Study on the Tissue Culture of Coleus blumei

This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine （6-BA）, α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA）. The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA （2.0 mg· L^-1） and NAA （1.0 mg· L^-1）. Shoot tips were induced on the medium containing 6-BA （4.0 mg·L^-1） and NAA （0.5 mg·L^-1）. The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA （0.1 mg·L^-1）.     

禽粪好氧堆肥发酵高温阶段微生物的分离及其作用

文章对禽粪好氧堆肥发酵高温阶段微生物数量趋势及其对堆料中主要有害物质的降解作用进行了研究。微生物数量趋势排序为细菌>放线菌>霉菌。各类微生物降解情况为:淀粉类主要由细菌降解,放线菌次之,霉菌无效;蛋白类也主要是细菌降解,放线菌和霉菌均有一定作用;填充料秸秆中的纤维素类降解主要是放线菌和霉菌,细菌并未表现出效果。     

犬新孢子虫NcSRS2基因原核表达质粒的构建

根据已发表的犬新孢子虫NcSRS2基因序列,设计1对含有EcoRⅠ和NotⅠ酶切位点的引物。以提取犬新孢子虫虫体基因组DNA为模板,应用PCR扩增获得NcSRS2 ORF基因片段,将此基因片段克隆到pMD18-T Simple载体上,用EcoRⅠ和NotⅠ双酶切该片段,回收得到含有两个酶切位点黏端的Nc-SRS2 ORF基因,将此基因片段克隆至相同酶切回收后的PGEM-4T-2原核表达载体中,获得重组质粒pGEX-NcSRS2,经PCR鉴定,限制性内切酶分析和克隆片段序列测定比较,证实了重组质粒的正确性。     

减氮条件下生物炭对烤烟根系发育及土壤微生物群落的影响

为探索减氮条件下生物炭对植烟土壤的烤烟根系生长及土壤微生物种群数量的影响,采用大田试验方式,研究减氮40％条件下添加不同用量的生物炭对旺长期烤烟根系形态指标、根系活力以及土壤微生物数量的影响.结果表明,减氮条件下施用生物炭料可有效增加烟株根系各形态指标,同时增强根系活力,并且对增加土壤酶菌和细菌数量有显著促进作用.相对...     

绿色食品企业内检员队伍建设现状及对策研究

绿色食品企业内检员是绿色食品企业产品质量的第一把关人,是企业生产管理的监督员;建设一支业务精、能力强、素质高的内检员队伍,对于促进绿色食品事业持续健康发展具有重要意义。文章在对全国绿色食品企业内检员队伍建设情况进行问卷调查和实地调研的基础上,分析评价了绿色食品企业内检员队伍建设成效,研究探讨了绿色食品企业内检员队伍建设存在的主要问题,并从把好内检员职能定位、完善内检员注册管理、加强内检员培训教育、健全内检员奖惩机制、理顺内检员管理服务体制等5个方面提出了加强绿色食品企业内检员队伍建设建议与对策。     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

异戊烯基转移酶基因导入番茄及转基因植株再生

通过根癌农杆菌（Ａgrobacteriens）介导,利用特异启动子Ｐsag12与异戊烯基转移酶（Ｉsopenyl transferase,ipt）基因构建的嵌合基因Ｐsag12－ipt对５个番茄品种进行遗传转化,结果共获得来自３１个不同外植体的４４株转化再生植株。感染子叶的分化再生及转化植株田间生长正常。对其中部分转化再生植株进行ＰＣＲ检测证明为转基因植株,并且在田间表现出生长旺盛及抗叶片衰老的特性。     

利用转基因技术育成耐贮藏番茄——华番1号

利用转基因技术将乙烯形成酶（ＥＦＥ）反义基因导入到番茄中获得耐贮藏番茄系统Ｄ２,再结合杂种优势育种方法用Ａ５３与其杂交配组育成杂种一代华番１号。该品种在常温条件（１３～３０℃）下可贮藏４５ｄ（天）左右,产量与当地主栽品种早丰相当,且品质好,适应性广。     

循环率对一维间歇入渗土壤水、氮运移分布影响的室内试验

循环率r的大小直接影响间歇入渗土壤致密层的发育程度,进而影响其减小土壤入渗率和田面糙率的作用。该文在室内模拟了地下水位为70.00 cm的非饱和土壤、灌施条件下的垂直一维间歇入渗过程,重点研究了循环率对肥液(KNO₃溶液)间歇入渗的减渗性和土壤中入渗水、硝态氮的运移分布特性。结果表明,减小循环率可使肥液间歇入渗表土致密层发育更加成熟,减渗作用明显增强,同时入渗水分、硝态氮分布于更浅层土壤中,减弱土壤硝态氮的淋失趋势。     

培养基质对丛枝菌根(AM)真菌生长发育的影响

温室条件下,以盆栽培养方法研究不同培养基质对丛枝菌根(Arbuscular Mycorrhizae,AM)真菌Glomus mosseae生长发育的影响。结果表明：不同理化性质的基质对菌根共生体生长发育的影响不同,综合考虑菌根长度、根外菌丝量及孢子数3项指标,以沙土混合物(体积比3∶1)对G. mosseae菌剂的生长发育最为有利。宿主植物菌根长度及根中的可溶性糖浓度与根外孢子数有正相关关系,而宿主植物中磷浓度与菌根真菌的生长发育也有类似的关系。说明培养基质的养分状况、水分状况、通气状况等诸多因素都会影响菌根共生体的建立和发展。宿主植物的菌根长度、根中可溶性糖浓度以及宿主植物磷浓度对菌根真菌的生长发育有显著影响。因此,工厂化AM菌剂生产中,应以沙土混合物(体积比3∶1)为生产G. mosseae菌剂的培养基质。