青贮玉米育种和栽培的研究与探讨

主要对青贮玉米育种、栽培进行了浅析，并指出其发展前景，旨在推动我国青贮玉米育种研究再上一个新台阶。     

Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.     

基于ArcIMS的江川县森林资源信息发布系统

介绍了ArcIMS的体系结构、特点及技术优势。构建了基于ArcIMS的江川县森林资源信息发布系统,提出了系统设计的思路、功能、特点,并实现了部分功能。     

黑加仑嫩枝扦插三个时期的水分管理

文章介绍了黑加仑嫩枝扦插苗生根过程中三个时期的水分管理,可供生产实践参考。     

霜冻害对苗圃育苗质量的影响分析

分析了苗圃育苗霜冻害产生的原因,提出预防霜冻害的措施。     

冷态鼓泡流化床中木屑流化速度的研究

冷态条件下,在鼓泡流化床中采用木屑为原料,分布板在 2%～4% 不同开孔率的条件下,分别考察了原料粒径和床层高度对木屑流化速度的影响.得出结论:分布板压降和流速成二次函数关系;以木屑为原料,分布板开孔率和床层高度对临界液化速度的影响不大,粒径分布对临界流化速度随粒径的增大而增加.     

几种酚酸对毛竹种子发芽的影响

针对连续多年覆盖后雷竹林产生的衰退减产问题,从竹林覆盖物料对竹子的化感作用角度对衰退的原因进行了分析.利用外施不同浓度和种类的酚酸以及稻草、竹叶水浸液对毛竹种子进行发芽实验,以探求酚酸等化感物质对竹子生长的影响.试验结果表明,随处理液浓度的上升,几种酚酸和稻草、竹叶水浸液对毛竹种子的抑制作用增强.稻草、竹叶2种覆盖物1倍浸提母液处理后长出的种子胚根迅速失活死亡,5、10倍2种浸提母液对发芽势、胚根长度、胚根质量有促进作用.100 mg·L-1酚酸或混合酚酸液使胚根长度与对照相比减少24.49%～48.99%,胚根质量减少9.17%～38.08%;500 mg·L-1酚酸或混合酚酸液可使胚根长度减少73.48%～88.63%,胚根质量减少76.00%～90.83%.据此认为,覆盖物产生并在土壤中累积的大量酚酸可抑制竹林根系生长,并导致雷竹林经连续多年覆盖后衰退和减产.     

生活垃圾填埋场渗滤液水质变化研究

对生活垃圾渗滤液的水质特性进行了研究,并研究了填埋时间等环境条件对渗滤液水质变化的影响,表明了渗滤液成分的复杂性.