河北地区猪圆环病毒二型ORF2基因片段的克隆与原核表达

以PCV2 PK-15细胞毒提取的DNA为模板,用PCR扩增PCV20RF2基因的后部约600 bp片段;对PCR产物回收纯化后与载体pMD19-T进行连接、转化,经酶切和序列分析后亚克隆到原核表达载体pET-32a中,构建重组表达质粒pET-32a-ORF2,转入大肠埃希氏菌B121(DE3)中,以IPTG诱导表达,进行SDS-PAGE电泳和Western-Blotting分析.结果表明.重组表达质粒在大肠中所表达的融合蛋白相对分子量为40 kDa,Western-blotting分析表明该蛋白具有PCV2抗原性.     

CT Real-time Analysis of Damage Evolution of Coal under Uniaxial Compression

In order to clarify the mechanism of rockburst, using the specified loading equipment and the CT machine, the real-time computerized tomography testing of coal under the uniaxial compression was completed. Through CT scanning, the clear CT images which included the stage from the microcrack clousre to the microcrack growth, bifurcation, development, failure and the post-failure stage were obtained. According to the CT values, the meso-damage evolution of coal under uniaxial compression was analysed, which provided the basis for the meso-damage evolution.     

Cellular Defence Reaction of American Cockroach , Periplaneta americana to Metarhizium anisopliae Isolate CQMa102

Injection of conidia solution of Metarhizium anisopliae isolate CQMa102 into the hemocoel of Periplaneta americana caused significant changes of the total hemocytes of the cockroach. P. americana eliminated large amount of the spores efficiently by phagocytosis and nodulation.The experiment shows that the immune system of the cockroach was hardly injured after injected with high dose spores, and the higher dose of spores was injected,the higher mortality of the insect was. However, low dose injection strengthened the immune ability of the insect in certain degree. The saline slightly induced the immune reaction of the insect.     

An Analysis of Principal-Agent of Game under the Asymmetric Information in Venture Capital

The relationship of investors and venture capitalists is an important part in venture capital. Based on asymmetric information, this paper analyzes the principal agent relationship between investors and venture capitalists with game theory. And this paper mainly discusses the choosing, controlling and incentive problem. It suggests that only the capable and self confident venture capitalists could accept the terms of the limited partnership. It also indicates that recompense mechanism which holds the interest of investors and that of venture capitalists tightly is the center of the limited partnership. Rate of yield is the key of recompense mechanism, it not only reflects the incentive on venture capitalists, also reflects the share of risk between investors and venture capitalists. At last, the market credit play an important role in venture capital.     

Nonlinear Predictive Control of Small Sag cable with Axial Displacement

An approach for the control of a small sag cable is presented.The approach is based on a nonlinear predictive control scheme that determines the required axial displacement input so that the predicted response match the desired Results.It is accomplished by minimizing the norm squared local errors between the predicted and desired values.Finally simulation results that use the new control strategy to demonstrate the effective are show.     

豇豆新品种吉豇1号的选育及栽培要点

吉豇1号是吉林省白城市农业科学院食用豆研究所于1994年从当地农家品种"黑豇豆"中选育出的豇豆新品种。该品种于2007—2008年参加吉林省芸豆区域试验,2年平均比对照品种增产,2008年参加吉林省芸豆生产试验,比对照品种增产。2009年通过吉林省农作物品种审定委员会审定。该品种粒大、整齐,具有直立抗倒伏、耐瘠薄、抗病毒病、霜霉病等优点,适合在吉林省西部及内蒙古兴安盟、黑龙江省嫩江等地区种植。     

基于农作制分区的1985—2015年中国小麦生产时空变化

Comparing spatio-temporal variation characteristics of China’s wheat production, yield, sown area and yield-area-contribution in different farming zones in the past 30 years could help improving wheat planting layout and adjusting planting structure. Concentration index, rate of change, moving of gravity and resolution of yield-area-contribution were used to analyze spatio-temporal changes of China’s wheat production and yield-area-contribution based on county wheat production statistics including sown area, production and yield from 1985 to 2015. The wheat sown area decreased obviously in Northeast farming region, Northwest farming region and South farming region and increased rapidly in Huang-Huai-Hai farming region and Yangtze Plain farming region. In Huang-Huai-Hai farming region, the concentration indexes of Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region reached to 20.64%, 25.77%, and 21.65% respectively in 2015. Wheat production increased significantly by more than 48 milliontons in Huang-Huai-Hai farming region, and nearly 8 million tons Yangtze Plain farming region but decreased by more than 2.6 million tons in Northeast farming region. In Huang-Huai-Hai farming region, wheat production was concentrated in Haihe Plain farming region, Huang-huai Plain farming region and Yuxi Hill farming region. The average wheat yield continuously improved during the study period, Huang-Huai-Hai farming region and Northwest farming region had the yield increase up to 103.5 kg hm ^-2 and 92.9 kg hm ^-2 each year. Wheat yield in Yuxi Hill farming region, Fenwei Basin farming region and Haihe Plain farming region was relatively high in Huang-Huai-Hai farming region. The yield-reduced area was mainly caused by the decreasing of sown area, while the yield-area-contribution rate was different in yield-increased area. Yield-dominant counties were reduced, area-dominant counties were increased and yield-area-dominant counties were relatively steady. In production increased area, yield-dominant and yield-area-dominant counties were the main types in Huang-Huai-Hai Farming region, area-dominant and yield-area-dominant counties were the main types in Yangtze Plain farming region. Chinese wheat production was increasingly concentrated in Huang-Huai-Hai farming region which has high and rapid increase of wheat yield over the past three decades. Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region were the most concentrated areas in Huang-Huai-Hai farming region for wheat production during this period. Wheat yield and sown area jointly promoted the increase of wheat production in Huang-Huai-Hai farming region, wheat sown area was the crucial factor to increase wheat production in Yangtze Plain farming region, especially in the north of Jiangsu, Anhui province and greater part of Xinjiang.     

云南小粒咖啡果皮粉发酵酸奶的研制

以鲜牛奶为主要原料,添加云南小粒咖啡果皮粉、蔗糖、琼脂,直投式发酵菌种为发酵剂,以酸奶感官品质评分为考察指标,在单因素试验的基础上,应用Box-Behnken试验设计优化确定最佳的云南小粒咖啡果皮粉发酵酸奶的工艺与配方。结果表明:咖啡果皮粉添加量为鲜奶量的0.63%,蔗糖为鲜奶量的7.50%,琼脂为鲜奶量的0.55%,发酵时间6.6h时,获得的发酵酸奶的感官品质评分为91分,产品组织状态均匀,口感细腻,略带有咖啡香味,感官评价最佳。     

南极磷虾中氟的研究进展

南极磷虾以其巨大的生物量和潜在的渔业资源价值越来越受到世界各国的重视。南极磷虾富含蛋白质及多种营养元素,是人类重要的生物蛋白库,但高氟含量一直是制约其加工及安全利用的瓶颈。介绍了南极磷虾中氟的存在概况,赋存形态及检测技术的研究现状,并概述了南极磷虾中氟的安全性研究进展。     

盐碱胁迫对枸杞生长及根际适应能力的影响

通过水培试验研究不同盐碱胁迫条件对枸杞生长以及根际pH值和电导率的影响。结果表明:枸杞在含有NaCl的营养液中培养,随着NaCl浓度的升高,枸杞的株高、鲜质量和干质量减少,根系对根际的pH值和电导率调节能力减弱;此外,枸杞能够在1.4%NaCl水培条件下生长,1.6%NaCl以上水培条件会导致枸杞落叶直至死亡。