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许永恒 《内蒙古林业调查设计》2007,30(2):9-12
文章从分析湖南省西洞庭湖国际重要湿地的现状入手,按照国家湿地保护管理的总体目标、基本要求,对湖南西洞庭湖国际重要湿地保护工程规划以及湿地保护管理的相关问题进行了探析。 相似文献
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优质黄芪新品系比较试验与经济性状综合分析 总被引:1,自引:0,他引:1
为山东省优质黄芪新品种的鉴定和推广种植提供依据,以传统黄芪栽培品种为对照,研究了泰黄芪1号新品系在泰安和莱芜的生育期、植物学性状、抗病性、产量及经济性状。结果表明:泰黄芪1号生育期比传统黄芪延长9~12d;生长旺盛,整齐度高,须根、侧根少,外观品质及商品性好;平均产量达1187.6kg·666.7m-2,比对照平均增产12.02%,差异达显著水平(P<0.05);白粉病和根腐病发病率最低,分别为27.15%和7.7%。选育的新品系泰黄芪1号生育期长、产量高、抗病性强且表现高产和稳产,可进一步参加省区域试验。 相似文献
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以生物工程技术表达及120 g/L SDS-PAGE电泳纯化Nonapeptide突变体,取制备的Non-apeptide突变体进行抗新城疫病毒(NDV)的鸡胚试验、鸡体内抗NDV试验。结果表明,当Nonapeptide突变体基因产物浓度达4μg/mL~6μg/mL,对鸡胚保护率均达到100%,感染鸡胚全部存活;Nonapeptide突变体基因产物浓度大于4μg/mL,对NDV有很好的抑制作用,鸡用药后3 d体内检测不到NDV,低剂量组(2μg/mL)也有较好的抑制NDV作用,鸡用药后5 d体内检测不到NDV。Nonapeptide突变体基因产物具有NDV多克隆抗体相似活性,能够抑制鸡胚中和组织培养中NDV的繁殖,具有中和、抑制NDV吸附作用。 相似文献
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重离子辐照阿维菌素产生菌的诱变选育 总被引:2,自引:1,他引:1
应用不同剂量12C+6重离子束对出发菌株ZJAV-A1进行辐照选育,并应用高效液相色谱法测定发酵液中有效组分B1a的含量.结果表明:当重离子束12C+6离子的辐照剂量为50Gy时,B1a含量的延变率最高,为34.2%;诱变后的菌落共培养出4种菌型,即草帽型、馒头型、光秃型和皱缩型,其中,草帽型高产菌株ZJAV-Y203的发酵液中B1a组分比出发菌株提高了51.43%.说明利用12C+6重离子束辐照处理阿维菌素产生菌,可以得到高产B1a组分的诱变菌株,其诱变效果显著. 相似文献
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YUAN Wei-wei LIN Qiu-xiong ZHU Jie-ning LI Xiao-hong FU Yong-heng LIU Xiao-ying TAN Hong-hong DENG Chun-yu SHAN Zhi-xin 《园艺学报》2014,30(7):1172-1178
AIM:To investigate the effect of caspase-8 small hairpin RNA (shRNA) on attenuating apoptosis of human mesenchymal stem cells (hMSCs). METHODS:Two recombinant plasmids for over-expression of caspase-8 shRNA, pAd-Cap8 shRNA1 and pAd-Cap8 shRNA2, were constructed. Caspase-8 mRNA was determined in pAd-Cap8 shRNA-transfected human HEK293 cells by Q-PCR. The screened pAd-Cap8 shRNA was used to construct the recombinant adenovirus plasmid, which was linearized and transfected into HEK293 cells for packaging and amplification of the recombinant adenovirus rAd-Cap8 shRNA. The expression of caspase-8 at mRNA and protein levels was determined by Q-PCR and Western blotting. Annexin V/PI staining and determination of caspase-8 activity were performed to assess apoptosis of hMSCs under the conditions of serum deprivation and hypoxia. The mRNA expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), insulin-like growth factor 1 (IGF-1), Bcl-2 and Bcl-xL was analyzed by Q-PCR. RESULTS:The pAd-Cap8 shRNA, which efficiently inhibited caspase-8 expression, was screened by Q-PCR. The recombinant adenovirus plasmid for caspase-8 shRNA was constructed and used to package and amplify the recombinant adenovirus (rAd)-Cap8 shRNA successfully. rAd-Cap8 shRNA-mediated caspase-8 shRNA markedly inhibited caspase-8 expression in hMSCs. Over-expression of caspase-8 shRNA by infection of rAd-Cap8 shRNA also efficiently decreased the apoptotic rate and caspase-8 activity in hMSCs under the conditions of serum deprivation and hypoxia, with up-regulation of the mRNA expression of HGF, IGF-1 and Bcl-2. CONCLUSION:Caspase-8 shRNA attenuates hMSC apoptosis under the conditions of serum deprivation and hypoxia. 相似文献
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