红芪愈伤组织中黄酮代谢途径的探讨

在红氏供试材料中苯丙氨酸转氨酶活性在分化愈伤组织中最高，但游离苯氨丙酸含量在分化愈伤组织中并无明显降低，说明游离氨基酸的积累主要来自蛋白质的分解。黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的ＳＤＳ－ＰＡＧＥ平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。     

筹建期的高职学校图书馆建设——以温州科技职业学院图书馆为例

以筹建温州科技职业学院图书馆为例,阐述了图书馆从中职到高职的机构设置、人才队伍、制度建设、服务层次、系统升级、馆舍布局、资源建设等方面的转变,为兄弟馆迎接"摘筹"评估提供实践借鉴.     

大豆抗营养因子对生长蛋鸡生产性能、氮代谢和小肠内胰蛋白酶活性的影响

为了观察不同梯度大豆抗营养因子（Ａｎｔｉｎｕｔｒｉｔｉｏｎａｌｆａｃｔｏｒｓ、简称ＡＮＦｓ）对不同生理阶段的生长蛋鸡的影响，本试验采用４８０只海兰褐蛋用雏鸡，以熟豆饼日粮为对照，从３或９周开始给饲３个水平的生大豆日粮（４％，９％和１３％），每期每个日粮４个重复。在３～８周和９～１８周观察雏鸡的采食量，日增重和饲料效率。在第８和１７周分别测定各组的粗蛋白质和干物质的代谢率。并于８、１３和１８周末每个重复随机抽取试鸡两只屠宰，观察小肠内胰蛋白酶活性的变化。结果表明，３个水平的大豆ＡＮＦｓ对育雏期雏鸡的增重没有影响，育成期对照组的增重明显高于采食生大豆组（Ｐ＜０．０５），大豆ＡＮＦｓ使饲料效率降低，但对氮和干物质的代谢影响不显著；８、１３和１８周龄雏鸡小肠内胰蛋白酶活性亦无显著变化。     

ST14/MT-SP1 influences expression of MT2-MMP and TIMP-2 to promote invasiveness of colorectal cancer cells

AIM: To evaluate how ST14/MT-SP1 overexpression alters invasiveness of colorectal cancer cells.METHODS: Human full length ST14/MT-SP1 gene was transiently transfected into colorectal cancer (RKO) cell lines.The expression products were purified by chromatography on Ni2+-affinity resin column and analyzed for gelatinase activity by gelatin zymography.Cell invasion and migration were determined by ECM invasion assay in vitro.RNA was isolated from stable ST14-transfected RKO cells and a cDNA microarray was utilized to detect the change in expression of MMPs and TIMPs.Real-time quantitative PCR was used to validate the change of expression.RESULTS: The full length ST14/MT-SP1 was transfected and expressed in RKO cells.The expressed protein showed a gelatinase activity.In addition,invasiveness of RKO was significantly enhanced by ST14/MT-SP1 overexpression (P<0.01).Blockage of ST14/MT-SP1 resulted in a decrease in the invasiveness of SW480 and SW620 (P<0.01).Furthermore,MT2-MMP (MMP-15) expression was significantly up-regulated (2.5-fold change) and TIMP2 down-regulated (0.35-fold change) by ST14/MT-SP1 overexpression in RKO.CONCLUSION: ST14/MT-SP1 overexpression results in an increase in invasiveness of colorectal cancer RKO cells.Increased invasiveness is due to an increase in the gelatinase activity of ST14/MT-SP1 and a change in up-regulated MT2-MMP and down-regulated TIMP-2 expression.Therefore,ST14/MT-SP1 overexpression enhances invasiveness of colorectal cancer cells.     

Plasma enteroglucagon and neurotensin levels in gnotobiotic calves infected with enteropathogenic and non-enteropathogenic viruses

Five gnotobiotic calves were each infected with five viruses. Each calf was inoculated with coronavirus at seven days old, followed by astrovirus, Newbury agent, parainfluenzavirus type 3 and rotavirus at intervals of two weeks. Three of the viruses were enteropathogenic (bovine coronavirus, bovine calici-like virus and bovine rotavirus) and two were not (bovine astrovirus and parainfluenzavirus type 3). Plasma levels of the peptide hormones enteroglucagon and neurotensin and faecal output were measured daily and xylose absorption was studied before and after each infection. A close correlation was found between a rise in plasma enteroglucagon and neurotensin and infection with enteropathogenic viruses. The three enteropathogenic viruses caused increased daily faecal output, and elevated plasma levels of enteroglucagon and neurotensin, while the non-enteropathogens did not. The calici-like virus and rotavirus but not the coronavirus caused xylose malabsorption.     

小麦中微量元素测定方法的研究

本实验对小麦中多种微量元素测定所采用的样品处理方法，溶液酸度，以及共存元素的影响进行了研究，为选择最佳分析条件提供了依据。     

金华市婺城区木材加工产业存在的问题与对策

通过对金华市婺城区木材加工产业发展现状的调查分析，婺城区木材加工产业主要存在散、乱、低、危等问题，提出促进婺城区木材加工产业健康发展的对策建议，重点采取科学规划、规范管理、培育龙头、政策扶持等措施做强婺城区木材加工产业。     

油用向日葵新品种F53的选育

F53是辽宁省农业科学院作物研究所利用外引油葵雄性不育系HS55A与自选不育恢复系5069组配而成的油葵单交种。属中熟油用型杂交种,具有高产、高油、生育期适中、抗性好、适应性广等特点。2005年经国家向日葵品种鉴定委员会鉴定通过。     

理工科大学生学习心理压力及影响研究

通过对503名在校大学生心理压力的问卷调查，比较了文理科学生不同的学习心理压力，重点分析了理工科学生的学习心理压力、影响、应对方式。笔者认为，由于学科特点，理工科学生在大学生活中面临的学习压力较大。必须把握理工科学生学习心理压力的特点及规律，适时有效地进行引导和教育。