文蛤肝细胞超微结构研究

应用透射电镜技术研究了文蛤（Meretrix meretrix)肝脏的细胞组成，结果表明，文蛤肝脏主要由4种细胞构成；分泌细胞（B细胞）、储存细胞（R细胞）、吸收细胞（F细胞）和胚细胞（E细胞），描述了4种细胞的超微结构，根据观察结果和各种细胞的结构特点，指出文蛤肝脏细胞的功能，并探讨了肝脏细胞的分化序列。     

Effects of Kv1.5 on proliferation and apoptosis of rat PASMCs under hypoxia+hypercapnia condition and relationship with MAPK pathway

AIM：To investigate the effects of voltage-dependent K＋ channel 1.5 (Kv1.5) on the proliferation and apoptosis of rat pulmonary artery smooth muscle cells (PASMCs) under hypoxia+hypercapnia condition and the relationship with mitogen-activated protein kinase(MAPK) signal pathway. METHODS：The PASMCs isolated from the male SD rat were cultured under hypoxia+hypercapnia condition, and randomly divided into normal group (N group), hypoxia+hypercapnia group (HH group), hypoxia+hypercapnia+DMSO incubation group (HD group), hypoxia+hypercapnia+U0126 (an extracellular signal-regulated kinase 1/2 inhibitor) incubation group (HU group), hypoxia+hypercapnia+SB203580 (a p38 mitogen-activated protein kinase inhibitor) incubation group (HS group), and hypoxia+hypercapnia+anisomycin (an agonist of MAPK) incubation group (HA group). Cell Counting Kit-8 was used to detect the cell viability. The protein expression of Kv1.5, PCNA and Bax was detected by Western blotting. RESULTS：Compared with N group, the cell viability and PCNA protein expression in HH group and HD group were significantly raised (P<001), but Kv1.5 and Bax proteins were significantly decreased (P<0.01). No difference between HH group and HD group was observed (P>005). Compared with HD group, the cell viability and PCNA protein expression in HU group, HS group and HA group were decreased (P<0.05 or P<0.01), but Kv1.5 protein and Bax protein were raised (P<0.01), with the most significant changes in HA group. ^{CONCLUSION：}The regulation of Kv1.5 to the proliferation and apoptosis of PASMCs under hypoxia+hypercapnia condition might have a relationship with the activation of MAPK signal pathway.     

高铁客站站前广场景观设计初探——以沪杭高铁海宁西站设计为例

以海宁西站站前广场为研究对象,从项目背景、功能布局和交通流线方面进行了论述,探讨景观设计如何处理好与城市交通联结、展示地域文化、塑造门户形象的具体策略,以期能为相关项目提供可借鉴的经验。     

Role of miR-219 and TGFBR2 in pathogenesis of renal fibrosis

AIM:To study the role of microRNA-219 (miR-219) in regulation of transforming growth factor-β receptor type 2 (TGFBR2) in renal fibrosis. METHODS:The renal fibrosis patients (n=70) were selected in this stu-dy, and 20 cases of healthy people were selected as control group. RT-qPCR was used to detect the expression of miR-219 in the serum of the patients with renal fibrosis and control group, and the expression of miR-219 in NRK49F cells after stimulation with angiotensin Ⅱ(AngⅡ) was detected. The protein expression of α-smooth muscle actin (α-SMA) in the NRK49F cells transfected with miR-219 mimics after stimulation with AngⅡ was determined by Western blot. The potential target gene TGFBR2 of miR-219 was screened and verified by the method of luciferase reporter gene. RT-qPCR and Western blot were used to detected the effect of miR-219 mimics on the expression of TGFBR2 at mRNA and protein levels, and the mRNA expression of α-SMA, connective tissue growth factor (CTGF), type I collagen α1 (COL1A1) and COL3A1 in the NRK49F cells was also detected, respectively. The unilateral ureteral occlusion (UUO) mouse model was established and the expression of miR-219 in the renal tissue was monitored. The morphological change of renal fibrosis was observed in the UUO mice after injection of miR-219, and the mRNA expression levels of COL1A1 and COL3A1 were detected. RESULTS:The expression level of miR-219 in the patients with renal fibrosis was significantly lower than that in control group, and the expression of miR-219 in the UUO mice was decreased significantly (P<0.01). The expression level of miR-219 was significantly decreased in the NRK49F cells after AngⅡ stimulation, and miR-219 mimics inhibited the protein expression of α-SMA(P<0.01). miR-219 mimics had a targeted regulatory effect on TGFBR2 gene, which inhibited the mRNA and protein expression of TGFBR2. miR-219 mimics inhibited the mRNA expression of α-SMA, CTGF, COL1A1 and COL3A1. miR-219 also down-regulated the mRNA expression of COL1A1 and COL3A1 in the UUO mice and inhibited the process of renal fibrosis. CONCLUSION:miR-219 inhibits the development of renal fibrosis by inhibiting the expression of TGFBR2, which may become a new target for the diagnosis and treatment of renal fibrosis.     

