全文获取类型
收费全文 | 6357篇 |
免费 | 521篇 |
国内免费 | 32篇 |
专业分类
林业 | 330篇 |
农学 | 437篇 |
基础科学 | 54篇 |
974篇 | |
综合类 | 696篇 |
农作物 | 275篇 |
水产渔业 | 387篇 |
畜牧兽医 | 3019篇 |
园艺 | 93篇 |
植物保护 | 645篇 |
出版年
2020年 | 62篇 |
2019年 | 113篇 |
2018年 | 210篇 |
2017年 | 218篇 |
2016年 | 173篇 |
2015年 | 193篇 |
2014年 | 191篇 |
2013年 | 347篇 |
2012年 | 260篇 |
2011年 | 313篇 |
2010年 | 245篇 |
2009年 | 207篇 |
2008年 | 301篇 |
2007年 | 266篇 |
2006年 | 232篇 |
2005年 | 198篇 |
2004年 | 192篇 |
2003年 | 194篇 |
2002年 | 145篇 |
2001年 | 234篇 |
2000年 | 179篇 |
1999年 | 169篇 |
1998年 | 69篇 |
1997年 | 74篇 |
1996年 | 53篇 |
1995年 | 59篇 |
1994年 | 44篇 |
1993年 | 51篇 |
1992年 | 103篇 |
1991年 | 105篇 |
1990年 | 108篇 |
1989年 | 106篇 |
1988年 | 85篇 |
1987年 | 80篇 |
1986年 | 74篇 |
1985年 | 58篇 |
1984年 | 64篇 |
1983年 | 52篇 |
1981年 | 44篇 |
1980年 | 48篇 |
1979年 | 72篇 |
1978年 | 52篇 |
1977年 | 52篇 |
1976年 | 59篇 |
1975年 | 52篇 |
1974年 | 61篇 |
1973年 | 75篇 |
1972年 | 44篇 |
1971年 | 45篇 |
1969年 | 43篇 |
排序方式: 共有6910条查询结果,搜索用时 15 毫秒
931.
932.
Nakashiba T Young JZ McHugh TJ Buhl DL Tonegawa S 《Science (New York, N.Y.)》2008,319(5867):1260-1264
The hippocampus is an area of the brain involved in learning and memory. It contains parallel excitatory pathways referred to as the trisynaptic pathway (which carries information as follows: entorhinal cortex --> dentate gyrus --> CA3 --> CA1 --> entorhinal cortex) and the monosynaptic pathway (entorhinal cortex --> CA1 --> entorhinal cortex). We developed a generally applicable tetanus toxin-based method for transgenic mice that permits inducible and reversible inhibition of synaptic transmission and applied it to the trisynaptic pathway while preserving transmission in the monosynaptic pathway. We found that synaptic output from CA3 in the trisynaptic pathway is dispensable and the short monosynaptic pathway is sufficient for incremental spatial learning. In contrast, the full trisynaptic pathway containing CA3 is required for rapid one-trial contextual learning, for pattern completion-based memory recall, and for spatial tuning of CA1 cells. 相似文献
933.
Liu P Guo W Jiang Z Pu H Feng C Zhu X Peng Y Kuang A Little CR 《The Journal of agricultural science》2011,149(2):159-169
The effect of high temperatures (above 25°C) on starch concentration and the morphology of starch granules in the grains of wheat (Triticum aestivum L.) were studied. Wheat plants of cultivars Yangmai 9 (weak-gluten) and Yangmai 12 (medium-gluten) were treated with high temperatures for 3 days at different times after anthesis. The results showed that the starch concentration of grains given a heat-shock treatment above 30°C were lower than those developing at normal temperature in both cultivars. High temperature lowered starch concentration due to the decrease of amylopectin. Under the same temperature, the effect of heat shock from 6 to 8 days after anthesis (DAA) was the greatest, whereas from 36 to 38 DAA the effect was the least. The effects of high temperatures after anthesis on starch-pasting properties were similar to those on starch concentration, especially after 35-40°C treatments. The size, shape and structure of starch granules in wheat grains (determined by electron microscopy) after heat shock were visibly different from the control. When given heat shock during development, the starch granules in mature wheat grains were ellipsoid in shape and bound loosely with a protein sheath in Yangmai 9, while they were damaged and compressed with fissures in Yangmai 12, indicating the differences in resistance to high temperature between cultivars. Ratios of large (type-A) and small (type-B) starch granules significantly decreased under heat shock, which limited the potential sink size for dry matter deposition in the grain. 相似文献
934.
F Ronning C Kim DL Feng DS Marshall AG Loeser LL Miller JN Eckstein I Bozovic Z Shen 《Science (New York, N.Y.)》1998,282(5396):2067-2072
An angle-resolved photoemission study is reported on Ca2CuO2Cl2, a parent compound of high-Tc superconductors. Analysis of the electron occupation probability, n(k), from the spectra shows a steep drop in spectral intensity across a contour that is close to the Fermi surface predicted by the band calculation. This analysis reveals a Fermi surface remnant, even though Ca2CuO2Cl2 is a Mott insulator. The lowest energy peak exhibits a dispersion with approximately the &cjs3539;coskxa - coskya&cjs3539; form along this remnant Fermi surface. Together with the data from Dy-doped Bi2Sr2CaCu2O8+delta, these results suggest that this d-wave-like dispersion of the insulator is the underlying reason for the pseudo gap in the underdoped regime. 相似文献
935.
