YWS-1型烟田挖坑施肥机的研究设计与应用

为了更好地解决“烟草三农”问题，针对我国烟草种植落后的现状和发展趋势，研究和应用具有稳定挖坑和精量施肥等功能YWS-1型烟田挖坑施肥机。通过性能和生产试验测定和效益分析证明，该机具各项技术、性能指标符合设计要求，解决了烟草种植的问题，具有一定的创新特点和较好的推广和应用价值。     

胸部高分辨率CT在新生儿胸部疾病诊断中的意义

目的 探讨胸部高分辨率CT(HRCT)在新生儿胸部疾病诊断中的意义.方法 收集69例行胸部HRCT检查的新生儿临床资料,分析新生儿胸部疾病的常见HRCT征象,并与DR平片进行对比.结果 HRCT对先天性胸肺发育异常疾病的诊断符合率明显高于DR平片(77.8％ vs 38.9％,P＜0.05);HRCT在发现新生儿重症肺炎特殊征象上明显优于胸部DR平片(71.4％ vs 4.8％,P＜0.01).结论 胸部HRCT对于肺部发育不良、纵膈病变、新生儿重症肺炎等具有较大的诊断意义.     

不同林龄枫香子代性状变异与家系选择

对布设在安徽祁门县的首批84个枫香子代测定林，分析比较了9年生与3年生两个不同林龄胸径、树高性状的遗传力和遗传增益等参数，并开展了优良家系选择。结果表明不同时期入选的家系名称、数量及名次出现较大差异，大部分家系胸径、树高性状的家系遗传力、单株遗传力及遗传增益等参数估值，表现为随林龄增大而降低。研究结果为枫香良种选育的适宜林龄提供了参考依据。     

一株乳源松鼠葡萄球菌ExhC基因的克隆及序列分析

松鼠葡萄球菌(Staphylococcus sciuri,S.sciuri)是引起奶牛乳房炎(Cow Mastitis)的病原菌之一,其主要致病因子是该菌产生的脱皮毒素C(Exfoliative Toxin C,ExhC)。为了深入了解该基因的分子特征,本试验对从乳房炎患牛牛乳中分离的一株松鼠葡萄球菌ExhC基因(Genbank登录号:MT845354)进行了克隆及序列分析。按GenBank收录的ExhC基因设计并合成引物,利用PCR对ExhC基因进行扩增,并对扩增后的ExhC基因进行测序及分析。结果表明,松鼠葡萄球菌ExhC基因的开放阅读框序列长度为837bp,共编码278个氨基酸。通过Blast模块对ExhC基因进行同源性分析,得到5株相似序列,与ExhC基因序列相比同源性均为99.04%。系统进化树图指示该基因与其余五株相似序列属于不同的分支,遗传距离较远。利用TMHMM对松鼠葡萄球菌ExhC基因837bp序列跨膜区进行预测,结果显示ExhC蛋白的第1~277位氨基酸在细胞膜外,5~25位氨基酸所在位置为跨膜区。通过ExhC氨基酸序列分析和ExhC蛋白二级结构、三级结构预测,推断第97位氨基酸插入Asn以及其他位点氨基酸的突变可能会导致松鼠葡萄球菌功能区变化和毒株毒力的相对变化,这将给松鼠葡萄球菌致病机理的研究奠定基础。     

“1237”P氏生物安全立体非洲猪瘟防控体系

在目前非洲猪瘟疫苗毒株、变异毒株、野毒株并存背景下,只做好外部生物安全已不足以达到防控非洲猪瘟的目的。因疫苗毒株隐蔽性强、检测难度大,不知道通过引种是否会将疫苗毒株带到猪场。因此,未来猪场生物安全不能只关注场外生物安全,更重要的是要运用“以猪为本”的“1237”P氏生物安全立体非洲猪瘟防控体系,即在做好“控五流”等外部生物安全的同时,更要关注饮水安全、注重猪的自身健康度、提高猪的非特异性免疫力,才能实现“与非共存,与蓝共舞”。所谓“立体防控”是指全方位的防控,而不是仅仅针对猪场大门的传统生物安全防控。     

保温型刚性护面渠道设计

在刚性面层下设置一道性能良好的保温层来防止基土冻结,可以从根本上解决冻胀破坏的问题。这种带保温层的刚性护面渠道称为保温型刚性护面渠道,根据热工原理,提出了保温型刚性护面渠道横断面设计在构造方面的要求,进一步推导出计算保温层厚度的有关公式,并给出算例,还指出了需要进一步研究和探讨的问题。     

Isolation and Identification of BPIV3 Genotype C

To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.     

Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.