Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.     

芽孢杆菌微生态制剂对京红1号蛋鸡产蛋性能和蛋品质的影响

试验旨在研究芽孢杆菌微生态制剂对京红1号蛋鸡产蛋性能和蛋品质的影响。选择健康、体况良好的404日龄京红1号蛋鸡960只,随机分为4组,每组6个重复,每个重复40只。对照组饲喂基础日粮,试验Ⅰ组饲喂基础日粮+益倍佳(凝结芽孢杆菌),试验Ⅱ组饲喂基础日粮+赐尔健(纳豆芽孢杆菌),试验Ⅲ组饲喂添加地衣芽孢杆菌+枯草芽孢杆菌(1∶1)混合制剂的日粮,试验组微生态制剂的添加量均为300 mg/kg。试验期25 d,其中预饲期5 d。结果显示,与对照组相比,试验组蛋鸡产蛋率均显著提高(P<0.05),破、软蛋率均显著降低(P<0.05),试验Ⅰ、Ⅱ组料蛋比显著降低(P<0.05)。与对照组相比,Ⅱ组鸡蛋平均蛋重显著提高了2.25%(P<0.05);试验组Ⅰ、Ⅱ的蛋黄相对重、蛋壳相对重、哈氏单位均无显著影响(P>0.05);试验Ⅲ组蛋壳颜色为1~3级的鸡蛋比例明显增多,差异显著(P<0.05)。综上所述,添加微生态制剂赐尔健(纳豆芽孢杆菌)可显著提高京红1号产蛋率和平均蛋重,显著降低料蛋比和破、软蛋率;添加枯草芽孢杆菌+地衣芽孢杆菌(1∶1)混合制剂可使蛋壳颜色加深的鸡蛋比例显著增多。     

激光在土地保护、农作物生长及害虫防治领域应用研究进展

由于激光具有功率高、相干性好、光谱线宽窄、光谱范围广等特点,其在土地保护、农作物生长及害虫防治等领域应用广泛.文章综合国内外激光技术在农业领域的应用情况,介绍了已经获得应用的激光诱导击穿光谱仪、激光测距仪、激光雷达系统、激光补光灯等设备在土壤检测、土地平整、激光诱变育种、植物补光和虫害防治等领域发挥的作用,对激光技术对农业领域的推动作用,特别是激光技术在吉林省农业应用方面的情况进行了展望.     

地鳖虫纤溶酶的三维结构模拟与序列分析

为了研究地鳖虫(Eupolyphage sinensis Walker)纤溶酶的溶栓机理,根据地鳖虫纤溶酶成熟肽编码序列,利用生物信息学方法,对地鳖虫纤溶酶进行了结构分析,用Biosun软件的同源模建技术,模拟了其三维结构。利用GOLDKEY软件,对地鳖虫纤溶酶的氨基酸序列进行了分析,讨论了其等电点、亲水性、柔性与催化活性之间的关系。结果表明,地鳖虫纤溶酶的活性中心是组氨酸、丝氨酸和天冬氨酸3个氨基酸残基,位于球蛋白中心凹穴处,底物结合部位是丝氨酸、天冬氨酸和甘氨酸,该类酶水解纤维蛋白的机理是催化精氨酸-赖氨酸之间的肽键水解。     

千岛湖国家重要湿地非生物资源及其利用调查

2009年10月～2011年10月,对千岛湖国家重要湿地非生物资源及其利用状况进行了调查.千岛湖湿地非生物资源有水资源、旅游资源、湿地岛屿和土地资源等,湿地蓄水总量178.4×108 m3,整体水质良好,全湖富营养化程度较稳定；旅游资源有8主类23亚类181种基本类型238个旅游资源单体,其中优良级旅游资源单体29个；湿地岛屿总面积5 954.53 hm2,其中林地面积5 913.80 hm2,非林地面积40.73 hm2;库尾和库湾季节性可耕地面积随水位变化在192.1 ～1 013.8 hm2间变化,千岛湖湿地非生物资源利用主要是水资源和旅游资源,湿地旅游已发展成为淳安县的经济支柱产业.     

蒋卫杰博士：聚焦生产一线（八）——乐亭早春棚室薄皮甜瓜实用生产技术

乐亭早春棚室薄皮甜瓜种植历史悠久，种植面积达7 333.3 hm²（11 万亩），当地农民通过系列实用生产技术，采收两茬瓜，获得高产高效，其中日光温室冬春茬薄皮甜瓜每667 m² 产值可达5.5 万元。     

参麦与黄芪注射液联用治疗卡氏肺孢子虫肺炎

为研究参麦与黄芪注射液联用对大鼠卡氏肺孢子虫肺炎(PCP)的治疗效果,以地塞米松连续肌肉注射Wistar大鼠6周,建立PCP大鼠模型,用参麦与黄芪注射液皮下注射治疗患病大鼠,同时设复方磺胺甲恶唑(SMZ-TMP)治疗对照组和PCP模型阳性对照组。通过各组大鼠基本情况变化、肺组织印片检查包囊、肺组织病理切片染色观察组织变化,探讨其治疗效果。结果发现,参麦与黄芪注射液联用治疗后,大鼠饮食、饮水增加,活跃程度恢复,体重不同程度增加,肺印片Pc包囊数量比治疗前明显减少,肺组织炎症反应减轻,高剂量组治疗效果接近复方磺胺甲恶唑组。研究结果表明,参麦与黄芪注射液对肺孢子虫有明显的治疗作用。     

浅析乌兰察布地区金叶榆嫁接以及嫁后管理技术

金叶榆叶片金黄色,枝条密生,树冠丰满,生长旺盛,适合做多种造型,在园林绿化上应用较多;再者,抗寒、抗旱能力强,很适合在乌兰察布地区推广应用,为了满足绿化的需要,对该地区嫁接技术以及嫁后管理技术进行了总结,为当地金叶榆嫁接技术以及嫁后管理技术提供理论参考。