg~(2+),K~+-ATP 酶参与了纤维素的形成过程。 相似文献
99.
Renal specimens from 6 mink with encephalitozoonosis were studied by light and electron microscopy and immunohistochemistry. The glomeruli of affected kidneys had a mesangioproliferative glomerulonephritis which was characterized by an increase in mesangial cells and matrix in most glomeruli. Some glomeruli were partially or completely sclerosed. There were protein or granular casts in the cortical and medullary tubules. Interstitial nephritis, vasculitis and tubular cysts were found. Electron microscopy demonstrated extensive matrix and increased cellularity in the mesangial areas. Glomeruli showed segmentally thickened or wrinkled capillary basement membranes. Electron dense deposits were found in the glomerular basement membranes and mesangium. Peroxidase-anti-peroxidase immunohistochemistry demonstrated that IgG and IgM positive material was present as granular deposits in the glomerular basement membrane and occasionally in the mesangium. 相似文献
100.
红杂18是以红213为母本,黄苗试材9375为父本,配制成适宜罐藏加工和长途运销的番茄一代杂种。具有高抗TMV、中抗CMV、果实硬度高、抗裂、耐压、耐贮运、果实加工性状优良等特点。已在新疆、甘肃、宁夏、广西、云南等省区的加工番茄生产基地和远运外销生产基地推广649.8hm2。 相似文献