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Cheng-Zhe Ren Hui-Min Gao Jun Dai Wen-Zhuo Zhu Fei-Fei Xu Yun Ye Xiao-Ling Zhang Qiao Yang 《Marine drugs》2022,20(5)
Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliella alba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate. 相似文献
3.
滴头流道中颗粒物质运移动态分析与CFD模拟 总被引:5,自引:0,他引:5
采用CFD模拟技术,对迷宫式滴头流道内不同质量浓度的悬浮颗粒运动情况进行数字化模拟计算,并采用两相流动力学理论对迷宫式滴头结构尺度下的悬浮颗粒的随流性进行了分析,研究结果表明,采用DPM模型对悬浮颗粒分布特点的模拟结果与已有试验数据吻合度较高,可以作为模拟的手段;水流中颗粒质量浓度对滴头内部流场有着较强的反作用,应在模拟过程中予以重视;根据Stokes数的计算结果,10%左右的颗粒随流性差,可以认为导致滴头堵塞的物质主要来自于这一部分颗粒。 相似文献
4.
车用永磁式缓速器设计中漏磁影响因素分析 总被引:2,自引:2,他引:0
以优化设计和减少漏磁为目标,运用磁路分析法建立永磁式缓速器的漏磁场模型,得到设计参数的解析式。由于参数之间的复杂函数关系,采用区间数学算法来优化永磁式缓速器的设计参数,将影响漏磁系数的非线性方程组化简为线性方程组。选取满足设计要求的目标参数——转子鼓磁感应强度Bm、气隙磁感应强度Bg,ave、漏磁系数K的最佳取值区间。使用区间迭代法,求出自变量参数的取值区间和权数pi。运用Matlab语言编程直接搜索合理的设计参数,减少了计算量和循环次数。三维有限元漏磁分析和试验均证明了设计参数选取的合理性。结果表明:永磁体高度HPM、气隙ge对漏磁系数K影响较大;区间数学Tschernikow算法在永磁式缓速器设计中是有效的。 相似文献
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6.
Yufei Zhu Jianfei Zhao Chenxu Wang Fei Zhang Xinhuo Huang Zhouzheng Ren Xin Yang Yanli Liu Xiaojun Yang 《畜牧与生物技术杂志(英文版)》2022,(1):280-287
Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p... 相似文献
7.
在绒山羊冷冻精液稀释液中分别添加0mmol/L、2.5mmol/L、5.0mmol/L、7.5mmol/L和10.0mmol/L五个梯度的谷氨酰胺,以确定冷冻效果最好的谷氨酰胺添加浓度,优化绒山羊冷冻稀释液配方。结果表明:添加5.0mmol/L谷氨酰胺组精子的活率、顶体完整率和生存指数均极显著或显著地高于其他试验组(P0.01,P0.05),而畸形率极显著或显著低于其他试验组(P0.01,P0.05);在脂质过氧化反应和抗氧化酶活性方面,添加5.0 mmol/L组MDA浓度极显著低于其他试验组(P0.01),而CAT活性极显著高于其他试验组(P0.01)。总之,在稀释液中添加5.0mmol/L谷氨酰胺,在冷冻—解冻过程中能起到很好的保护精子的作用。 相似文献
8.
Based on the distribution of 64 locations in wattle natural distribution in Australia and the data collected from 61 plantations in 30 more countries, 5 important factors were selected for determining the suitable climatic requirements for the growth of wattle. The experience of plantation wattle in China for over 30 years has been summarized. According to the comparison of climate and geographic distribution of wattle in China and other countries, China is a suitable country for growing wattle. Based on the results of our investigation, a division of suitable areas for growing wattle in China was made. Key points of planting method in each area were suggested. 相似文献
9.
猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察 总被引:7,自引:0,他引:7
采用Annexin V-FITC/PI双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒(PRRSV)实验感染SPF猪不同时期外周血单核细胞和肺泡巨噬细胞感染Annexin V-FITC^+/PI^-细胞群(早期凋亡细胞群)。结果显示,PRRSV感染猪外周血单核细胞和肺泡巨噬细胞Annexin V-FITC^+/PI^-细胞群的表达率均明显高于正常对照猪,感染后24h表达率达最高值。 相似文献
10.
OBJECTIVE: To evaluate biochemical analytes in vitreous humor collected after death in West Indian manatees (Trichechus manatus latirostris). DESIGN: Prospective study. ANIMALS: 73 West Indian manatees of various ages and both sexes. PROCEDURE: The condition of manatee carcasses submitted for routine postmortem examination was categorized as fresh, moderately autolyzed, or severely autolyzed. The eyes were removed, frozen for shipping, and thawed on arrival at the laboratory. Vitreous humor was extracted, and various biochemical analytes were measured. Values for each analyte were compared with published reference ranges for corresponding biochemical analytes in serum. Values were also compared among the carcass condition groups. RESULTS: Creatinine and urea nitrogen concentrations in vitreous humor were significantly higher in severely autolyzed carcasses than in fresh carcasses. Potassium concentrations in vitreous humor were significantly higher in moderately autolyzed carcasses than in fresh carcasses but were highly variable in severely autolyzed carcasses. These data were consistent with those reported in other species. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that measurement of biochemical analytes in vitreous humor of manatees is feasible and has potential for assisting in the postmortem diagnosis of certain metabolic, renal, and nutritional diseases; determining severity of autolysis at time of necropsy; and determining the time of occurrence of human-induced trauma. 相似文献