Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis

Variability of high molecular weight glutenin subunits (HMW-GS) was studied in198 accessions of Ae. Tauschii (2n=2x=14, DD) by sodium dodecyl sulphate(SDS-PAGE) and acid polyacrylamide gel electrophoresis (A-PAGE) and capillary electrophoresis (CE). A high allelic variation of HMW-GS, including some novel x- and y-type subunits and variable subunit combinations were observed. One accession(TD159) showed a x-type null form. The results by A-PAGE analysis revealed that the subunits Dx5 ^t and Dy10 ^t encoded by Glu-D ^t 1 locus in Ae. tauschii were different in relative mobilities in comparison with the subunits Dx5 and Dy10 found in bread wheats, whereas they had the same mobilities, respectively, when separated by SDS-PAGE. The higher resolution of Ae. tauschii HMW-GS separated by CE method showed two clear peaks in accordance with x- and y-type subunits, respectively,except the accession TD151 which possessed only subunit Dy12.1^*t. The electro elution time of the x-type and y-type subunits were about 13–14 and 7–8minutes, respectively. Characterization of wheat HMW-GS was facilitated by using CE which provides high resolution and increases the speed of analysis in conjunction with the traditional gel electrophoretic methods. A total of 42HMW-GS alleles were identified, among which were several alleles not presently detected in bread wheats. Hence Ae. tauschii is potentially a valuable genetic resource for quality improvement of bread wheat. This revised version was published online in August 2006 with corrections to the Cover Date.     

神舟3号飞船搭载的树莓试管苗超微结构及其RAPD分析

已有研究表明,植物进入太空后,其生长、发育、细胞组织结构以及基因组均发生不同程度的变化.例如,大豆[1]及三叶草[2]受空间微重力的影响使其生长明显加快,许多植物的形态及细胞学行为也受太空环境影响[3～10];另外,空间微重力可以改变植物基因表达水平[11～14],半胱氨酸等放射性保护剂的应用进一步证实太空射线可损伤植物染色体[15].但目前有关太空习行对植物试管苗亚细胞超微结构及其基因组影响的报道较少.本文对神舟3号飞船搭载并成功回收的树莓试管苗进行了细胞亚显微结构及RAPD分子标记的初步分析.     

锦绣黄桃在安顺的引种表现及主要栽培技术

锦绣黄桃是上海市农业科学院选育的鲜食与加工兼用晚熟黄桃新品种,其果个大、外形美观、营养丰富。2016年安顺市农业科学院引种到安顺种植,经过连续几年观察,其表现出适应性强、丰产性好、品质优良。初步认为可在安顺地区推广种植。     

磁化NaCl胁迫溶液对水稻种子萌发的影响

研究磁化强度对NaCl胁迫下水稻种子萌发和幼苗生理的影响。方法：用不同强度的磁化NaCl胁迫溶液浸种并喷洒水稻种子及幼苗,测定种子的发芽指标和幼苗的生理指标。实验结果表明：经0.05T的磁化强度处理后,与对照相比,水稻种子萌发的发芽率、发芽势、发芽指数和幼苗中可溶性糖含量有明显提高,达到显著性差异水平;溶液经不同磁化处理后,过氧化物酶活性增强,表现为酶带增多,酶带加粗;对酯酶同工酶影响不太明显。结论：研究表明一定强度的磁化处理可以提高盐胁迫下水稻种子的萌发能力和幼苗的抗盐能力。     

哈尔滨农民回迁居住区户外景观设计模式初探

回迁居住区是城市化推进的产物之一。回迁居民在这里的生活模式和物质环境都与其本来状态存在许多矛盾与冲突,使得回迁者常出现“回而不适”现象,对农民生活、心理产生极大负面影响。文章以哈尔滨市城郊失地失宅农民为研究对象,通过对哈尔滨市现有回迁居住区进行实地调研,总结出农民回迁者审美的特异性和行为活动需求及农民回迁居住区户外空间环境存在的主要问题,最后提出满足于哈尔滨失地失宅农民审美情趣及行为需求的农民回迁居住区户外空间设计策略,让农民回迁者有一定的家园感与归属感。     

Developing Method Based on Object-oriented Modeling Techniques for Management Information System

A developing method is given which based on object-oriented modeling techniques for management information systems.The modeling techniques,especially,from CDM to PDM conversion,make it possible that the method can be used for developing such management information systems which based on relational database.The result shows that it can raise the efficency of the system developing,improve the system tability,enhance the system maintenability.     

Fault Section Diagnosis Algorithm of Distribution Networks Based On Topology Identification

An algorithm of fault section diagnosis based on topology identification for distribution networks is presented. By decomposing the topologic matrix which describing the distribution network into two parts,one part only contain the complex coupling factor ,and the other ignore the complex coupling factor. Using this method, the fault zones in distribution network can be identified and isolated efficiently, and the vertexes of the zones can also be identified automatically. This approach adapts to the changefully network structures.     

Analysis of Radial Forces on the Seal Block in a Compound Gear Pump with 3 Idle Gears

By analyzing the pressure distribution between gears and sealing blocks in compound gear pump with three idlers,the resultant hydraulic forces acting on seal blocks are obtained.The conclusion is the unbalanced resultant hydraulic forces are relatively small in magnitude.In the pump,the inner sealing edges are long and so it s Sealing effect is good,but the outer sealing effect is relatively poor because of its short sealing edges.The little unbalanced force pushes the sealing block to te pumping chamber,therefore it is advantageous to meliorating the pump's sealing effect,thereby to improving it's volumetric efficiency as well.     

人工养殖的哲罗鱼、白点鲑和美洲红点鲑血液指标

为掌握哲罗鱼、白点鲑和美洲红点鲑血液学原始数据,测定了哲罗鱼、白点鲑和美洲红点鲑血液生理、生化指标,以及在5.75 /S,9.6 /S、11.5 /S、23.0 /S、46.0 /S、115.0 /S、230.0 /S 7个切变率（γ）全血粘度和230 /S切变率血浆粘度;将3种鱼在不同切变率下的全血黏度拟合成逆函数回归曲线,其全血质量分数都呈典型幂函数变化,其曲线回归方程分别为：哲罗鱼y=12.328 x-0.135 3,R2=0.9917;白点鲑y=7.894 2x-0.104 6,R2=0.9979;y=7.505 9x-0.106 2,R2=0.9931。统计分析表明：3种鱼Fb值呈显著性差异P﹤0.05;Tp差异不显著（P>0.05）,但存在大小关系：白点鲑>美洲红点鲑>哲罗鱼;红细胞聚集性指数（A1、A2）相互之间差异不显著（P>0.05）,白点鲑与美洲红点鲑之间红细胞刚性系数差异不显著（P>0.05）,均与哲罗鱼呈显著性差异（P<0.05）。