Calcium homeostasis and its relationship to superoxide production in blood and milk neutrophils of lactating goats

Polymorphonuclear neutrophils (PMN), which comprise over 70% of the somatic cells in goat milk, are a major cellular component of innate immunity in the goat mammary gland. However, the function of milk PMNs is modified after diapedesis compared to PMNs in blood. As many aspects of PMN activity depend directly on intracellular Ca²⁺ concentration ((Ca²⁺)_i), the present study aimed to determine the changes in Ca²⁺ homeostasis of milk PMNs from lactating goats compared to autologous blood PMNs, and to examine the significance of these variations to the immuno-competency of milk PMNs. The intracellular Ca²⁺ store of freshly prepared milk cells was estimated from the elevation of (Ca²⁺)_i after ionomycin treatment, which was found to be significantly less than blood PMNs. Replenishment of the intracellular Ca²⁺ store in milk cells after intracellular Ca²⁺ depletion by Bapta-AM followed by spiking with 2.5 mM Ca²⁺ for 20 min was also compared to that of blood PMNs, showing that after depletion/spiking the intracellular Ca²⁺ store in milk cells was much less than blood PMNs. The production of superoxide anion (O₂^?) in vitro in response to (Ca²⁺)_i-dependent or (Ca²⁺)_i-independent modulators was used to evaluate the relevance of altered Ca²⁺ homeostasis on the immuno-competency of milk cells compared to blood PMNs. The results indicated that milk cells produced similarly low levels of O₂^? as blood PMNs when treated with ionomycin. However, the amount of O₂^? produced by milk cells in response to phorbol 12-myristate 13-acetate (PMA) stimulation, although greater than ionomycin treatment, was significantly less than that of blood PMNs. The capacity for O₂^? production by both cell types in response to PMA reverted to the resting state with use of the protein kinase C (PKC) inhibitor, staurosporine. In conclusion, the current study demonstrated an irreversible shortage of intracellular Ca²⁺ in the milk PMNs of lactating goats compared to blood PMNs. It also showed that preliminary O₂^–production, primed by ionomycin treatment, remained unchanged in milk PMNs, despite the shortage in intracellular Ca²⁺, but decreased O₂^? production capacity, mediated via the PKC pathway, in milk PMN. It is suggested that the defects in Ca²⁺ homeostasis in milk PMNs of lactating goats is partially attributable for the post-diapedesis functionality modifications.     

我国丝绸面临的困境及成因浅析

丝绸业是以茧丝绸为纽带 ,以出口创汇为主的跨越农工贸的外向型行业 ,现在我国的蚕茧和生丝产量分别约占世界总产量的78%和 72 % ,丝类、绸缎和丝绸服饰的出口量分别约占世界贸易的 90 %、50 %和 45% ,1 950～ 1 998年间丝绸出口创汇达 42 2 .1 2亿美元 ,在世界丝绸业和国民经济中占有重要的地位。1　我国丝绸业面临的困境80年代后期 ,我国丝绸行业高速发展 ,产量大幅攀升 ,出口量迅猛增加 ,但至 90年代中期 ,全行业发生前所未有的亏损 ,近来 ,以加入ＷＴＯ为契机 ,行业情况有所好转 ,但也仍在困境中艰难跋涉 ,发展前景不容盲目乐观。自 1 9…     

强化防微措施 提高蚕种质量

衡量蚕种质量的主要指标有蚕品种的杂交率、良卵率、家蚕微粒子病的带毒率,其中毒率的高低对蚕种质量影响很大.目前,我国在有望加入世界经济贸易组织的利好消息刺激下,茧丝绸行业有所复苏,蚕业又将进入一个新的发展时期.在本行业处于衰兴交替之时,我们种场又将面临一次新的挑战.种场只有加强对原蚕饲养基地的技术投入和资金投入,采取有效的防微措施,提高蚕种质量,才能在竞争中获胜.     

转基因家畜生物反应器浅谈（续）

转基因家畜生物反应器浅谈（续）陈庄，魏平华（广东省农科院畜牧研究所广州５１０６４０）２转基因家畜生物反应器研究的进展目前，转基因动物研究有三大焦点：一是转基因方法研究；二是利用转基因动物来研究动物基因的表达调控机制；三是利用转基因动物生产非常规畜牧产...     

日本兽医器械监管对我国的启示

在世界兽医器械产业格局中,日本占据着非常重要的地位,市场份额超过15％,技术水平处于世界顶端.日本的兽医器械产业法律完善,权责分明,产品质量和安全性管理流程清晰.我国畜牧业和宠物医疗市场的发展对兽医器械的数量和质量都提出了更高要求.因此,调查研究日本的兽医器械监管情况,对我国的行业监管具有重要借鉴价值.本文介绍了日本兽...     

功能性饲料在动物健康养殖中的应用研究进展

功能性饲料是一种新型的绿色环保饲料,具有维护动物健康、保证畜产品安全和降低养殖污染等特殊功效.文章就近几年来国内外有关功能性饲料开发和应用等方面的研究进行概述,为进一步研究提供理论参考.     

全混合日粮营养水平对肉牛育肥效果的影响

采用完全随机单位组设计方法,设计并开展试验,评价TMR营养水平对肉牛增重效果、产肉性能和经济效益的影响,结果表明:提高TMR营养水平可以提高肉牛日增重8.79%、采食速度、降低采食量0.65%;可以改善屠宰性能,提高肉牛屠宰率0.47%、净肉率1.28%、胴体产肉率1.46%及优质切块肉的比重;可以提高饲料报酬;两种经济效益分别提高159.53元、385.79元。综合以上结果得出,提高TMR营养水平可以提高肉牛增重效果、产肉性能及经济效益。     

动物及动物产品公路运输监督现状与对策

公路是动物及动物产品运输的一条重要途径,我国在一些主要公路干线上设立了临时公路运输检疫监督站(点),对运输动物及其产品的过往车辆进行查证验物、消毒等检疫监督。但随着公路运营能力的日益增强和各地对公路运输监督检查站管理上的差异,在公路运输监督检查中存在的问题也日益复杂。     

苜蓿皂甙对蛋鸡生产性能、屠体指标、胆固醇及血清脂质的影响

试验选用288只400日龄海兰褐商品蛋鸡,随机分为4个处理,每组72只鸡,设4个重复,每个重复18只鸡,采用单因素方差设计。在玉米-豆粕型日粮基础上添加0、30、60、90mg/kg的苜蓿皂甙进行60d的饲养试验,研究苜蓿皂甙对蛋鸡生产性能、屠体指标、胆固醇及血清脂质的影响。结果表明:日粮中添加苜蓿皂甙对产蛋率、平均蛋重、耗料量和料蛋比没有显著影响(P>0.05);60mg/kg和90mg/kg苜蓿皂甙能够显著降低腹脂率(P<0.05),60mg/kg能够提高半胫膛率(P<0.05)和降低肝脏胆固醇浓度(P<0.05)。添加组血清中总胆固醇极显著降低(P<0.01),60mg/kg和90mg/kg苜蓿皂甙组甘油三酯和低密度脂蛋白质显著降低(P<0.05),高密度脂蛋白有所提高(P>0.05)。综合分析得出,日粮中添加60mg/kg的苜蓿皂甙效果最佳。     

犬眼吸吮线虫病诊治

犬眼吸吮线虫病是由丽嫩吸吮线虫寄生于第三眼睑和结膜下引起,常导致犬眼部损伤,笔者诊治4例犬眼吸吮线虫病,犬只康复,现将诊治情况报道如下。