Generation of insulin-producing cells from PDX1 and NKX6.1 gene-modified bone marrow mesenchymal stem cells

AIM: To differentiate bone marrow mesenchymal stem cells (BMSCs) into functional insulin-producing cells to produce sufficient pancreatic islet cells for transplantation. METHODS: Recombinant adenovirus vectors carrying PDX1 and NKX6.1 genes were constructed and the bone marrow mesenchymal stem cells were infected by the recombinant adenovirus combined with several cytokines for differentiation. The expressions of PDX1, NKX6.1 and insulin and C-peptide in the differentiated bone marrow mesenchymal stem cells were detected by RT-PCR and Western blotting. After the differentiated bone marrow mesenchymal stem cells were transplanted into subrenal capsule of diabetic mice, cell morphology of the grafts as well as their secretion of insulin and C-peptide were detected. Besides, regulating capacities of grafts on the blood glucose level of the diabetic mice were also detected. RESULTS: BMSCs induced by recombinant adenovirus (pAdxsi-CMV-PDX1/CMV-NKX6.1) and several cytokines showed positive dithizone staining and the expressions of β-cell related molecule such as insulin and glucose transporter 2 were detected by RT-PCR, which showed a sustaining and stable expression. The similar results were showed by Western blotting, immunohistochemical staining and indirect immunofluorescence. The insulin secretions in the cells stimulated with glucose at concentrations of 5.5 and 25 mmol/L in the experimental group were (1 240.4±109.3) mU/L and (3 539.8±245.1) mU/L, respectively, and were significantly higher than those in control group. Moreover, transplantation of the cells to STZ mice in treatment group made serum glucose recover to normal level. CONCLUSION: PDX1 and NKX6.1 gene-modified bone marrow mesenchymal stem cells differentiate into insulin-producing cells in vitro. When these cells transplanted into STZ induced diabetic mice, their serum glucose could return to the normal level and they could live well. Thus this is a promising method for diabetes treatment.     

Effects of apolipoprotein (a) on vascular smooth muscle cells proliferation and cell signal transduction pathway

AIM: To investigate the effect and the mechanism of apolipoprotein (a) ［apo (a)］ on proliferation of vascular smooth muscle cells (VSMCs). METHODS: All VSMCs used in experiments were serial subcultured from primary cells and were identified by immunohistochemistry staining of α-actin. Cell growth assay was observed as cell counting and MTT assay. Western blotting was also employed to detect the related mechanism. RESULTS: All cells used in experiments were confirmed as VSMCs. Although apo (a) enhanced VSMCs proliferation, this effect was attenuated by anti-integrin αⅤβ3, LM609. Use these reagents alone had no effect on VSMCs growth. The results of Western blotting demonstrated that focal adhesion kinase (FAK) was activated by apo (a) and the expression of total or phosphorylated transforming growth factor β1 (TGF-β1) was also decreased. However, these effects described above were all blocked by LM609. CONCLUSION: Apolipoprotein (a) enhances VSMCs proliferation and this effect is mediated by integrin αⅤβ3, which activates FAK and attenuates TGF-β1 and phospho-TGF-β1 expression.     

北京市小龙门国家森林公园大型真菌名录

北京市小龙门国家森林公园大型真菌资源丰富。在保护区内共采集标本212号,鉴定出47种,隶属于2个门、7个目、20个科、47个种。     

基于控制局域网的拖拉机液压悬挂电控系统

根据拖拉机悬挂系统机电液一体化的控制方式要求,以单片机C8051F040/1为主控制器,设计了基于CAN总线的拖拉机电控液压悬挂电子控制系统,开发了系统的CAN网络、硬件电路、软件程序,提出了控制系统的CAN传输信息帧的身份码(ID)和数据的编写方式,并实现了通过CAN网络对拖拉机悬挂的操作以及对其工作状态的监测与调控.     

柴油机功率强化前后气缸盖的温度场模拟与试

对功率强化前后的柴油机分别进行了性能仿真计算,进而确定了缸盖温度场计算的边界条件;利用有限元软件对气缸盖进行三维稳态热分析,并考虑了冷却水沸腾换热的影响;在该柴油机的单缸试验机上进行了稳态温度场测量试验,分析了试验和计算结果;得到了火力面边界条件的设置规律、稳态温度场随工况的变化关系和单缸机与多缸机的稳态温度场差别.     

基于多体接触碰撞的松软地面车轮沉陷仿真

提出了一种大规模多体系统接触碰撞理论与车辆地面力学理论相结合的仿真方法,并基于多体动力学仿真平台开发了车轮土壤相互作用仿真模块。基于该方法建立的土壤动力学模型和车轮土壤接触碰撞模型,进行了车轮沉陷的仿真。根据记录,在普通配置台式计算机上模拟土槽系统30 s的运动,仅需20 min。在相同实验条件下,仿真结果与土槽实验结果趋势一致,仿真曲线与土槽实验曲线吻合良好。由此表明该仿真算法能够满足越野行驶仿真的需要。     

温室环境下黄瓜采摘机器人信息获取

研究基于近红外图像的黄瓜果实与茎叶的信息表达方法,有效实现了近色系生物信息的图像识别。分析了黄瓜采摘深度图像信息的特点,通过建立基于灰度相关与极线几何相结合的匹配策略实现了双目视觉下的黄瓜抓取点的立体匹配和三维重建。研究温室环境下不同时间光照强度变化特点,建立了光照分析模型,提高了不同光照条件下的导航线提取的适应性。试验表明机器人视觉系统能有效识别、定位果实的空间位置,定位误差控制在±5mm以内。     

高精度气动比例压力阀设计与特性分析

设计了一种两级阀芯活塞式结构的以比例电磁铁为控制元件,采用电反馈闭环控制的高精度气动比例压力阀。该阀输出压力在0.6MPa内连续可调,且稳态精度可达0.25 kPa。建立了比例阀完整的非线性动态模型,分析了主要物理和几何参数对系统动态特性和控制性能的影响;构建了输出因子调整的模糊自适应比例加积分控制器,并利用ATmega16单片机实现了压力在设定范围内的快速高精度控制。实验结果表明,该阀在设计压力范围内具有良好的压力和流量特性;和传统PI算法相比,输出因子调整的模糊自适应算法在改善系统响应和控制稳定性的同时,显著提高了稳态控制精度,具有较高的效率和鲁棒性。     

流固耦合作用对离心泵内部流场影响的数值计算

采用双向同步求解的方法对离心泵内流场和叶轮结构响应进行联合求解,研究了叶轮流固耦合作用对离心泵内部流场的影响.流场模拟基于Reynolds时均化N-S方程和标准k-ε两方程湍流模型,采用多重坐标系法;结构响应基于弹性体结构动力学方程.并将计算所得的流道网格变形、流场静压和速度的分布以及径向力等结果与非流固耦合计算的流场进行对比分析.分析结果表明,流固耦合作用使得流体和固体区域计算网格发生微小变形,这不仅会改变流体对固体载荷的分布,而且会影响结构对流体的做功作用,从而影响流场的分布;叶片相对隔舌不同位置时,叶轮出口处和蜗壳流道内流场的静压分布变化趋势不同;流场速度变化主要出现在叶片和叶轮出口附近;各时间点上径向力的大小和方向变化较明显.     

PASSAT领驭2.0自动变速箱偶尔出现紧急运行状况检修分析

结合PASSAT领驭2.0轿车偶尔出现自动变速箱Ⅲ挡运行的紧急运行状况故障,分析了故障码、有关传感器电路,以及故障产生的原因,据此找出了故障点,并排除了故障。