为减少取样误差,提高小麦根系研究的精准性,于2018-2020年分别采用立方体取样法(CSM)、根钻法(CK1)、挖掘法(CK2)对西农979、周麦27进行不同生育时期不同土层根系生长发育和活性的比较研究.结果表明,相较于CK1和CK2处理,CSM处理的根干质量、根长、根表面积和根体积测定值较高,20~40cm 土层中不同处理上述指标测定值的差异大于0~20 cm 土层,且在生育后期不同取样方法上述指标测定值之间差异较大.相较于CK1和CK2处理,CSM处理的根系活性测定值较高,通过不同取样方法测定的根系活性差异较大,尤其是在生育前期,而生育后期差异缩小;全生育期不同土层中根系活性的差异较大,表现为0~20 cm 土层高于20~40 cm 土层,而20~40 cm 土层中不同取样方法下根系活性测定值的差异大于0~20 cm 土层.相关性和回归分析结果表明,不同土层中不同取样方法根系性状测定值间呈极显著正相关;通过回归方程的建立,可根据CK1、CK2处理的根系性状测定值估算CSM处理根系性状测定值,从而对CK1、CK2的测定值进行矫正.,比较而言,立方体取样法虽然操作要求高,但获取的根样代表性强,根系性状调查分析精准度高、误差小. 相似文献
Aquaculture research has focused on polysaccharides as they are among the most promising new-generation immunostimulant used to control aquatic diseases. The aim of this study was to investigate the immunomodulatory effects of fermented wheat bran polysaccharides (FWBPs) on juvenile common carp (Cyprinus carpio). Carps were fed different FWBP amounts (0%, 0.1%, 0.2%, and 0.4%) for 8 weeks and then their skin mucus and serum immune parameters, hepatopancreas antioxidant indicators, and immune-related gene expression in the intestines, kidneys, and spleen were measured. The skin mucus IgM levels significantly increased in 0.1% FWBP group, but decreased in 0.4% FWBP group. The skin mucus protease and the serum alkaline phosphatase activities increased significantly in the 0.2%, 0.1%, and 0.4% FWBP groups, respectively. The serum total Ig levels increased noticeably in the 0.1% and 0.2% FWBP groups. The highest and lowest serum lysozyme activities were observed in the 0.1% and 0.4% FWBP groups, respectively. The hepatopancreatic total superoxide dismutase activity was higher in the 0.1% FWBP group than in the control. The malondialdehyde levels decreased significantly in the 0.2% and 0.4% groups. The intestinal mRNA levels of the LZM-C and IL-10 genes were significantly higher in the 0.2% than in the 0.4% FWBP group; TNF-α was significantly upregulated in the 0.1% group. The gene expression in the kidneys did not differ significantly among the treatments, except for a significant increase in the IL-10 expression in the 0.1% treatment. Significantly elevated expression of LZM-C in 0.2% group and IL-10 in 0.1% group was observed in the spleens. TNF-α and IL-1β were significantly downregulated in the 0.4% group. These results suggest that FWBPs could be used as immunostimulant feed additives in common carp cultures.