I. A. Abdou A. A. Abou-Zeid M. R. El-Sherbeeny Z. H. Abou-El-Gheat 《Plant foods for human nutrition (Dordrecht, Netherlands)》1972,22(1):29-35
The antimicrobial activities of the crude juices ofAllium sativum, Allium cepa, Raphanus sativus have been found to be strongly active onEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi andBacillus subtilis, while the crude juices ofCapsicum frutenscens andEruca sativa were active only onEscherichia coli, Salmonella typhi andBacillus subtilis. The crude juice ofAllium kurrat was active onEscherichia coli andBacillus subtilis. The active antibacterial substances were easily isolated by different organic solvents.
Zusammenfassung Die antimikrobiellen Aktivitäten der rohen Säfte vonAllium sativum undAllium cepa wurden als stark befunden gegenEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi undBacillus subtilis, während die rohen Säfte vonCapsicum frutescens undEruca sativa nur gegenEscherichia coli undBacillus subtilis aktiv waren. Der rohe Saft vonAllium kurrat erwies sich als aktiv gegenEscherichia coli undBacillus subtilis. Die aktiven antimikrobiellen Substanzen waren durch verschiedene organische Lösungsmittel leicht zu isolieren.
Résumé Les activités antimicrobiennes des jus crusd'Allium sativum, Allium cepa, Raphanus sativus ont été trouvées actives en affectantEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi etBacillus subtilis, mais les jus crus deCapsicum frutescens etEruca sativa affectent seulementEscherichia coli, Salmonella typhi etBacillus subtilis. Le jus crud'Allium kurrat affecteEscherichia coli etBacillus subtilis. L'active substance antibactérienne est isolée facilement par dissolvants organiques différents.相似文献
936.
Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South-East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a novel LCDV isolate was identified and characterized. Based on the sequence of LCDV major capsid protein (MCP) and DNA polymerase gene, we found that the causative agents from different species of diseased groupers were the same one and herein were uniformly defined as grouper LCDV (GLCDV). Furthermore, H&E staining revealed that the nodules on the skin were composed of giant cells that contained inclusion bodies in the cytoplasm. Numerous virus particles with >210 nm in diameter and with hexagonal profiles were observed in the cytoplasm. In addition, phylogenetic analysis based on four iridovirus core genes, MCP, DNA polymerase, myristoylated membrane protein (MMP) and ribonucleotide reductase (RNR), consistently showed that GLCDV was mostly related to LCDV-C, followed by LCDV-1. Taken together, our data firstly provided the molecular evidence that GLCDV was a novel emerging iridovirus pathogen in grouper culture. 相似文献
937.
J. W. Huang L. X. Tian Z. Y. Du H. J. Yang Y. J. Liu 《Fish physiology and biochemistry》2007,33(2):167-172
This study was conducted to evaluate the effects of dietary thiamin on the physiological status of the juvenile grouper, Epinephelus coioides. Graded levels of thiamin (0.08, 0.50, 2.12, 3.15, 4.63, 12.37 mg thiamin kg−1 diet) were fed to grouper juveniles (mean weight: 16.97 ± 0.14 g) for 10 weeks. Although fish fed the thiamin-deficient (TD)
diet showed no obvious symptoms of thiamin deficiency or increased mortality, those fed the lowest doses of thiamin (0.08
and 0.50 mg thiamin kg−1 diet) had significantly decreased transketolase activity in the liver. In addition, the level of liver thiobarbituric acid
reactive substances in fish fed the TD diet was 33–67% higher than that in fish with the thiamin-supplemented diet. There
were no significant differences in superoxide dismutase activity between the different groups of fish. 相似文献
938.
Costa JZ Adams A Bron JE Thompson KD Starkey WG Richards RH 《Journal of fish diseases》2007,30(7):419-426
The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1-32, 91-162 and 181-212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181-212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents. 相似文献
939.
ANDRIJ Z. HORODYSKY DAVID W. KERSTETTER ROBERT J. LATOUR JOHN E. GRAVES 《Fisheries Oceanography》2007,16(3):240-256
Researchers have applied numerous techniques to improve billfish stock assessments, including habitat‐based models that incorporate behavioral and oceanographic parameters to standardize historical catch‐per‐unit‐effort time‐series data. These methods have allowed researchers to account for significant changes in the depths of pelagic longline (PLL) gear deployments over time. This study presents habitat‐use data recovered from high‐resolution 5‐ and 10‐day pop‐up satellite archival tags (PSATs) attached to 47 surviving white marlin released from commercial and recreational fishing gears offshore of the U.S. East Coast, the northern Caribbean, and Venezuela between 2002 and 2004. Data recovered from transmitting tags indicated that white marlin spent nearly half of their time associated with warm, near‐surface waters (<10 m). All fish displayed frequent short duration (mean: 39.8 min) vertical excursions from surface waters to depths averaging 51 m. Qualitative and multivariate classifications of data from completely transmitted movements of surviving white marlin revealed two major types of descents: one pattern was characterized by deep ‘V’‐shaped excursions of relatively short duration (mean: 23.4 min) while the other featured descents that were more broadly ‘U’‐shaped and confined to a specific depth range for an extended period of time (mean: 75.8 min). Based on the frequency, persistence, and patterns of these vertical movements, white marlin appear to direct a considerable proportion of foraging effort well below surface waters, a behavior that may account for relatively high catch rates of white marlin on some deep‐set PLL deployments. 相似文献
940.