平托花生草粉替代不同比例精料喂兔效果研究

为研究平托花生草粉替代不同比例精料饲喂新西兰大白兔生长效果,用平托花生草粉分别替代10%、15%、20%精料与对照（CK）共4个处理组。经45 d喂养试验,结果表明在日增重、屠体品质方面与全精料饲喂的对照组差异不显著,其中平托花生草粉替代10%精料的处理Ⅰ肉兔生长速度最快,日增重达22.56 g/d,料肉比为2.51∶1;在兔肉氨基酸含量方面,利用平托花生草粉分别替代10%、20%和30%的精料处理的兔肉氨基酸总量分别为68.91%、70.97%、68.51%,与全部精料饲喂的对照相比,替代20%精料的处理在氨基酸总量和人体必需氨基酸含量方面都得到了不同程度的提高;在经济效益方面,替代10%的精料,提高经济效益7.49%。说明可以利用平托花生草粉替代部分精料,达到同样的饲养效果,并节约饲粮,其中以平托花生草粉替代10%的处理饲养效果最好。     

印度转基因生物安全监管的法律思考

转基因技术的飞速发展使印度成为了世界上第二大棉花的生产国和出口国,并产生了巨大的经济、社会和生态效益。印度对转基因生物的安全监管日趋谨慎和科学,架构了基础性法规、技术指南、保障立法为法律监管框架,形成了三层管理体系密切配合的监管体制。完善的审批系统、专门的咨询部门、高效的监督机构都是印度转基因生物安全监管部门的职能体现,对我国相关部门的职能建设有所裨益。     

生长期能量采食水平对生长蛋鸡的影响

试验用４３２只伊莎褐商品蛋鸡,研究了三个不同能量采食水平日粮对生长、性成熟的影响。试验得出在７－９,１０－１２,１３－１５,１６－１８周内,高能量采食水平组鸡比中、低能量采食水平组鸡的日增重高２．５２％、５．４３％（Ｐ〈０．０５）,,３．３３％、１６．５９％（Ｐ〈０．０１）,８．１％、１２．９％（Ｐ〈０．０１）,２３．６％、４５．１％（Ｐ〈０．０１）,各期日增重差异显著;饲料转化效率在７－９周高能     

适合宁波地区秋季雪里红甜瓜嫁接的砧木筛选

为了筛选出适合宁波地区秋季雪里红甜瓜嫁接栽培的砧木,选取了9份砧木进行了雪里红甜瓜嫁接栽培试验,综合考察各砧木材料的嫁接成活率、嫁接苗的植株长势和嫁接对接穗果实品质及产量的影响,初步筛选出适宜宁波地区秋季种植的砧木品种。结果表明:甬砧2号嫁接成活率高,亲和力好,对接穗果实品质无影响,是宁波地区秋季雪里红甜瓜保护地嫁接栽培的适宜砧木。